The purpose of this work was to evaluate two protocols of disinfestations in the micropropagation on young plants of three banana cultivars: Prata-Anã, FHIA 18 and SH3640. Protocols 1 and 2 of disinfestations (Dis. 1 and Dis. 2) were carried out using the products: fungicidal solution of Derosol, commercial alcohol, solution of sodium hypo chlorite and calcium hypo chlorite and tween 20, presenting variations in their concentration in every one of the protocols. The evaluations for contamination rate were taken daily by means of the total number of contaminated and non-contaminated tubes. A random block design in a factorial scheme with four repetitions was used. Exclusively bacteria had caused all contamination. Independently of the used treatment the contamination levels had been up to 29% for all the three cultivars tested. These results indicate the necessity of adjusting the new methodologies of asepses of banana explants.
Musa spp.; micropropagation; contamination
