A 6-month-old splenectomized calf was inoculated with blood from a cattle with Tick Fever. The clinical signs, rectal temperature and packed cell volume were observed between the 0 and 40th days. Blood and blood clot samples were collected from the calf on the 8th, 10th, 22th, 24th and 26th days. The following techniques were evaluated by the Anaplasma marginale parasitemia: thin blood smear of blood clot fragment and smear of a drop of blood clot. The slides were stained by the dilute and pure Giemsa methods. The results were acceptable and the authors propose the use of the three techniques to confirm an Anaplasma diagnosis.
blood smear; clot smear; clot impression smear; hematozoan; giemsa
