In vitro tests were performed to evaluate the effect of cryopreservation with or without addition of cryoprotectants (DMSO or glycerol) in the predatory activity on Cooperia sp and Haemonchus sp infective larvae, radial growth and conidial production of two isolates of nematode-trapping fungus (Monacrosporium sinense and M. appendiculatum). The predatory activity on Cooperia and Haemonchus sp infective larvae of the fungus submitted to the different preservations methods did not affected. The radial growth of the fungus cryopreserved with glycerol was higher (p<0.05), when compared to others treatments. The conidial production was higher (p<0.05) in the M. appendiculatum isolate preserved at 4ºC in refrigerator.
cryopreservation; nematophagous fungi; Monacrosporium spp; Haemonchus sp; Cooperia sp
