Characterization of canine bone marrow mononuclear cells

Caracterização de células mononucleares da medula óssea canina

Bone marrow mononuclear cells (BM-MNC) have been used to treat various diseases. These cells are chemo-attracted to sites of injury and may produce cytokines and trophic factors that act in tissue regeneration. Nevertheless, a challenge associated with this procedure is the lack of studies on the characterization of canine BM-MNC. This study contributed with BM-MNC characterization data. Bone marrow mononuclear cells from four donors were labeled with anti-CD8a, CD9, CD14, CD29, CD34, CD44, CD45, and CD90 for phenotypic characterization. In this study, was obtained strong expression of CD9 (79.48% ± 7.71%), CD29 (96.10% ± 1.71%), CD44 (89.60% ± 3.14%) and CD45 (94.33% ± 4.09%). Therefore, these markers should be considered in the definition of panel for characterization of canine BM-MNC.

Key words:
cells characterization; phenotypic characterization; canine CD markers