Physiological potential of piquin pepper seeds in response to pregermination treatments

Potencial fisiológico de sementes de pimenta “piquin” em resposta a tratamentos pré-germinativos

ABSTRACT

Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA₃, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H₂SO₄; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T₅₀), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T₅₀ and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

Index terms:
Capsicum annuum L. var. glabriusculum (Dunal) Heiser & Pickershill; growth regulators; imbibition; germination.

RESUMO

Os frutos silvestres da pimenta piquín são consumidos e comercializados, devido ao seu sabor e propriedades nutracêuticas. a germinação heterogênea das sementes fez com que os tratamentos pré-germinativos não fossem padronizados. Portanto, nosso objetivo foi avaliar tratamentos pré-germinativos de sementes de pimenta piquín de uma planta silvestre do município de Victoria, Tamaulipas, México, consistindo em imersão por diferentes períodos (2, 24 e 48 h) em soluções com concentrações variáveis dos seguintes hormônios vegetais: ácido naftaleno acético (NAA; 0,1, 1,0, 2,5 mM), ácido salicílico (SA; 1,0, 5,0, 10 mM), ácido giberélico (GA3, 1,0, 5, 0,10 mM), ácido indol-3-butírico (IBA; 1,0, 2,0, 3,0 mM), além do bioestimulante comercial Biozyme comercial (Bioz) contendo minerais e reguladores de crescimento (Bioz; 0,5, 1, 2%) e ácido sulfúrico (H₂SO₄; 2,5, 10%) em um delineamento inteiramente casualizado. usando água destilada como controle. Para cada tratamento, medimos o índice de embebição, a porcentagem de germinação, o tempo para obtenção de 50% de germinação (T50) e o coeficiente de variação do tempo de germinação (CVt). Nossos resultados mostraram que a embebição foi semelhante no controle e nos tratamentos, mas diferiu entre os tratamentos. A maior embebição registrada em 48 h foi de 87%. A maior porcentagem de germinação (79,2%) foi alcançada com 2 h de imersão em 2% Bioz, seguido por 1 mM NAA (62%) e 5 mM SA. T50 e CVt foram de 10-26 dias e 0-39%, respectivamente. De acordo com nossos resultados, concluímos que os tratamentos de pré-germinação que testamos melhoraram o potencial fisiológico para germinação de sementes de pimenta piquin.

Termos para indexação:
Capsicum annuum L. var. glabriusculum; regulador vegetal; embebição; germinação

INTRODUCTION

Capsicum annuum L. var. glabriusculum (Dunal) Heiser and Pickershill (piquin pepper) is a perennial plant in the Solanaceae family distributed from the southwestern U.S.A. to Colombia, which is considered to be the ancestor of all cultivated variants of chili peppers belonging to C. annuum (Hayano-Kanashiro; Gámez-Meza; Medina-Juárez, 2016). In their natural environment, wild piquin pepper plants associate with trees or shrubs that provide them shade, phytosanitary protection, and soil organic matter. In Mexico, local people harvest fruits from wild populations and cultivate plants for consumption and sale. Because of genetic resistance to the diseases and environmental stress factors present in their populations, piquin pepper has the potential for the genetic improvement of cultivated chilli peppers (Lin et al., 2021).

The fruits of the piquin pepper are consumed fresh (with a green pericarp), dried (red), and in processed products due to their organoleptic properties (pungency, taste, and smell) and low irritability to the digestive tract. Piquin pepper fruits also have phytochemical molecules with nutritional and pharmaceutical attributes, including phenolic compounds, capsaicinoids, carotenoids, and tocopherols of health importance (Hayano-Kanashiro; Gámez-Meza; Medina-Juárez, 2016; Treto-Alemán et al., 2021). The sale of fruits, gathered from wild plants or harvested from cultivated plants during the rainy season, contributes to the economy of rural populations, and their market value is up to 40 times that of the jalapeño or serrano varieties (Villalon-Mendoza et al., 2014). However, the harvesting methods applied cause mechanical damage to the plants, and in extreme cases, their removal, which compromises their regeneration, lowers their productivity and decreases the population’s genetic diversity (Medina-Martínez et al., 2010).

The germination rate of wild piquin pepper seeds is under 20%, which limits their domestication and commercialization as a crop (López-España et al., 2017; Quintero et al., 2018). This low germination rate is due to a variable degree of seed dormancy, which can be caused by several factors, including the mucilaginous cover in the seed coat (testa), which hardens when dry, a high concentration of germination-inhibiting compounds such as abscisic acid (ABA) or capsaicin, and rudimentary or physiologically immature embryos (Prado-Urbina et al., 2015; Barchenger; Bosland, 2016; Mares-Quiñones; Valiente-Banuet, 2019). Additionally, Herrera-Aguilar et al. (2018) reported a loss of viability after seeds were subjected to accelerated aging.

The proposed pregermination treatments for overcoming dormancy in Capsicum spp. seeds and making their germination response uniform include physical (hydrothermal, light) and chemical (KNO₃, strong acids such as H₂SO₄ and HNO₃, and growth regulators) agents or their combination. For treatment with growth regulators, seeds are imbibed by immersion in chemical solutions for a period of time, which triggers the activation of enzymes and growth regulators, starch hydrolysis, and nutrient transport favoring embryo growth and development (Louf et al., 2018). Growth regulators used for pregermination treatments include 5.70 mM indoleacetic acid (IAA), 0.04-0.4 mM kinetin, and 0.02-0.2 mM gibberellic acid (GA₃) (Watkins; Cantliffe, 1983). However, Mireles-Rodríguez et al. (2015) reported a variable germination response of piquin pepper seeds depending on their provenance in the state of Tamaulipas, Mexico: seeds collected in Ocampo and Jaumave had a better germinative and agronomic performance after treatment with 14.4 mM GA₃ than those collected in Chamal and San Carlos.

The genetic variability present in the wild populations of piquin pepper, expressed in the morphological characteristics of plants and fruits, has hampered the finding of a general pregermination treatment protocol. Despite the advancements made in the knowledge about the germination of chili pepper seeds, the imbibition behavior of seeds in conditioning solutions, the distribution of solutes in their tissues, and the response and action mechanisms of plant growth hormones remain unclear (Terskikh et al., 2011; Silva et al., 2020). For that reason, our objective in this study was to evaluate the effect of pregermination treatments based on growth regulators, sulfuric acid, and Biozyme solutions at different immersion periods on the imbibition and germination of piquin pepper seeds.

MATERIAL AND METHODS

Location of the experiment

The assays were carried out in the Plant Biotechnology Laboratory of the Engineering and Sciences Faculty of the Autonomous University of Tamaulipas in Ciudad Victoria, Tamaulipas, Mexico.

Processing of fruits and seed selection

Fruits were collected from a wild plant growing in a rural area in the community of Tierra Nueva (23° 50’ 28” N, 99° 7’ 39” W) in the municipality of Victoria, Tamaulipas. Mature fruits (selected for their red color) were picked and washed in 1% commercial dish detergent (Axion, Colgate-Palmolive Company) for 10 min, had their pericarp removed with a plastic spatula, and then were rinsed in sterile water to eliminate the remaining mucilage. The seeds that floated in the rinsing water were removed. The selected seeds were air dried at room temperature and stored for 30 days in Petri dishes at 25 °C and 50% relative humidity.

Pregermination treatment solutions

Pregermination imbibition solutions contained distilled water as the control treatment; 0.1, 1.0, and 2.5 mM NAA (Sigma-Aldrich); 1.0, 5.0, and 10 mM SA (Wako 196-14861); 1.0, 5.0, and 10 mM GA₃ (Biogib 10ps, GBM S.A. de C.V.); 1.0, 2.0, and 3.0 mM IBA (Bio Basic IB0725); 0.5, 1, and 2% Bioz (GBM S.A. de C.V.); and 2, 5, 10% sulfuric acid (H₂SO₄; Jalmek Científica S.A. de C.V.). The combination of products and their concentrations defined the treatments (Table 1).

Table 1:
Pregermination treatments applied to piquin pepper seeds.

Treatment number	[dist. H20] (%) Ctrl	[NAA] (mM)	[SA] (mM)	[GA3] (mM)	[IBA] (mM)	[Bioz] (%)	[H2SO4] (%)
1	100	-	-	-	-	-	-
2	-	0.1	-	-	-	-	-
3	-	1.0	-	-	-	-	-
4	-	2.5	-	-	-	-	-
5	-	-	1.0	-	-	-	-
6	-	-	5.0	-	-	-	-
7	-	-	10.0	-	-	-	-
8	-	-	-	1.0	-	-	-
9	-	-	-	5.0	-	-	-
10	-	-	-	10.0	-	-	-
11	-	-	-	-	1.0	-	-
12	-	-	-	-	2.0	-	-
13	-	-	-	-	3.0	-	-
14	-	-	-	-	-	0.5	-
15	-	-	-	-	-	1.0	-
16	-	-	-	-	-	2.0	-
17	-	-	-	-	-	-	2.0
18	-	-	-	-	-	-	5.0
19	-	-	-	-	-	-	10.0

• Abbreviations: dist. H₂0: Distilled water. Ctrl: Control. NAA: Naphthaleneacetic acid. SA: Salicylic acid. GA₃: Gibberellic acid. IBA: Indole-3-butyric acid. Bioz: Biozyme. H₂SO₄: Sulfuric acid.

Imbibition and germination assays

For the imbibition assays, the dry weight of the seeds was measured in an analytical balance (Nimbus 254e, Adam NBL), after which the seeds were placed in Petri dishes containing the treatment solutions and imbibed for 2, 24, and 48 h. Each treatment used 25 seeds in triplicate. The Petri dishes were placed in a culture room at 25 °C and a 16/8 h (light/darkness) photoperiod. After the imbibition period, the seeds were carefully removed from Petri dishes with tweezers, placed on paper towels to remove the excess solution, and weighed. Immediately after, they were reimmersed in the corresponding treatment solution.

The seeds used for germination assays were first disinfected by soaking them for 10 min in 70% (v/v) ethanol, rinsing them twice in distilled water for 10 min each time, soaking them in 10% (v/v) commercial hypochlorite solution for 10 min, and finally rinsing them twice in distilled water for 10 min each time. The germination assays were performed in triplicate using 50 disinfected seeds per treatment, and they were placed in Petri dishes containing the respective solution and imbibed for periods of 2, 24, and 48 h. After the imbibition period, the seeds were carefully removed from the Petri dishes with tweezers, rinsed in distilled water to remove the treatment solution, placed in Petri dishes lined with filter paper containing 5 mL of distilled water, and incubated in a culture room at a temperature of 25 °C and a 16/8 h (light/darkness) photoperiod for 30 days.

Recorded variables

Imbibition ratio of seeds

The amount of water imbibed by the seeds during the treatment period was calculated using the differences between the dry weight (Wi) and the weight of the imbibed seeds after the treatment (Wf). For each treatment and immersion period, the imbibition ratio of the seeds was calculated from the total weight gain of the seeds relative to their initial dry weight by the formula:

$WC=\frac{\left(Wf-Wi\right)}{Wi}x100$

where:

WC = seed water content

Wi = initial dry weight of seeds

Wf = final weight of seeds

Germination percentage

The percentages of seed germination were obtained by counting the seeds showing radicle emergence every three days for a total of 30 days and then calculating the percentages.

Time to obtain 50% germination (T₅₀)

The time needed to obtain 50% germination (T₅₀) was calculated by the Coolbear, Francis and Grierson (1984) modified by Farooq et al. (2005) equation:

$T_{50}=t_i+\frac{\left(\frac{N}{2}-n_i\right)\left(t_j-t_i\right)}{n_j-n_i}$

where:

n_i = number of germinated seeds before reaching 50% of germinated seeds

n_j = number of germinated seeds after reaching 50% of germinated seeds

t_i = time in days before reaching 50% germinated seeds

t_j = time in days after reaching 50% of germinated seeds

N = total number of germinated seeds

Coefficient of variation of germination time (CVt)

To obtain information about the variation in germination time in days, the coefficient of variation of germination time (CVt) was calculated following Carvalho, Santana and Ranal (2005) by:

$Vt=\frac{St}{\overline{t}};St=\sqrt{\frac{\sum n_i{\left(t_i-\overline{t}\right)}^2}{\sum n_i-1}};\overline{t}=\frac{\sum n_i{t}_i}{\sum n_i}$

where:

St = variance of germination time

= mean germination time in days

n_i = number of seeds germinated in day i

t_i = number of days after sowing

Statistical analyses

The imbibition and germination experiments were performed under a totally randomized experimental model. The germination and imbibition percentages were transformed using the arcsine square root, and the obtained data were statistically analyzed by ANOVA and the Tukey (p ≤ 0.05) mean comparison test in Statgraphics Centurion XVI version 16.1.03.

RESULTS AND DISCUSSION

Seed imbibition

We observed a gradual increase in the imbibition ratio in response to the applied pregermination treatments relative to the control. At 2, 24, and 48 h, the corresponding germination values were 71, 84, and 87%, respectively. After 2 h of imbibition, we observed statistically significant differences (p ≤ 0.05) within and between the GA₃ (10 mM), NAA (0.1 mM), SA (1 mM), Bioz (1%), and H₂SO₄ (5 and 10%) treatments. The highest percent imbibition (81.8%) occurred in the 10 mM GA₃ treatment, and the seed imbibition percentages of seeds ranged between 61.1 and 81.8% (Figure 1A).

Figure 1:
Percent imbibition of piquin pepper seeds in pregermination treatments at 2 h (A), 24 h (B), and 48 h (C) immersion periods in distilled water (Ctrl), naphthaleneacetic acid (NAA), salicylic acid (SA), gibberellic acid (GA₃), indolebutyric acid (IBA), Biozyme (Bioz), and sulfuric acid (H₂SO₄). The values shown in each bar represent the mean (n=3). Different letters indicate statistically significant differences between treatments (Tukey, p ≤ 0.05).

At 24 h of imbibition, the weight of the seeds changed from 69.1 to 98.3%, which shows a trend similar to that we observed after 2 h of imbibition, but at a slightly slower seed hydration rate (Figure 1B). After 48 h, the imbibition increased to between 73 and 97%; however, immersion of seeds in GA₃ (10 mM) caused a 1% decrease in their weight. The imbibition percentages of seeds immersed in GA₃, NAA, IBA, Bioz, SA, and H₂SO₄ solutions were not significantly different from that in the control, regardless of the imbibition period (Figure 1A-C).

Several studies have noticed that the seed coat, in addition to protecting the embryo and reserve tissues, also plays a role in the regulation of germination by preventing the entrance of water (Smýkal et al., 2014). Thus, the seed coat represents a physical dormancy mechanism that hampers the germination of seeds of species in the Solanaceae family, including those in the Capsicum genus. Since in all treatments we observed that the imbibition began at 2 h of immersion, we concluded that the seed coat is not a barrier to imbibition that would cause a physical dormancy of piquin pepper seeds and that dormancy in the piquin pepper seeds must be associated with other factors. The time of imbibition depends on seed characteristics such as the amount of tissue to be hydrated, that is, seed weight and size, initial water content, and water availability (Louf et al., 2018). The same response was observed by similar studies in the Capsicum genus. Espitia-Hernández et al. (2019) evaluated the germination-inhibiting effects of different concentrations of growth regulators and potassium nitrate in chile ancho pepper seeds (Capsicum annuum) and found no significant differences in the treatments relative to the control. Garruña-Hernández et al. (2014) reported a similar absence of significant differences in the imbibition of Habanero pepper (C. chinense Jacq.) seeds after 12, 36, and 60 h immersion in ABA and G₃ relative to the control. In contrast, Cano-Vázquez et al. (2015) found a 70% increase in the water content of piquin pepper seeds (collected in Tuxpan, Veracruz) after 8 h of imbibition. Monroy-Vázquez et al. (2017) also observed that the imbibition of seeds from four species of Opuntia took place during the first hours of immersion.

The rapid imbibition during the first 2 h of immersion that we observed in piquin pepper seeds, and its continued increase after 48 h, is related to the wild nature of the mother plants; that is, it suggests that the presence of morphophysiological characteristics (such as testa thickness, osmolytes, and osmotic potential) allows seeds to take maximum advantage of the variable episodes of soil water availability from rainfall, therefore improving the germination and generational succession of the species’ populations. In a similar context, Rodríguez-Morales, Guillén and Casas (2013) reported that wild seeds of Stenocereus stellatus in a hydric-stress gradient had higher germination percentages than managed populations.

Imbibition is the initial phase of the germination process; however, dormant seeds may express other factors, such as the synthesis of inhibitors that arrest the subsequent phases of the process (Weitbrecht; Müller; Leubner-Metzger, 2011). Because imbibition is driven by differences in the water potential, inviable seeds might also absorb water (Smýkal et al., 2014), as could have occurred in our 10% H₂SO₄ treatment after 48 h, in which we observed a similar seed weight gain as in the control, but with differences in seed germination percentage (Figure 2C).

Figure 2:
Percent germination of piquin pepper seeds in pregermination treatments at 2 h (A), 24 h (B), and 48 h (C) immersion periods in distilled water (Ctrl), naphthaleneacetic acid (NAA), salicylic acid (SA), gibberellic acid (GA₃), indolebutyric acid (IBA), Biozyme (Bioz), and sulfuric acid (H₂SO₄). The values shown in each bar represent the mean (n=3). Different letters indicate statistically significant differences between treatments (Tukey, p ≤0.05).

Seed germination

The diverse treatments applied by agronomists for homogenizing the germination response of seeds are aimed at overcoming the problem of uneven germination that limits the establishment of crops. The results from our analysis of variance showed significant differences (p≤ 0.05) in germination response between treatment solutions and immersion times. Treatment with 2% Bioz promoted the highest germination response (79.3%) regardless of the immersion period (Figure 2). González-Cortés et al. (2015) reported a similar response in wild chili pepper seeds collected in the state of Tabasco, Mexico, immersed in 1.6% Bioz for 24 h, which increased their germination by 40% relative to the control. The authors of the study attributed this increase in germination response to the influence of the product’s chemical content (minerals, GA₃, auxin, and cytokines) on the biochemical, metabolic, and physiological processes taking place during seed germination (Carrera-Castaño et al., 2020).

In our study, treatment of piquin pepper seeds with the growth regulators NAA and IBA improved their germination. The germination percentage of seeds immersed for 2 h in 1.0 mM NAA was 62%, and it was 54.6% after 24 h in 0.1 mM NAA; after 48 h in 0.1 and 1.0 mM NAA, it was 37 and 42%, respectively. These results agree with those of Quintana et al. (2013), who treated seeds of Clitoria ternatea L. conserved for 20 years in a seed bank with NAA, AG₃, and NAA+AG₃, finding an 18% germination percentage increase with 0.5 µM NAA relative to the control.

We observed that immersion in 1.0 mM IBA produced the highest germination percentages after 24 h (56.6%) and 48 h (41.3%) (Figure 2). The treatment with higher concentrations of IBA produced lower germination percentages than the control at all immersion periods. Similarly, in a study with Passiflora quadrangularis L. seeds, Carranza et al. (2016) obtained a 3% increase in germination after 13 days when treating seeds with 1-2.95 mM IBA relative to the control treatment. Guangwu and Xuwen (2014) evaluated the germination response of two varieties of Pinus massoniana Lamb. (Dai 1 and Dai 2) treated with IAA and GA₃, finding increased germination in both treatments; however, the increase with IAA depended on the variety (33% in Dai 1 and 27% in Dai 2), which suggests the involvement of IAA and GA₃ in the feedback mechanisms regulating seed germination.

Immersion in GA₃, at all the concentrations and immersion periods we tested, did not improve the germination of piquin pepper seeds, although treatment with 10 mM GA₃ for 24 h resulted in a slight tendency to improve it. Our results in this regard differ from those of Prado-Urbina et al. (2015), who reported a 30 to 40% increase in the germination of wild accessions of C. annuum seeds from Tabasco when the seeds were previously immersed in 14.43 mM GA₃. Similarly, after treatment with 0.577 mM GA₃, Quintero et al. (2018) observed a 70% increase in the germination of piquin pepper seeds collected in Tamaulipas and San Luis Potosí, Mexico. We know that the essential function of gibberellins in germination is mediated by their equilibrium with abscisic acid (Miransari; Smith, 2014; Hu et al., 2021) and that gibberellins contribute to overcoming dormancy and nourishing the embryo through the activity of the hydrolytic enzyme α-amylase. Therefore, because imbibition was not the limiting factor (Figure 1), it is possible that the low germination percentages that we observed in treatments with GA₃ might have been due to the hormone’s effect on α-amylase at the concentrations and immersion periods that we assayed. Other endogenous factors known to control germination are plant hormone activities, seed provenance, present population variation, and seed viability (Cano-Vázquez et al., 2015).

The imbibition of seeds with SA had effects on their germination under all treatment conditions. The highest germination percentage that we observed was 56%, when seeds were immersed in 5 mM SA for 2 h, while at 1.0 mM for 24 h, it was 47.3%. The differences between seeds immersed in SA for 24 h and the control were not statistically significant (Figure 2). Ahmed et al. (2020) had similar germination improvement results with sweet pepper cv. Yolo seeds immersed in SA (0.2 and 0.3 mM), 33% of which germinated. SA is a plant hormone involved in growth regulation and plant development that also is associated with tolerance to several biotic and abiotic stress factors and seed germination (Liu et al., 2019). The induction of malate dehydrogenase activity by SA is involved in the germination of Arabidopsis thaliana (L.) Heynh. and other plants, with high levels of the enzyme correlating with the beginning of germination (Weitbrecht et al., 2011). Stress from NaCl during the germination of Limonium bicolor (Binge) Kuntz seeds is modulated by SA through the stimulation of endogenous GA₃ and α-amilasa and the inhibition of ABA (Liu et al., 2019). The metabolic complexity of the seed germination process is made evident by the communication between biosynthetic pathways (Weitbrecht et al., 2011).

The imbibition of seeds in 2% H₂SO₄ for 2 h increased their germination by 103%, but after 2 h of immersion at all concentrations, germination was reduced by 85% relative to the control (Figure 2A). H₂SO₄ is one of the acids most commonly used for the scarification of seeds to allow their imbibition. The decrease in germination percentage that we observed might have been due to cellular damage caused by prolonged immersion in the acid. Thus, the selection of chemical seed scarification treatments must consider the species, concentration, and immersion time. In that regard, Merino-Valdés et al. (2018) observed the destruction of Capsicum pubescens Ruiz & Pav. seeds immersed for 30 min in 100, 75, and 65% H₂SO₄ solutions.

Time to obtain 50% germination (T₅₀)

The time to obtain 50% germination (T₅₀) was statistically different (p≤0.05) among the immersion treatments (Figure 3), and it was reached between 10 and 20 days. In the first 2 h of imbibition in NAA (0.1 mM), SA (10 mM), GA₃ (5 and 10 mM), Bioz (1.0%), and H₂SO₄ (5%), the T₅₀ values were longer than 20 d (22.2, 22.3, 25.1, 26.3, 25.2, and 22.2 d, respectively). Seed immersion for 24 h in 10 mM GA₃ and for 48 h in 1.0 mM GA₃ were the only treatments in which T₅₀ was above 20 (22.75 and 27.5 d, respectively). The remaining treatments did not show significant differences relative to the control. The lowest T₅₀ value that we recorded, which correlated with a higher germination percentage, was that for 2% Bioz (79.3% germination and 11 d T₅₀), followed by 1.0 mM NAA (62.0%, 14 d) and 5 mM SA (56.0%, 15 d). The reduction in the T₅₀ value that we observed could have been due to treatments causing the rapid and irreversible initiation of the metabolic processes associated with germination once seeds are imbibed (Louf et al., 2018).

Figure 3:
Time in days to obtain 50% germination (T₅₀) of piquin pepper seeds in pregermination treatments at 2 h (A), 24 h (B), and 48 h (C) immersion periods in distilled water (Ctrl), naphthaleneacetic acid (NAA), salicylic acid (SA), gibberellic acid (GA₃), indolebutyric acid (IBA), Biozyme (Bioz), and sulfuric acid (H₂SO₄). The values shown in each bar represent the mean (n=3). Different letters indicate statistically significant differences between treatments (Tukey, p ≤0.05).

Variations in T₅₀ can be caused by treatments and seed provenance (ecotype), as reported for piquin pepper seeds collected in northwestern Mexico by López-España et al. (2017), who observed a T₅₀ of 26.39 d under variable temperature and of 57.7 d at constant temperature (the authors found the lowest T₅₀ when sunlight was reduced by 50%), and by Quintero et al. (2018), who recorded a T₅₀ of 8.1 d, representing a reduction of 16 d relative to the control, with a treatment combining KNO₃ and GA₃.

Coefficient of variation of germination time (CVt)

The CVt values we found did not show statistically significant differences among treatments relative to the control, except for 2 mM IBA, 1 and 10 mM GA₃, and 10% H₂SO₄, in which the CVt values were less than 2. These low CVt values were due to the limited germination occurring during a short period. The highest CVt (39.6) and germination values (79.2%) and the lowest T₅₀ value (11 d) that we observed corresponded to the seeds treated with 2% Bioz, followed by NAA and SA (Figure 4).

The variability in germination response that we observed in the piquin pepper seeds might have ecological, genetic, and metabolic causes. According to Mendes-Rodrigues, Oliveira and Ranal (2011), the asynchrony and heterogeneity of germination responses in seeds of wild plant species increase their capability to survive. Thus, the strategy followed by wild plant species such as piquin pepper is to disperse seeds with different degrees of dormancy to reduce the risk of germination in an unfavorable environment, while the mechanisms of dormancy interruption involve environmental factors, the plant’s genetic profile, and the endogenous levels of hormonal activity (Smýkal et al., 2014; Carrera-Castaño et al., 2020).

Figure 4:
Coefficient of variation of germination time (CVt) of piquin pepper seeds in pregermination treatments at 2 h (A), 24 h (B), and 48 h (C) immersion periods in distilled water (Ctrl), naphthaleneacetic acid (NAA), salicylic acid (SA), gibberellic acid (GA₃), indolebutyric acid (IBA), Biozyme (Bioz), and sulfuric acid (H₂SO₄). The values shown in each bar represent the mean (n=3). Different letters indicate statistically significant differences between treatments (Tukey, p ≤0.05).

CONCLUSIONS

According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds by modifying T₅₀ and CVt. Because water imbibition was high during the first hours of immersion, we demonstrated that piquin pepper seeds lack physical dormancy. Among the pregermination treatments that we applied, the best response occurred after imbibition in 2% Bioz for 2 h, followed by those in treatments with 1 mM NAA and 5 mM SA.

AUTHOR CONTRIBUTIONS

Conceptual idea: Poot-Poot, W.A.; Methodology design: Poot-Poot, W.A.; Cano-González, M.A.; Ayi-Gutiérrez, B.A.; Data collection: Cano-González, M.A.; Osorio-Hernández, E.; Ayi-Gutiérrez, B.A.; Data analysis and interpretation: Rangel-Lucio, J.A.; Delgado-Martínez, R.; Poot-Poot, W.A.; and Writing and editing: Cano-González, M.A.; Osorio-Hernández, E.; Poot-Poot, W.A.; Rangel-Lucio, J.A.

ACKNOWLEDGMENTS

The main author acknowledges the Consejo Nacional de Ciencia y Tecnología (CONACYT) for granting his doctoral scholarship (No. 749126) and the Faculty of Engineering and Sciences of the Autonomous University of Tamaulipas (UAT) for providing their facilities during this research.

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Publication Dates

• Publication in this collection
  08 Dec 2021
• Date of issue
  2021

History

• Received
  06 Sept 2021
• Accepted
  05 Nov 2021