Microencapsulação de NCFM de <i>Lactobacillus acidophilus</i> com manitol e sua estabilidade de armazenamento em chá de amoreira

Lactobacillus acidophilus NCFM (L-NCFM) foi microencapsulado pela técnica de coextrusão com manitol. A otimização do material de revestimento, goma de alfarroba (0% a 2%, p / v) e prebiótico, manitol (0% a 5%, p / v) foi testada no tamanho do grânulo e eficiência de microencapsulação. Células L-NCFM microencapsuladas em três formas diferentes foram testadas em suco gástrico simulado (pH 2,0) e suco intestinal simulado (pH 7,5) e teste de armazenamento a 4 °C e 25 °C por 4 semanas. 0,5% (p / v) de goma de alfarroba e 3% (p / v) de manitol são as concentrações óptimas para produzir um tamanho de pérola de 570 µm, eficiência de microencapsulação de 96,81% e contagem de células 8,92 log CFU / mL. A microencapsulação de L-NCFM com manitol protege melhor as células em ambiente ácido. A viabilidade de L-NCFM encapsulado com manitol a 4 °C durante o período de armazenamento por 30 dias variou de 8,62 log ufc / mL a 6,80 log ufc / mL, atendendo ao mínimo necessário para probiótico (10⁶ UFC / mL).

Termos para indexação:
Probiótico; co-extrusão; manitol; Lactobacillus.

Autoria

Wong Lok Yee

UCSI University, Faculty of Applied Sciences, Department of Food Science with Nutrition, Kuala Lumpur, MalaysiaUCSI UniversityMalaysiaKuala Lumpur, MalaysiaUCSI University, Faculty of Applied Sciences, Department of Food Science with Nutrition, Kuala Lumpur, Malaysia

http://orcid.org/0000-0002-4035-5346

Chan Li Yee

http://orcid.org/0000-0001-9781-0728

Nyam Kar Lin

http://orcid.org/0000-0002-2410-9823

Pui Liew Phing ^**Corresponding author: puilp@ucsiuniversity.edu.my

http://orcid.org/0000-0001-5305-4334

^*Corresponding author: puilp@ucsiuniversity.edu.my

SCIMAGO INSTITUTIONS RANKINGS

Figuras | Tabelas | Fórmulas

Figuras (2)
Tabelas (4)
Fórmulas (3)

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Figure 1:
Size and shape of beads measured with a scale micrometer.

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Figure 2:
Survival of free cell, encapsulated Lactobacillus acidophilus NCFM with and without mannitol in mulberry tea over 4 weeks of storage at refrigerator (4 °C) and room temperature (25 °C).

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Table 1:
Effect of different concentration of locust bean gum on bead size and microencapsulation efficiency of locust bean gum coated microencapsulated L-NCFM.

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Table 2:
Effect of different concentration of mannitol on average log CFU/mL, bead size and microencapsulation efficiency of locust bean gum coated microencapsulated L-NCFM.

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Table 3:
Average log₁₀ CFU/mL, bead size in diameter and microencapsulation efficiency of L-NCFM.

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Table 4:
Average log₁₀ CFU/mL and survivability of free cell, encapsulated L-NCFM with and without mannitol under sequential digestion.

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(1)

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(2)

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(3)

Figure 1: Size and shape of beads measured with a scale micrometer.

Figure 2: Survival of free cell, encapsulated Lactobacillus acidophilus NCFM with and without mannitol in mulberry tea over 4 weeks of storage at refrigerator (4 °C) and room temperature (25 °C).

Table 1: Effect of different concentration of locust bean gum on bead size and microencapsulation efficiency of locust bean gum coated microencapsulated L-NCFM.

Locust bean gum (%, w/v)	Diameter (µm)	Microencapsulation efficiency (%)
0.0	550.00 ± 55.68^b	93.70 ± 0.79^a
0.5	576.67 ± 47.26^ab	94.70 ± 0.62^a
1.0	613.33 ± 15.28^ab	90.27 ± 0.21^b
1.5	673.33 ± 25.17^a	89.21 ± 0.68^b
2.0	623.33 ± 28.87^ab	93.34 ± 0.81^a

^a-bMeans ± standard deviations followed by different superscript letters within the same column are significantly different at p < 0.05 according to Tukey’s test.

Table 2: Effect of different concentration of mannitol on average log CFU/mL, bead size and microencapsulation efficiency of locust bean gum coated microencapsulated L-NCFM.

Mannitol (%, w/v)	Average Log₁₀ CFU/mL	Diameter (μm)	Microencapsulation efficiency (%)
0.0	7.54±0.17^d	543.33±30.55^b	95.88±1.18^a
1.0	7.41±0.08^d	576.67±25.17^b	85.86±1.34^c
2.0	7.47±0.02^d	613.33±35.12^ab	91.92±0.78^b
3.0	8.92±0.08^a	570.00±50.00^b	96.81±0.86^a
4.0	7.92±0.11^c	670.00±20.00^a	94.37±0.62^ab
5.0	8.39±0.16^b	610.00±26.46^ab	93.57±0.89^ab

^a-dMeans ± standard deviations followed by different superscript letters within the same column are significantly different at p < 0.05 according to Tukey’s test.

Table 3: Average log₁₀ CFU/mL, bead size in diameter and microencapsulation efficiency of L-NCFM.

Probiotic	Prebiotic	Coating material	Average log₁₀ CFU/mL	Diameter (μm)	Microencapsulation efficiency (%)
L-NCFM	-	-	7.54±0.17^b	550.00±55.68^a	93.70±0.79^b
L-NCFM	-	Locust bean gum	7.54±0.17^b	576.67±47.26^a	95.88±1.28^ab
L-NCFM	Mannitol	Locust bean gum	8.92±0.08^a	570.00±50.00^a	96.81±0.86^a

^a-bMeans ± standard deviations followed by different superscript letters within the same column are significantly different at p < 0.05 according to Tukey’s test. S₁: without mannitol without locust bean gum; S₂: without mannitol with locust bean gum; S₃: with mannitol with locust bean gum.

Table 4: Average log₁₀ CFU/mL and survivability of free cell, encapsulated L-NCFM with and without mannitol under sequential digestion.

Sequential incubation	Time (h)	Average log₁₀ CFU/mL Survivability (%)
Sequential incubation	Time (h)	Free cell	Encapsulated L-NCFM	Encapsulated L-NCFM with mannitol	Free cell	Encapsulated L-NCFM	Encapsulated L-NCFM with mannitol
SGJ (pH 2.0)	0	8.64±0.07^Aa	8.46±0.04^Ab	8.57±0.05^Aab	100.00±0.00^Aa	100.00±0.00^Aa	100.00±0.00^Aa
	1	6.67±0.03^Bc	7.33±0.02^Bb	7.71±0.02^Ba	77.17±0.75^Bc	86.66±0.19^Bb	89.89±0.68^Ba
	2	5.61±0.04^Cc	6.47±0.05^Cb	6.72±0.03^Ca	64.91±0.76^Cc	76.52±0.48^Cb	78.38±0.44^Ca
SIJ (pH 7.5)	3	3.49±0.03^Dc	5.78±0.10^Db	6.35±0.01^Da	40.38±0.47^Dc	68.30±0.95^Db	74.08±0.30^Da
SIJ (pH 7.5)	4	0.00±0.00^Ec	5.68±0.04^Db	6.21±0.04^Da	0.00±0.00^Ec	67.21±0.19^Db	72.35±0.85^Da
	5	0.00±0.00^Ec	3.62±0.03^Eb	4.72 ±0.03^Ea	0.00±0.00^Ec	42.84±0.24^Eb	55.03 ±0.61^Ea
	6	0.00±0.00^Ea	0.00±0.00^Fa	0.00±0.00^Fa	0.00±0.00^Ea	0.00±0.00^Fa	0.00±0.00^Fa

^A-F Means ± standard deviations followed by different superscript letters within the same column are significantly different at p < 0.05 according to Tukey’s test. ^a-c Means ± standard deviations followed by different superscript letters within the same row are significantly different at p < 0.05 according to Tukey’s test.

(1)

Colony forming unit (CFU/mL) = \frac{Average number of colonies}{Dilution factor \times volume plated}

(2)

Microencapsulation efficiency (%) {= (Log}_{10} {N/Log}_{10} N_{0}) x 100

(3)

Survivability (%) {= Log}_{10} N_{t} {/ Log}_{10} N_{0} x 100

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Microencapsulação de NCFM de <i>Lactobacillus acidophilus</i> com manitol e sua estabilidade de armazenamento em chá de amoreira

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[1] ^*Corresponding author: puilp@ucsiuniversity.edu.my