A thousand two hundread and seventy-one oocytes were allocated in four treatments, in order to evaluate the influence of the addition of FSH and LH on in vitro production of bovine embryos, with oestrous cow serum (OCS) or mare´s serum obtained at the first day of estrus (OMS). In all treatments oocytes were matured with TCM199 + 5.95mg/ml Hepes and 0.025mg/ml sodium pyruvate and 2.2mg/ml of sodium bicarbonate, supplemented with 10% OMS (ES), 10% of OCS (VS), 10% of OMS + LHb + rFSHh (EH) and 10% of OCS + LHb + rFSHh (VH). All the treatments groups were matured in a controlled incubator at 39ºC with 5% CO2 and saturated humidity for 22-24h. IVF was performed in TALP-FERT for 18-20h, with a pool of Bos taurus semen selected by swim up procedure in TALP-SPERM with 1x106 spermatozoa/ml dose, and cultured for 8 days under SOF medium + 5% OMS (ES and EH) or OCS (VS and VH). The 72% of cleavage rate obtained for treatment VH and 61% obtained in the VS group were significantly less (p<0.05) than treatments EH (80%), ES (80%). Blastocyst rates at D7 after insemination for treatments ES (32%), EH (28%) and VH (27%) were significantly superior than the 20% obtained on VS group (p<0.05). Evaluations at D9 demonstrated higher blastocyst rates in treatment ES (31%) and EH (29%) when compared with treatment VS (22%), but no differences were detected when compared to the VH (24%). These results suggest that the utilization of OCS, the supplementation with FSH and LH renders an increase on blastocyst rates and, that the utilization of MS, even without FSH and LH, shows similar results as those obtained with OCS plus hormones.
embryo; LH; FSH; oestrus mare serum; oestrus cow serum
