The low in vitro regeneration of some indica rice cultivars, limits the use of anther culture techniques as a tool to reduce the time for releasing new cultivars. The identification of genomic regions associated with callus formation and in vitro regeneration from anther culture, will facilitate selection of these traits through marker assisted techniques. Two backcross populations were used to study and map these traits, Taipei 309// BRS 7 "Taim"/Taipei 309 (53 genotypes) and BRS 7 "Taim"// BRS 7 "Taim"/Taipei 309 (74 genotypes). Two hundred immature anthers, from each genotype used in this study, were inoculated in liquid NL medium for callus induction and, after 40 days, the formed callus were transferred to solid MS medium for plant regeneration. Each anther donor plant was used for DNA extraction and seven RAPD primers were used to assess genomic regions associated to callus formation and plant regeneration. As results, callus formation ranged from 2.27 up to 3.36% and plant regeneration ranged from 1.38 up to 1.82%, no significant differences were observed. Six linkage groups were obtained, being three from each population. One QTL was detected associated to callus formation, with LOD score 3.10, in the population Taipei 309 // BRS 7 "Taim" / Taipei 309. No QTL association was detected to the trait plant regeneration.
indica subspecies; microspores; androgenese; callus; linkage map
