MAXIMUM CARBOXYLATION VELOCITY OF RUBISCO AND MAXIMUM RATE OF ELECTRON TRANSPORT IN SAPLINGS IN RESPONSE TO VARIATIONS IN ENVIRONMENTAL FACTORS IN CENTRAL AMAZONIA

ABSTRACT

Light availability is one of the main factors that limits photosynthetic rates in saplings in the forest understory. The aims of this study were to determine how photosynthetic parameters (maximum carboxylation velocity of Rubisco - V_c-max and maximum rate of electron transport - J_max) respond to irradiance, temperature and diurnal variations in the physical environment. Gas exchange data were collected between 08h00 and 16h00, and from September to November (2010) under ambient conditions, temperature of 27± 2⁰C and relative humidity of 70 ± 3%. CO₂ saturated photosynthesis (A_pot) was measured under light saturation and [CO₂] of 2000 μmol mol^-1. Data were subjected to analysis of variance and then the Tukey test was used for mean separations. V_c-max and J_max correlated with photosynthetic rates at [CO₂] of 380 μmol mol^-1 (A₃₈₀) and A_pot. The increase in intercellular CO₂ concentration (C_i) led to increase in photosynthetic rates, up to the point of photosynthesis limitation by the electron transport rate. Below the collimation point (300-569 μmol mol^-1) photosynthetic rates were limited by V_c-max. Increases in irradiance and temperature in the forest understory led to increasing the values of A₃₈₀, A_pot, V_c-max and J_max. However, the effect of time of day on V_c-max and J_max was not significant (p > 0.05). Absence of an effect of time of day on V_c-max and J_max is important for the leaf to efficiently use the sun flecks that occasionally puncture the forest floor.

Keywords:
nonstomatal limitations; collimation of photosynthesis; diurnal variation