Total phenolic and flavonoid contents, antioxidant, and α-glucosidase inhibitory activities of several big chili (Capsicum annuum L.) genotypes.

ABSTRACT: Big chili has the largest fruit size compared to other types of chili. In addition to having a spicy taste, big chilies contain other beneficial biochemicals such as phenolics, flavonoids, antioxidants, and α-glucosidase inhibitors that have the potential to be developed for functional tropical plants. Information on the content is still not widely found. Therefore, this study identified biochemical compounds (TPC, TFC, DPPH, FRAP, AGI) in several genotypes of big chili peppers. From October to November 2021, we conducted a study using the microplate reader method. We repeated the experiment three times. The research design used was a completely randomized design with a single factor genotype. A total of 22 genotypes of big chili were used in this study. The big chili genotype F6074136-2-3-2-3 had the highest content of α-glucosidase inhibitor and antioxidant FRAP compared to other genotypes 67.62% and 71.20 mol TE/g extract. The genotypes CK 12 and F6074077-1-1-3-1 indicated the highest phenolic and flavonoid contents. The correlation between biochemical parameters showed that TPC-AGI and TPC-DPPH were significantly negatively correlated. The conclusion is that the information in this study can be the basis for the development of functional tropical plants in future research.


INTRODUCTION
Plants have complex biochemical compounds resulting from the process of photosynthesis.These biochemical compounds can be utilized for the development of the herbal pharmaceutical industry.Biochemical compounds commonly reported in plants are antioxidants (WEN et al., 2020).Antioxidants are compounds that inhibit oxidation reactions.The way antioxidants work varies, one of which is the binding of free radicals formed in the human body (SUEISHI & NII, 2019).The formation of free radical compounds causes a chain reaction that can damage cells or tissues, causing degenerative diseases, cancer, and diabetic (JEONG et al., 2021).
Ciência Rural, v.53, n.7, 2023.Sahid et al.Humans naturally produce antioxidant compounds in the body (lAMMI et al., 2019).The human body requires sufficient antioxidants to help prevent and overcome the free radical compounds contained in it.However, due to the influence of various factors that cause a lot of free radical compounds to form in the body, it is necessary to take additional antioxidants from outside the human body.
Generally, antioxidants in plants are reported in horticultural groups (BURRI et al., 2017;HEGEDŰS et al., 2019).Plants produce excess antioxidants when experiencing growth disorders as a self-protection mechanism (GIORDANO et al., 2021).One of the horticultural crops consumed by humans is chili.Chili is a plant that generally gives a spicy taste to food consumed by humans (SAHID et al., 2020).Behind its spiciness, chili has many secondary metabolites compounds that can potentially be developed including phenolic compounds (MORENO et al., 2018;ANJOS et al., 2022), flavonoids (VAZQUEZ-FlORES et al., 2020), antioxidants (AlAM et al., 2018), and even α-glucosidase inhibitory activities (NANOK & SANSENyA, 2019).α-glucosidase inhibitors are enzymes that function to inhibit the absorption of sugar in the blood (STEFANO et al., 2018).Recent research has shown that α-glucosidase inhibitors may be helpful as COVID-19 antivirals (SPENCER & ETHAN, 2020).
Chili is one type of horticultural plant that has many consumers and has high economic value (HERATH et al., 2021).The majority of the world's population uses chili as the basic ingredient for spicy food.In addition, the pharmaceutical industry uses chili peppers for making chili patches (FATTORI et al., 2016;SAlEH et al., 2018).However, based on previous research, it turns out that chili has the potential to be developed as an herbal medicine for diabetic because it has antioxidant compounds and α-glucosidase inhibitors.Information on inhibitor α-glucosidase content in chili is still rare.
The distribution of chili plants in the world is grouped by species.The most widely circulated species in Asia, especially Indonesia, is Capsicum annuum (KARIM et al., 2021).This is partly due to the climatic factors of the tropics, which are very optimum for Capsicum annuum.Based on the size of the fruit, chilies are divided into three namely cayenne pepper, large chili, and curly chili (ISTIQlAl et al., 2018).Big chili has the advantage of large fruit size so that it affects the productivity produced.Therefore, it is expected that the content of antioxidants and α-glucosidase inhibitors produced is more than other types of chili.Seeing this potential, we identified the content of phenolic, flavonoid, antioxidant and inhibitor content in large chili species Capsicum annuum.

MATERIALS AND METHODS
The genetic material used in this study is shown in table 1.The genetic material consisted of 5 genotypes of pure-line chili and 17 genotypes of chili that are commonly cultivated in Indonesia.All species used comes from Capsicum annuum, which is also a type of sweet pepper that is commonly cultivated in the world.Chili planting was carried out at the Alam Sinarsari D80 Greenhouse by fertilizing using AB Mix dissolved in water.The chilies used for sample extraction in this study are perfectly ripe chilies.

Sample Extraction
Fresh chilies were dried in an oven at 80 0 C for 24 hours.The dried fruit is crushed into powder using a blender until smooth.Extraction using a ratio of 1:20 between chili powder and ethanol 80%.This study used 3 grams of chili powder dissolved in 60 mL of 80% ethanol by maceration method for 2x24 hours in a water bath shaker.The extract that had been shaken for 2x24 hours was then filtered using filter paper and transferred to a glass bottle.Then, repeated maceration and re-filtering was carried out until the final volume of the extract was 60 mL.The filtered extract was then stored at 15 0 C for 24 hours before use.

Data analysis
This study used SAS 9.0 and R 4.0.5 applications for further testing in this article.Further tests performed included: ANOVA, 5% DMRT test, Pearson correlation analysis, and HCA analysis.HCA analysis determined the relationship between the genotypes used and the R 4.0.5 packages PheatMap library.

RESULTS AND DISCUSSION
Table 2 shows the average biochemical content of several genotypes of large chili n in table 2. The total phenolic content was measured in the range 8.11 (C 5) -17.99 (CK 12).The CK 12 genotype has a total phenolic content that is significantly different from all genotypes except the CK 11 genotype.Phenolic compounds are secondary metabolites in plants that are reported in plant cell walls (MNICH et al., 2020).This compound serves to prevent the decay of plant tissue (ŽEBELJAN et al., 2021).This can indicate that C5 chilies will easily spoil compared to CK 12 chilies.
Antioxidants in the CK 12 genotype as measured using the FRAP method showed the highest yield of 71.67.CK12 was a bell pepper (paprika) that isy used as a base for pizza making around the world.Antioxidant FRAP is an antioxidant content that is measured to see the ability of the extract to convert Fe 3+ compounds into Fe 2+ (SVEČNJAK  et al., 2020).This means that the CK 12 genotype contains antioxidants that function to reduce iron.The total phenolic content of the C 5 genotype was inversely proportional to the antioxidant value of DPPH produced, which was 13.73 where this value was the highest value compared to other genotypes.This shows that this genotype contains antioxidants that are able to ward off free radicals better than other genotypes.In accordance with research (ADEGBOlA et al., 2020) that DPPH antioxidants are measured using the principle of free radical scavenging.The measurement results of α-glucosidase inhibitors (Figure 1) showed that the pure line genotype F6074136-2-3-2-3 was the highest (67.62%) compared to other genotypes but was not significantly different from the C 5 genotype (65.53%).The content of α-glucosidase measured in all genotypes was in the range of 9.55-67.62%.The pure line F6074136-2-3-2-3 has relatively high biochemical compounds.C 5 genotype was also observed to have the highest DPPH antioxidant compared to all observed genotypes.This is consistent with the results of the study (ZAyAPOR et al., 2021), which showed that the genotype with the highest antioxidant content of DPPH also showed the highest content of α-glucosidase inhibitor.To explain the relationship between the observed variables for biochemical content, a Pearson correlation analysis was performed (Figure 2).

CONCLUSION
equal letters (vertical) do not differ statistically from each other by the duncan multiple range test at 5%.Total phenolic and flavonoid contents, antioxidant, and α-glucosidase inhibitory... Ciência Rural, v.53, n.7, 2023.5 Twelve genotypes contain α-glucosidase inhibitors above 50%.The content of α-glucosidase inhibitor has a positive correlation with antioxidant DPPH, but has a significant negative correlation with TPC and TFC.The results of the HCA analysis in this article are able to provide information on the relationship between the genotype and the observed biochemical content.The genotypes were divided into 5 major groups.Functional chili plants development are directed

Figure 1 -
Figure 1 -Inhibitor α-glucosidase activity (AGI) in big chili genotypes.Means followed by equal letters (vertical) do not differ statistically from each other by the Duncan multiple range test at 5%.
Sahid et al.    to alternative plants for diabetic treatment.In addition, plant breeders can use the information in this article as a basis for selection of elders in both conventional and biotechnology plant breeding activities.

Table 1 -
list of genotypes used in this study.