Genetic improvement of sugar cane for bioenergy: the Brazilian experience in network research with RIDESA

– In this paper, it is presented RIDESA’s model for sugar cane breeding to ethanol, and its scientific, technological and hu man resources training contributions. RIDESA is an inter-university network for the development of sugar cane industry in Brazil, and was formed by a technical cooperation agreement between ten public universities. The model of network management is presented in this study, which involves, among other things, the public-private partnership (Universities-Mills) for the development of cultivars. RIDESA has produced 59 cultivars since 1990 and is now responsible for 59% of the total area cultivated with this plant in Brazil. In the last five years, 286 agronomists were trained in breeding programs at universities that comprise RIDESA. In this same period, the network formed 35 professors, 24 doctors and 7 post-docs in researches with this crop. It is also presented a conceptual approach on methods of sugar cane breeding involving families and genome-wide selection.


INTRODUCTION
Undoubtedly, the cultivars are the bases of the entire production chain involving the production of renewable energy, especially ethanol and bioelectricity, produced by sugar cane in Brazil.Therefore, it is crucial to maintain and increase investments in breeding, as well as in other technologies, so the sugar cane, among other species with potential energy, is competitive against other energy crops of worldwide interest.
However, when analyzing the background of sugar cane (Figure 1), it can be seen some instability in actions related to genetic improvement of this crop in Brazil.The first fact was the extinction, in 1990, of PLANALSUCAR (National Program for Sugar Cane Breeding).As a result, the breeding program was transferred to some public universities, which created RIDESA (Inter-University Network for the Development of Sugarcane Industry; www.ridesa.com.br),presented in more details in this review paper.The second action was the closing, in 2004, of the breeding program of COPERSUCAR (Central Cooperative of Sugar and Alcohol Producers of the State of São Paulo).Even with the law of cultivar protection, created in 1997 (Figure 1), COPERSUCAR had difficulties in collecting royalties for maintaining its breeding program, supported maintained by a group of about thirty cooperative mills since 1969.In 2004, COPERSUCAR became OSCIP (Civil Society Organization of Public Interest), denominated CTC (Sugarcane Technology Center), and in 2011, it became a Corporation, receiving financial support of hundreds of mills and suppliers.These changes were intended to attract more financial and technological resources for researches, in order to maintain its competitiveness and to ensure to Brazil the leadership position in the global sugar cane industry.However, such actions affected, differently and financially, both programs, which spent time and resources to adjust to the new reality.Historically, the financial resources invested in research and genetic improvement of sugarcane have been inconsistent and inadequate, compared to other species.

ARTICLE
MHP Barbosa et al.It is estimated that investments of 50 million dollars are required to develop a cultivar.Currently, the cost of cultivation of sugar cane, as a percentage of the cost of production, is much lower than in other crops.This cost has varied around 1-6 dollars per hectare, which represents less than 0.3% of the production cost.This is a very low value compared with other cultures.In the case of corn, for example, the germplasm cost is 10% of the production cost.For genetically modified corn and soybeans, the germplasm cost is around 15% and 10%, respectively.Therefore, for Brazil to remain competitive in the energy and sugar industry, from sugarcane cultivation, it is required additional public and private investments in classical and advanced genetics, as well as basic research and technological innovation throughout the production chain.
Since the first major investment of public resources during the implementation of Proálcool (National Ethanol Program) in 1974, only in 2006 the Brazilian government became interested again in the subject, when creating Embrapa Agroenergy and encouraging researchers from universities and institutes to do bioenergy research projects supported by their agencies.However such funds have been inconsistent and this will directly affect the competitiveness of the sugar cane in Brazil compared to other world scenarios.
However, even before this changing scenario, the total recoverable sugar productivity (TRS; sugars: sucrose, fructose and glucose) per hectare in Brazil has increased at the rate of 155.7 kg ha -1 ( Y = 3697.6+155.7x;R 2 = 0.93 ).In 1970, the national yield by hectare of TRS was 3712 and, in 2011, it reached  1975, 1985, 1995, 2005 and 2011; and the major events that impacted sugar cane industry after Proálcool started.In parentheses are shown the percentages of the main cultivars and the main producing states in 1975, 1985, 1995, 2005 and 2011.9148 kg ha -1 .Obviously, these gains are due to the use of technology, both in agriculture and in industry.In this field, cultivars occupied a prominent position, although it is difficult to quantify the contribution of each factor of production to the advance of this sector.
An estimate of the genetic improvement contributions considers that 50% of the productivity gain is due to the continuous replacement by other more productive cultivars.Thus, if considered half the advances of 155.7 kg ha -1 TRS, from 1970 to 2011, there would be 77.85 kg ha -1 .That would mean an increase of 700.6 million tons of TRS, in 2011, in an area of 9 million hectares in Brazil, due to the continuous replacement of cultivars.This would represent about 175 million dolar, considering the current price of TRS (U$0.24).In percentage terms, this represents an increase of 4% per year in terms of kg ha -1 of TRS, quite considerable when compared to the percentage gains reported in other cultures.Dias (2011) affirms that the Brazilian programs for sugar cane breeding has dropped 111 sugarcane cultivars, as registered by the Ministry of Agriculture, and that only in the last decade (2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009), new cultivars accounted for 20.8 % gain in productivity (in m 3 ha -1 ) with ethanol.

THE ORIGIN OF RIDESA
The IAA (Institute of Sugar and Alcohol), associated to the Ministry of Industry and Trade of the Federal Government, was established in 1933.This Institute had as main objectives the sugar market regulations in the country and the promotion of ethanol production.During the implementation of Proálcool, PLANALSUCAR was created on July 29 th , 1971, to power the development of technologies for the sugar cane sector.The program had five major regional coordinators, covering all Brazilian states producers, supported by regional experiment stations, located strategically throughout the country (Figure 2a).At that time, the program was funded exclusively by the federal government and had the support of several mills and distilleries to carry out experiments and validation of technological innovations, among them, the RB cultivars, i.e., Republic of Brazil.
Until 1988, the production of ethanol in Brazil was heavily regulated and subsidized by the Federal Government.With the promulgation of the new federal constitution in 1988, it was established a new scenario for economic policy, which ended with the subsidy programs, including Proálcool (Figure 1).It is important to emphasize that, during that period, the country was going through problems in its economy, like high inflation, besides the low price of oil (around US$ 25), which negatively and indirectly affected Proálcool.Therefore, the scenario was not favorable to the production of ethanol.Soon, all incentives to develop research with sugar cane, including breeding, were interrupted with the extinction of IAA in 1990.
Consequently, staff and infrastructure of coordinating and experimental stations of PLANALSUCAR were incorporated into some public universities, which belong now to RIDESA.Researchers and technicians were incorporated into universities, but without the financial resources to conduct their research with sugar cane.Those who remained in universities organized themselves and started the first projects for fundraising, especially near the mills and distilleries that produce sugar and ethanol.Therefore, it was necessary to split into areas of performance (Figure 2b), so that the financial resources of the private sector were distributed and invested in universities in order to promote research and to continue the program, especially breeding.But this transition was not easy, since without funds some universities had a lot of difficulty to enable their breeding programs.This occurred especially in those states with fewer sugar and ethanol mills.After this phase, RIDESA expanded with the incorporation of the Federal University of Paraná (UFPR) and Sergipe (UFS) in 1992.In 2003, the Federal University of Goiás (UFG) also joined RIDESA.The Federal Universities of Mato Grosso (UFMT) and Piauí (UFPI) became members in 2008.
However, since most sugar and ethanol companies were located in São Paulo (Figure 1), the Federal University of São Carlos (UFSCar) received a lot of financial contributions from mills and distilleries.As a result of infrastructure and germplasm that UFSCar got from PLANALSUCAR, five cultivars were released in 1992, another five in 1995, and six in 1998 (Table 1).Clones that originated these cultivars were created by PLANALSUCAR.It is observed that these cultivars have two digits after the code RB and are numbered less than 90.For example, the cultivar RB835486 had the seed produced in 1983 by PLANALSUCAR, and was incorporated by UFSCar, which finalized the experiments in RIDESA.The further code represents the selection number allocated to the respective clone, i.e. 5486.
Through the germplasm received from PLANALSUL-CAR by the Federal University of Alagoas (UFAL), it was released nine cultivars, five in 1993 and four in 2000 (Table 1).Likewise, the Federal University of Pernambuco (UFRPE) released two cultivars in 1996, and two other in 2005.On the other hand, the Federal University of Viçosa (UFV) released one cultivar in 1998, UFPR released four in 2001, and the Federal Rural University of Rio de Janeiro (UFRRJ) released one cultivar in 1999, and another in 2002.All these cultivars were created during PLANALSUCAR, and the experiments to evaluate the clones were initiated in 1990, before the transition to RIDESA.From the hybridizations made after 1990, 24 new cultivars were produced by RIDESA (Table 2).Since the transition of PLANALSUCAR to universities, it was established a network research model, which has been consolidated in RIDESA in recent years.The following assumptions for this network research are defined in technical cooperation agreement signed among the universities: 1) funding should be primarily private, a fact that has ensured continuous flow of financial resources in the long term for the development of the program; 2) Each university should develop its own SCBP (Sugar Cane Breeding Program) in partnership with mills and distilleries of the state in which it is located, raising private resources to do so; 3) all universities should assist in maintaining the germplasm bank and crossing experimental stations, being this cost of two million dollars annually distributed among the universities, and in proportion to the contributions received from the partnerships with mills and distilleries; 4) the university must register and protect their cultivars in the Ministry of Agriculture, and these cultivars should be licensed to other universities that are members of RIDESA; 5) the partnership model with mills and distilleries should involve introduction, evaluation and selection of clones through experiments in companies.At the same time, universities should provide, by contract, a nonexclusive license to use cultivars RB, being decided that 5% of the partnership are royalties; 6) exchange between promising clones should be performed annually among universities, so that those clones developed in other states are evaluated in experiments on the lands of mills and distilleries of the other States.
Thus, the research of RIDESA are developed and shared among all partner universities, stimulating the exchange of information, knowledge and the scientific and technology results.This greatly increases the ability and the national scope of the results of research and innovation.RIDESA has no legal personality, because it was created through a technical cooperation agreement between the universities.The top management has been made by the universities deans, who constitute a board of director, and among them a president is chosen.Among professors and researchers involved in the activities of SCBP, it is formed a council, being elected a general coordinator for a two year term.

RIDESA'S GERMPLASM BANK AND CROSSINGS
RIDESA started its research with the germplasm bank of the Flowering and Crossing Station of Serra do Ouro, in Murici, Alagoas (lat 09º 13' S, long 35º 50' W and alt 515 m asl), with average annual rainfall of 2363 mm, average minimum temperature of 18.2 ºC, and average maximum temperature of 27.9 ºC.The station is located 34 km from the coast and has a total area of 32 hectares.For its privileged location and climate, it allows the natural and profuse flowering of sugar cane that are necessary for performing hybridizations previously planned by researchers of RIDESA (Barbosa et al. 2002).This research base had its origin in 1967, when the Union of Sugar Industry in the State of Alagoas implanted the first collection of sugar cane genotypes, performing the first hybridizations selections for RB varieties.From 1971 until 1989, these activities were carried out by the IAA-PLA-NALSUCAR, which obtained and released 19 RB cultivars to the national productive sector (Barbosa et al. 2002).After 1990, Serra do Ouro was incorporeted to RIDESA, which was controlled by the Center of Agricultural Sciences of UFAL.Therefore, 78 cultivars were generated from seeds produced at Serra do Ouro, 19 of them before RIDESA, and 59 cultivars released after 1990.Currently, the germplasm bank of Serra do Ouro is composed of 2772 genotypes from national and international programs (Table 3).
RIDESA has also counted on the Flowering and Crossing Station of Devaneio, in Amaraji (lat 08° 19.8' S, long 35° 24.8' W and alt 514 m asl), Pernambuco, since 2007.It was established with the objective of complementing Serra do Ouro Station in seed production for the breeding network.There are 15 hectares of total area and privileged climate, with average annual rainfall of 2.600 mm, average minimum temperature of 18.92 ºC, and an average maximum temperature of 28.15 ºC.These conditions have allowed the required natural and profuse flowering of sugar cane for the realization of planned hybridizations.Devaneio is managed by EECAC (Sugar Cane Experimental Station of Carpina) of UFRPE.Nowadays, the germplasm bank of Devaneio consists of 814 genotypes, from national and international programs, involving basically clones and RB cultivars.
For the annual exchange of clones to be introduced at crossing stations from universities that are members of RIDESA, as well as imported genotypes, it is required quarantine.Thus, the genotypes introduced to crossing stations have been previously evaluated in quarantine station coordinated by UFAL.This station is located in Bebedouro (lat 9° 39' 59" S, long 35° 44' 6" W and alt 4 m asl), Maceió, Alagoas, and has an area of 4 hectares of forest and 1 hectare of sugar cane cultivation.In the station, there are three rooms with capacity to evaluate approximately 250 genotypes, a room for phytosanitary treatment, compartments for soil sterilization and incineration of vegetative debris and packaging, and a greenhouse of 250 m².

THE IMPORTANCE OF INTERACTION BETWEEN PUBLIC AND PRIVATE COMPANIES IN THE PRODUCTION OF CULTIVARS
The public-private partnership has existed since PLA-NALSUCAR, but has been maintained and improved by RIDESA.During PLANALSUCAR, the federal government supported most of the research.The technologies developed, including the cultivars, were validated in business.This interaction of PLANALSUCAR researchers with companies provided continuity to this action in RIDESA.However, the cost of this research began to be supported by the mills and distilleries, having in return, from the universities, the dedication of professors, technicians and students in develop- This represents 95% of Brazilian sugar cane, ethanol and bioletrectricity companies.Thus, validation and adoption of a new cultivar is greatly facilitated.That is, when a new cultivar is released, there are already companies, which are already growing them in large areas.This happens because all the companies receive the clones for evaluation experiments, some years before its possible release as a cultivar.
RIDESA's partnership model allows the definition of management of cultivars.There is hundreds of experiments conducted on the lands of mills and distilleries for this purpose.In addition, clones that present high yield in the experiments are evaluated in areas of 10 to 100 hectares in different management conditions.Thus, there is a very positive interaction between university and mills staff, increasing the number of observations and the planning of seedling nurseries, especially of new genotypes that may be released as new cultivars.

CULTIVARS PRODUCED BY RIDESA
'RB72454' is a cultivar of high yield potential when compared to all cultivars that were developed by PLANALSUCAR.A growing area with this cultivar was expanded after 1990.It occupied 20% of the total area cultivated with sugar cane in Brazil in 1995, but it dropped to 4.2% in 2012 (Figure 1), due to yield loss that came from mechanization, and susceptibility to orange rust (Puccinia kuehnii).The first occurrence of the disease in Brazil was registered in December 2009.
Another cultivar that deserves attention is the 'RB835486', which was grown mainly in central Brazil, comprising the western state of São Paulo, Minas Gerais, Goiás and Mato Grosso.It occupied about 20% of the area cultivated with sugar cane in some Brazilian cerrado mills.Considering the total area cultivated with sugar cane, it occupied about 8% in 2005 (Figure 1).This is an early maturing cultivar adapted to mechanical harvesting and has intermediate tolerance to brown rust (Puccinia melanocephala H. & P.Syd.).
Among the 35 cultivars released by RIDESA (Table 1), 'RB855536' is highlighted, presenting 5.5% of the cultivated area in 2005, and 'RB867515', with 4.3% (Figure 1).The first was cultivated mainly in the center-south, in soils with high fertility and greater moisture retention.The second, 'RB867515', which is hard and more productive in dystrophic and sandy soils, reached 22.1% of the area cultivated with sugarcane in 2011.In that same year, cultivars RB855453, RB855156 and RB835054 were adopted, being early maturing for harvesting in south-central Brazil.Table 2 presents 24 cultivars released by RIDESA, whose hybridizations were performed after 1990, i.e., such clones were created by the universities.Among them, the first to be widely adopted was the 'RB92579', occupying 6.3% of the cultivated area in 2011, and about 28% and 21% in the states of Alagoas and Pernambuco, respectively.
The adoption and use of new cultivars is the best way to quantify the results of research and investment of public and private resources.RIDESA has contributed to the production of sugar cane in Brazil, accounting for 58.9% of its total cultivated area (Figure 3).

RIDESA'S NETWORK RESEARCH AND HUMAN RESOURCES TRAINING
RIDESA consists of 246 professionals dedicated to the breeding program, distributed in 10 universities.Among them, 89 are Ph.D. Professors and Researchers, 62 are Agricultural Technicians, 75 are Operational and 20 are Administrative employees.RIDESA also has 72 research bases, consisting of Crossing Stations, Experimental Stations, Research Centers, and Selection Substations, being the last one conducted in partnership with mills (Figure 2b).
In addition to the great success in the development of sugar cane cultivars in Brazil, this network stands out in the training of human resources.Thus, the universities infrastructure has provided support for training students at the undergraduate and graduate levels in the crop of sugar cane.There are hundreds of professionals who work for private companies and public institutions.In the past five years, there had been 112 trainees of agronomy courses in universities that comprise RIDESA, and 32 undergraduate research fellows.It is noteworthy the Residence in Agronomy program, with specialization in sugar cane, that is coordinated by UFRRJ, in partnership with UFPR.This program, which is supported by the Union of Mills of the State of Paraná -ALCOPAR, has trained, since 2005, 120 new graduated agronomists.
This training occurs in the mills, under the supervision of professors from the universities that coordinate the program.
There are universities in RIDESA, which have postgraduate programs.In the last five years, 35 Masters and 24 PhD were graduated, developing researches related to the improvement of sugar cane.Also, during this period, there were 7 Post-Docs dedicated on these projects.There are several scientific methodology proposals that have contributed to the continuous evolution of knowledge directed to genetic improvement of sugar cane, as reported below.

METHODS OF SELECTION
Genetic improvement is based on two actions: i) the identification of superior individuals and ii) the creation of better new genotypic combinations by crossing among these individuals.In both phases, the selection is fundamental and is performed based on the genetic evaluation of individuals and aims to: i) infer their genetic values ii) sort them based on their genetic values.Therefore, studies to assess the efficiency of augmented designs or plot sizes in the early stages of selection and sampling of families in experiments have been carried out (Leite et al. 2006, Souza et al. 2006, Leite et al. 2009).Other studies involving selection of families via REML/ BLUP (Barbosa et al. 2004, Barbosa et al. 2005, Pedrozo et al. 2011) or calculation of the parentage coefficient for use in procedures for selecting clones and families (Peternelli et al. 2009) have also been developed by RIDESA.
The implementation of recurrent selection in sugar cane is based on experimentation with families from controlled crossings.In this context, different identification methods and selection of superior individuals for cloning and for making new crosses may be employed.The comparative efficiency of selection methods involving the evaluation of full-sib families in sugar cane is discussed below, when it is presented the expressions of the gain with the selection and accuracy for nine selection methods (Table 4).For comparisons, it was considered the evaluation of n = 100 plants (five replicates of 20 plants each, being each plot consisted of two rows of 5 m length and spaced 1.40 m apart) per full-sib family (Table 5).Ĝ a 1. Mass in sugar cane plant; 2. Between families in sugar cane plant; 3. Between families in sugar cane plant and ratoon; 4. Between families in sugar cane plant and missal in ratoon; 5. Between families in sugar cane plant and BLUP in ratoon; 6. Modified BLUPIS in sugar cane plant ratoon; 7. Individual BLUP in sugar cane plant; 8. GWS in seedlings; 9. Modified BLUPIS + GWS; k* = k(k -t) = 1.271(1.271-0.25)= 1.298: reduction factor in h 2 due to selection between families in sugar cane plant r² a * = (1 -r² âae k * ) σ a .
MHP Barbosa et al.It is noticed that among the methods based only on phenotypes, the more accurate are those that involve BLUP and modified BLUPIS (methods 5, 6 and 7 of Table 5).Among the methods based on molecular markers, GWS1 (Genome Wide Selection using 2 thousand individuals genotyped for 20 thousand markers) shows accuracy similar to the mass selection, whereas GWS2 (Genome Wide Selection using 10 thousand individuals genotyped for 30 hundred marks) shows accuracy similar to individual BLUP selection, both with the advantage of early selection in seedling level.The simultaneous method individual BLUP + GWS1 leads to accuracy practically equal to 1, but it demands the genotyping of 2000 individuals to 20,000 marks in the population of estimation of the markers effects.Furthermore, it was noticed that methods based on molecular markers have genetic gain per unit time much superior because it only takes half a year (T = 0.5) to complete the selection cycle, whereas other methods take two years (Table 5).Thus, GWS has great potential, but it demands great effort in terms of number of individuals for genotyping and number of markers.Among the methods based only on phenotypes, BLUP and modified BLUPIS lead to greater gains.These two methods have been used in RIDESA's breeding program since the last decade (Barbosa et al. 2004, Barbosa et al. 2005).The results also show that the selection among families in sugar cane plant and individual selection in ratoon cane, in the mix of families previously selected in the stage of sugar cane plant through BLUP is an appropriate strategy (Table 5).The high repeatability and genetic correlation between sugar cane plant and ratoon confirms the feasibility of this approach (Pedrozo et al. 2011).
The improvement of selection methods in sugar cane breeding has been emphasized by RIDESA.Moreover, the adoption of BLUP, other important developments were made to enhance the efficiency of the process.The method BLUPIS, for example, was developed by Barbosa and Resende (2006) and consists in determining dynamically the number of individuals to be selected in each family, with no individual assessment.The expression that determines the n k number of individuals to be selected in each k family is given by: n k = (ĝ k / ĝ j )n j , where: ĝ j refers to the genotypic effect of the best family; ĝ k refers to the genotypic effect of the k-th family, and n j refers to the number of selected individuals in the best family, which should be defined by the researcher (typically 50 to 100).The procedure was applied by Oliveira et al. (2011).
An improvement on BLUPIS is considering the family estimated genotypic value (VG) instead of its g genotypic effect, and n k-1 instead of n j .Thus, it is given n k = (VG k /VG j=1 ) n k-1 , where VG = general mean + ĝ and n k-1 refers to the number of selected individuals in the immediately preceding family in the rank.In relation to n j regarding to the ones selected in the best family, this should be the total number of individuals in the family.This new predictor, modified BLUPIS, reproduces almost exactly individual BLUP, leading to 98% correlation between the two methods, same total number of selected individuals, same number of selected individuals per family (error of only 2-4 individuals per family), and selection of the same individuals by both methods.These results are consistent with those presented in Tables 2 and 3, which show that modified BLUPIS and individual BLUP are virtually identical.These are far superior to traditional sequential selection between and within families, especially for traits with low heritability (Table 5).
It was found that the individuals selected by individual BLUP and by modified BLUPIS come from 30% best families.Thus, the methods 5 and 6 shown in Table 5 tend to be identical.Modified BLUPIS can be used according to the following steps: a) Identifying superior families in sugar cane plant, harvesting the whole plot; b) Using modified BLUPIS to determine the number of selected individuals per family; c) Measuring in the ratoon sugar cane, at individual level, only families with one or more individuals indicated by modified BLUPIS (about 30% of families) and evaluating the other families by harvesting the whole plot and doing an average per plant in plot; d) Making the individual BLUP prediction in selected families and ordering the individuals in the mix of these families.The genotype value of each individual can be calculated by g i = g fam + Res*h² ad , where: Res = y ratoon -(Xb + Zg fam + Wplot), from the harvests combined analysis, h² d = Var(g fam ) / Var(within family in ratoon); y ratoon: individual measurements performed in ratoon sugar cane and Xb+Zgfam+Wplot: individual phenotypes predicted via model fit in the joint analysis of sugar cane plant and ratoon, where b, gfam and plot are block, family genotypic and plot effects estimated and, X, Z and W are incidence matrices of the referred effects.
According to Bayesian Principle, it should not be selected more than 50% of the evaluated individuals in each family (Resende 2002).The other (indicated by BLUPIS or BLUP) must be taken randomly among the non-evaluated individuals of each family.For example, when it is desired to select 30 individuals, but from a test of only 40 individuals taken randomly from the progeny.In this case, the most efficient procedure is to select the best 20 individuals (which will probably be above progeny mean) identified based on the test and take the other 10 (not tested) randomly from the progeny.It is expected that among these 10, about 5 are above progeny mean.Thus, among 30 individuals selected, it would be collected, on average, 25 and not only 20 individuals above progeny mean.
In the last phase of clones recommendation, an efficient method of selection was developed, considering experimentation involving multiple locations and multiple harvests, while contemplating the heritability and repeatability of the traits and the genetic correlations across seasons and locations (associated with double and triple interactions).This approach includes, at the same time, productivity, stability and adaptability, and led to the method of harmonic average of relative performance of genetic values (MHPRVG), which is computed in the context of linear mixed models through REML/BLUP (Resende 2002).This method has been used frequently in the Sugar Cane Breeding Program of RIDESA (Oliveira et al. 2005, Bastos et al. 2007).
The improvement of the experimental quality has also been emphasized by RIDESA.And the experimental quality assessment should be based on selective accuracy or on the magnitude of Snedecor's F statistic, rather than the experimental coefficient of variation (Resende and Duarte 2007).Experiments with few repetitions and low genetic variability are inadequate even if associated with low coefficient of experimental variation.Optimization of experimentation is discussed in detail by Peternelli et al. (2011).

GENOME WIDE SELECTION
Genome-wide selection (GWS) or genomic selection (GS) was proposed by Meuwissen et al. (2001) as a way to increase efficiency and to accelerate the genetic improvement.GWS emphasizes the simultaneous prediction (without the use of significance tests for individual markers) of the genetic effects of thousands of genetic DNA markers (SNP, DArT, Microsatellites and others) dispersed throughout the genome of an organism, in order to capture the effects of all loci (both small and large effects) and explain all the genetic variation of a quantitative character.The prerequisite for this is that there is linkage disequilibrium at the population level, between markers alleles and genes alleles that control the character.The prediction of genetic effects is performed based on genotypic and phenotypic data of individuals belonging to a sample of population under selection.
MHP Barbosa et al.The genetic effects of markers on phenotypes of quantitative traits are summed and used to predict genetic values of only genotyped individuals, which are candidate for selection in breeding programs.The prediction and selection can be performed in very young stages of plants and animals, hence accelerating the process of breeding.Additionally, the prediction tends to be more accurate by considering the actual genetic parentage of individuals in evaluation, rather than the mathematically expected parentage mean (Resende 2007).The GWS provides a direct early selection (DES), therefore it acts early on genes expressed in adult stage.On the other hand, the traditional early selection is indirect, because it acts (through phenotypic evaluation) on genes activated in early age, hoping that they inform partly on genes expressed in adult stage.Thus, the DES provided by the GWS is especially important for the improvement of perennial and semi-perennial organisms such as sugar cane.
In summary, the superiority of GWS on selection based on phenotypes can be attributed to four factors: i) use of the real and specific parentage matrix of each character, which increases the selection accuracy ii) feasibility of DES, which increases the genetic gain per unit time, iii) permission of the repeated evaluation of each allele (providing experimental replication) without the use of clonal and progeny testing, which increases the selection accuracy iv) use of more information by combining three information types (phenotypic, genotypic and pedigree) to correct the data and do the genetic analysis, which increases accuracy.
GWS is the new paradigm in quantitative genetics in plant and animal breeding, and practical results already exist for eucalyptus (Resende et al. 2012) and pine (Resende Jr. et al. 2012).It is believed that GWS will provide a positive impact on selection methods and strategies for breeding.However, it is necessary to get practical experience with GWS in order to infer on its effectiveness.For sugar cane, GWS presents great potential, but it demands great effort in terms of number of individuals for genotyping and number of markers, as seen in Table 5. where:

N L e
The amount n Q can be taken as the effective number of segregating chromosome segments in the population, given by n Q = 2N e L g /[Ln(2N e )], where Ln is the natural logarithm and L g is the length of the genome (20 Morgans for sugar cane).Table 6 presents GWS accuracies for various scenarios in sugar cane.
In sugarcane, with a genome of 20 Morgans and an effective population size of 100, there are 755 independent chromosome segments segregating in the population, which need to be captured by the markers.If individuals are genotyped for a thousand of markers, not all segments are captured, and the coefficient of determination of genes by markers is only 11%.If the individuals are genotyped for 30,000 markers, the coefficient of determination increases to 79% (Table 6).Ten thousand genotyped individuals are required for 30,000 markers in order to obtain accuracy above 60%.But with 2000 genotyped individuals for 20,000 markers, spectacular gains per unit time are obtained, surpassing the gains from selection based on phenotypes (Table 5).With the accumulation of genotypic data over the years, the genetic evaluation system in sugar cane will involve both phenotypic and genotypic data, and RIDESA is getting ready for that.

Figure 1 .
Figure 1.Major cultivars, producing states, the total area cultivated with sugar cane in Brazil and the percentage of mechanically harvested area in1975, 1985, 1995, 2005 and  2011; and the major events that impacted sugar cane industry after Proálcool started.In parentheses are shown the percentages of the main cultivars and the main producing states in1975, 1985, 1995, 2005 and 2011.

Figure 2 .
Figure 2. (a) Areas that comprised PLANALSUCAR, which was formed by 30 experimental stations for research and their respective regional coordinators.(b) Later, PLANALSUCAR was incorporated in 1990 to federal universities (UFRPE, UFAL, UFPI, UFS, UFG, UFV, UFRRJ, UFMT, UFSCar and UFPR) and the coordination and areas were passed to RIDESA, which currently has 72 research bases, distributed by university.

Table 1 .
Thirty-five cultivars released by RIDESA from the merger of the technical and patrimonial collection of PLANALSUCAR in 1990

Table 2 .
Twenty-four cultivars released by RIDESA after hybridizations performed, since 1990

Table 3 .
Number of accessions in the germplasm bank of RIDESA Serra do Ouro, Murici, AL, Brazil a Hybrids: Number of interspecific hybrids (clones) of different originsMHP Barbosa et al.ing the breeding program of sugar cane.There are now 350 companies that have partnership agreements with universities.

Table 4 .
Expressions of genetic gains derived for comparative efficiency of selection methods in sugar cane, considering different characters with individual heritability in the narrow sense (h² a )

Table 5 .
Selective accuracies and genetic gains (in additive genetic standard deviation units -σ a ) per unit time (year), associated with the selection methods in sugar cane