Aiming to evaluate the over saturated salt solution (1.5g commercially available salt in 1.5ml of tridestilled water) as tissue conservator of the canine frenic center and to compare its conservator potential with the well-established glycerin 98% conservator solution, peaces of this tissue were implanted to repair produced muscle lesions in 28 Wistar rats. Previously both solutions had been tested for bacterial and fungal contaminations, both exams with negative results. In each experimental animal, lesions were made in both Rectus abdominis muscles and repaired with the tissue preserved in both solutions. The animals were divided in seven groups and sacrificed in three, five, seven, 10, 15, 30 and 60 days after reparation for histological evaluation of the implanted region. In macroscopic exam the complete substitution of the implants by live tissue was observed, without rejection of the implanted tissue. In the microscopic evaluation we observed that the substitutions were made by fibrous conjunctive tissue deposition. At the end of the evaluation period, the implanted membranes were not present in repaired regions. In both conserved tissues there were not differences in tissue reactions. The over saturated salt, and 98% glycerin solutions presented similar results in the canine frenic center preservation indicating that both solutions may be utilized to preserve this membrane.
tissue preservation; storage solutions; alograft
