FMDV.3D.107 |
FMDV.3D.107.F |
ACTGGGTTTTACAAACCTGTGA |
12.5 µL QuantiNova RT-qPCR (Qiagen, Germany), Primers 0.4 µM, Probe 0.2 µM, 3 µL amostra, water q.s. 25 µL (1) 95°C 5 minutos, (45) 95°C 10 segundos, 60 °C 60 segundos |
(7) |
|
FMDV.3D.107.R |
GCGAGTCCTGCCACGGA |
|
FMDV.3D.107.S |
FAM-TCCTT TGCAC GC-CGT GGGAC-BHQ1 |
FMDV.3D.99 |
FMDV.3D.99.F1 |
ACTGGGTTTTACAAACCTGTGATG |
12.5 µL QuantiNo-va RT-qPCR (Qiagen, Germany), Primers 0.4 µM, Sonda 0.2 µM, 3 µL amostra, água q.s. 25 µL (1) 95°C 5 minutos, (45) 95°C 10 segundos, 60 °C 60 segundos |
(24) |
|
FMDV.3D.99.F2 |
CTGGGTTTTATAAACCTGTGATGGC |
|
FMDV.3D.99.R1 |
CCACGGAGATCAACTTCTCCT |
|
FMDV.3D.99.R2 |
TGCCACAGAGATCAACTTCTCC |
|
FMDV.3D.99.R3 |
CCACGGAAATCAACTTCTCCTG |
|
FMDV.3D.99.S |
FAM-TCTCCTTTGCAC-GCCGTGG-BHQ1 |
FMDV.3D.130 |
FMDV.3D.130.F |
GGACCATACAGGAGAAGTTGA |
12.5 µL QuantiNo-va RT-qPCR (Qiagen, Germany), Primers 0.4 µM, Sonda 0.2 µM, 3 µL amostra, água q.s. 25 µL (1) 95°C 5 minutos, (45) 95°C 10 segundos, 60 °C 60 segundos |
(25) |
|
FMDV.3D.130.R |
CGCAGGTAAAGTGATCTGTAGC |
|
FMDV.3D.130.S |
FAM-CTCCGTGGCAG-GACTCGCAGT-BHQ1 |
FMDV.3D.183 |
FMDV.3D.183.F |
GAC AAA GGT TTT GTT CTT GGT CA |
12.5 µL QuantiNo-va RT-qPCR (Qiagen, Germany), Primers 0.6 µM, Sonda 0.3 µM, 3 µL amostra, água q.s. 25 µL (1) 95°C 5 minutos, (45) 95°C 10 segundos, 60 °C 60 segundos |
(26) |
|
FMDV.3D.183.R |
TGCGAGTCCTGCCACGGA |
|
FMDV.3D.183.S |
FAM-ATC CTC TCC TTT GCA CGC CGT GGG ACC AT-BHQ1 |
FMDV.IRES.145 |
FMDV.IRES.145.F |
TAA CAW GGA CCC RCS GGG CC |
12.5 µL QuantiNo-va RT-qPCR (Qiagen, Germany), Primers 0.4 µM, Sonda 0.2 µM, 3 µL amostra, água q.s. 25 µL (1) 95°C 5 minutos, (45) 95°C 10 segundos, 60 °C 60 segundos |
(27) |
|
FMDV.IRES.145.R |
TGA AGG GCA TCC TTA GCC TG |
|
FMDV.IRES.145.S |
FAM - CAT GTG TGC AAY CCC AGC ACR G - BHQ |
FMDV.3D.88 |
FMDV.3D.88.F |
ACTGGGTTTTAWAAACCTGTGATG |
12.5 µL QuantiNo-va RT-qPCR (Qiagen, Germany), Primers 0.4 µM, Sonda 0.2 µM, 3 µL amostra, água q.s. 25 µL (1) 95°C 5 minutos, (45) 95°C 10 segundos, 60 °C 60 segundos |
(17) |
|
FMDV.3D.88.R |
TCAACTTCTCCTGKATGGTCCCA |
|
FMDV.3D.88.S |
FAM-ATCCTCTCCTTTG-CACGC-Iowa Black |