Allele dosage of PVY resistance genes in potato clones using molecular markers

Kneib, Raquel Bartz; Kneib, Roberta Bartz; Pereira, Arione da Silva; Castro, Caroline Marques

doi:10.1590/1984-70332017v17n4a47

Acessibilidade / Reportar erro

Brasil

Crop Breeding and Applied Biotechnology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

ARTICLE • Crop Breed. Appl. Biotechnol. 17 (4) • Dec 2017 • https://doi.org/10.1590/1984-70332017v17n4a47 copy

Allele dosage of PVY resistance genes in potato clones using molecular markers

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Potato virus Y (PVY) is considered one of the most important pathogens in potato crop. The objective of this study was to estimate the allele dosage of Ry genes and select clones with multiple copies of resistance alleles to be used as parents in potato breeding programs. Seven clonal families were genotyped, including five parents with resistance to PVY. For the allele Ry_adg, based on the marker RYSC3, four parents are simplex, and one is duplex. For the allele Ry_sto, based on the marker M45, two parents are simplex and one is duplex. For two parents, the observed frequencies of the allele Ry_sto did not statistically adjust to any of the proposed genotypic constitutions. Based on the results obtained, both markers must be used in marker assisted selection for resistance to PVY, in order to decrease the error of discarding clones with only one of the alleles.

Key words:
Solanum tuberosum L.; genetic improvement; Potato virus Y; RYSC3; M45

			RYSC3		Clones with the presence of the gene Ry _adg (%)	M45		Clones with the presence of the gene Ry _sto (%)
Family	Male x female parent	Total	+	-		+	-
C-1	BRS Clara x C1883-22-97*	105	36	69	34	29	76	28
C-2	BRS Ana x C1883-22-97*	111	38	73	34	36	75	32
C-3	BRS Ana x C2372-02-02*	114	39	75	34	27	87	24
C-4	Caesar x C2372-02-02*	104	40	64	38	34	70	33
C-5	C2388-01-02* x Asterix	68	35	33	51	33	35	49
C-6	C2389-01-02* x Asterix	100	74	26	74	71	29	71
C-7	MB9846-1* x 5897-1	109	47	62	43	49	60	45
Total		711	309	402	49	279	432	39

Families	Genetic contitution
Families	Nuliplex	Simplex	Duplex	Triplex	Quadruplex
C-1	129552.69**	5.88	117.57**	1064.09**	476052.69**
C-2	144350.02**	6.27	124.58**	1246.58**	532850.02**
C-3	152048.69**	6.47	128.09**	1158.11**	56244.69**
C-4	159950.77**	2.43	95.30**	916.32**	409550.77**
C-5	122466.03**	0.79	28.10**	359.13**	108866.03**
C-6	547561.52**	31.37**	0.97	129.38**	67561.53**
C-7	220846.54**	0.39	77.77**	811.38**	384346.53**

Families	Genetic Constitution
Families	Nuliplex	Simplex	Duplex	Triplex	Quadruplex
C-1	84058.02**		14.41**	156.1**	1306.08**	577558.02**
C-2	129551.36**		8.32**	135.04**	1320.03**	562451.35**
C-3	72858.80**		23.02**	196.95**	1585.72**	756858.79**
C-4	115554.24**		7.51**	125.08**	1109.01**	489954.23**
C-5	108866.03**		0.16	34.66**	408.04**	122466.03**
C-6	504058.82**		24.99**	2.92	166.92**	84058.83**
C-7	240046.06**		0.06	69.82**	838.61**	359946.06**

Crop Breeding and Applied Biotechnology Universidade Federal de Viçosa, Departamento de Fitotecnia, 36570-000 Viçosa - Minas Gerais/Brasil, Tel.: (55 31)3899-2611, Fax: (55 31)3899-2611 - Viçosa - MG - Brazil
E-mail: cbab@ufv.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] E-mail: raquelkneib@yahoo.com.br