Plant growth regulators on the micropropagtion of <i>Actinidia</i> cultivars

Krakhmaleva, Irina Leonidovna; Molkanova, Olga Ivanovna; Orlova, Natalia Dmitrievna; Koroleva, Olga Vasilyevna; Mitrofanova, Irina Vjacheslavovna

doi:10.1590/1413-7054202347008923

ABSTRACT

Actinidia Lindl., commonly known as kiwifruit, is a valuable berry crop. The area of commercial kiwifruit plantations is increasing; the global production of kiwifruit is about 0.62% of the total production of major fruit crops. The use of biotechnological methods, which can significantly accelerate the propagation of quality planting materials, is considered to be relevant for the propagation of this crop. In this study, we optimized the culture medium composition at the micropropagation stage for the effective cultivation of promising cultivars of A. arguta, A. kolomikta, and A. polygama. We investigated the features of Actinidia morphogenesis depending on the genotype, the concentration of 6-Benzylaminopurine (0.5, 0.8, and 1.0 mg L^-1), and plant growth regulators (6-Benzylaminopurine, meta-topolin, and 2-isopentenyladenine at a concentration of 0.5 mg L^-1) in the media Quoirin and Lepoivre. Actinidia arguta (multiplication rate of 8.0) and A. polygama (6.8) developed faster at the micropropagation stage compared to A. kolomikta (4.9). The studied Actinidia representatives were cultured most effectively on Quoirin and Lepoivre media supplemented with 0.5 mg L^-1 meta-topolin, compared to the media containing 0.5 mg L^-1 6-Benzylaminopurine and 0.5 mg L^-1 2-isopentenyladenine. The use of meta-topolin in the medium contributed to the increase in various morphometric traits, such as the height of microshoots (up to 28% depending on the species), their number (up to 52%), and their multiplication rate (up to 42%). We also recorded a high morphogenic capacity of the investigated species.

Index terms:
A. arguta; A. kolomikta; A. polygama; cytokinin; regeneration.

[1] ^*Corresponding author: seglory@bk.ru

Species	Cultivar	Sex	Fruit color	Fruit mass (g)	Fruit taste
A. arguta	Male form	male	-	-	-
	‘Solnechnyj’	male	-	-	-
	‘Zolotaya Kosa’	female	yellowish green, green	8.0	sweet, with apple flavor
	‘Rebristaya’	female	green	7.6	sweet and sour, with pineapple flavor
	‘Taezhnyy Dar’	female	green	7.6	sweet and sour, with strong pineapple flavor
A. kolomikta	Male form	male	-	-	-
	‘Komandir’	male	-	-	-
	‘Zemlyanichnaya’	female	yellowish green	2.8	sweet, with strawberry flavor
	‘Izobilnaya’	female	yellowish green, with light longitudinal stripes	3.6	sweet and sour, with strong pineapple flavor
	‘Moma’	female	yellowish green	2.7	sweet, with pineapple flavor
	‘Pamyati Kolbasinoy’	female	olive green, with light longitudinal stripes	4.3	light sweet and sour, with pineapple flavor
A. polygama	Male form	male	-	-	-
	‘Beta’	female	orange, to dark orange	3.7	like bell pepper, with pepper aroma
	‘Osennyaya’	female	dark orange	4.5	like bell pepper, with pepper aroma
	‘Perchik’	female	orange	2.7	like bell pepper, with pepper aroma

Species	6-BAP (mg L^-1)	Length of shoot (cm)	Number of shoots per explant (unit)	Multiplication rate
A. arguta	0.5 (control)	1.7 a*	1.9 b	8.0 a
	0.8	1.6 a	2.0 ab	8.0 a
	1.0	1.6 a	2.2 a	8.2 a
A. kolomikta	0.5 (control)	1.6 a	1.4 b	4.9 b
	0.8	1.6 a	1.5 a	5.1 b
	1.0	1.6 a	1.6 a	5.4 a
A. polygama	0.5 (control)	2.5 a	1.7 b	6.8 b
	0.8	2.6 a	1.9 a	7.1 a
	1.0	2.5 a	1.8 a	7.2 a

Species	Cultivar	Plant growth regulator	Length of shoots (cm)	Number of shoots per explant (unite)
A. arguta	Male form	6-BAP (control)	1.7±0.4 b*	1.8±0.6 b
		mT	2.2±0.5 a	2.1±0.7 a
		2-ip	2.4±0.4 a	1.1±0.3 c
	‘Solnechnyj’	6-BAP (control)	1.4±0.4 c	1.7±0.5 b
		mT	2.3±0.6 a	2.3±0.8 a
		2-ip	1.7±0.3 b	1.2±0.4 с
	‘Zolotaya Kosa’	6-BAP (control)	1.5±0.4 b	2.1±0.5 b
		mT	2.4±0.6 a	2.7±0.6 a
		2-ip	2.4±0.6 a	1.1±0.3 c
	‘Rebristaya’	6-BAP (control)	1.7±0.4 b	1.5±0.5 b
		mT	1.8±0.5 b	2.2±0.6 a
		2-ip	2.5±0.6 a	1.1±0.3 c
	‘Taezhnyy Dar’	6-BAP (control)	2.0±0.5 c	1.8±0.4 b
		mT	3.0±0.7 a	2.2±0.4 a
		2-ip	2.7±0.7 b	1.2±0.4 c
A. kolomikta	Male form	6-BAP (control)	1.6±0.7 a	1.6±0.5 b
		mT	1.4±0.4 a	2.3±0.5 a
		2-ip	1.4±0.5 a	1.2±0.4 b
	‘Komandir’	6-BAP (control)	1.1±0.4 b	1.9±0.6 b
		mT	1.4±0.3 a	2.3±0.6 a
		2-ip	1.5±0.4 a	1.1±0.3 c
	‘Zemlyanichnaya’	6-BAP (control)	1.5±0.5 b	1.6±0.6 b
		mT	1.8±0.2 a	2.0±0.0 a
		2-ip	1.8±0.5 a	1.2±0.4 c
	‘Izobilnaya’	6-BAP (control)	2.0±0.5 b	1.0±0.2 b
		mT	2.6±0.7 a	2.0±0.5 a
		2-ip	1.6±0.5 c	1.0±0.0 b
	‘Moma’	6-BAP (control)	1.8±0.5 c	1.1±0.3 b
		mT	3.0±0.6 a	2.3±0.8 a
		2-ip	2.4±1.0 b	1.0±0.0 b
‘Pamyati Kolbasinoy’	6-BAP (control)	2.1±0.8 a	1.4±0.5 b
	mT	1.7±0.4 b	1.9±0.6 a
	2-ip	2.4±0.6 a	1.1±0.3 b
A. polygama	Male form	6-BAP (control)	1.9±0.5 a	1.9±0.6 ab
		mT	2.0±0.5 a	2.1±0.5 a
		2-ip	2.0±0.6 a	1.8±0.6 b
	‘Beta’	6-BAP (control)	2.6±0.7 a	1.4±0.5 b
		mT	3.0±0.6 a	2.0±0.7 a
		2-ip	3.3±1.0 a	1.3±0.1 b
	‘Osennyaya’	6-BAP (control)	3.1±0.8 b	1.5±0.5 a
		mT	3.5±1.1 a	1.5±0.5 a
		2-ip	3.8±0.9 a	1.2±0.4 b
	‘Perchik’	6-BAP (control)	1.9±0.6 a	2.1±0.4 a
		mT	1.8±0.5 a	1.9±0.4 b
		2-ip	1.9±0.7 a	1.6±0.6 c

Brasil

Brasil

Plant growth regulators on the micropropagtion of Actinidia cultivars

Reguladores de crescimento vegetal na micropropagação de cultivares de Actinidia

ABSTRACT