Rust is one of the most important diseases in grapevine, with occurrence recorded in many production areas. In this research it was evaluated three methods of Phakopsora euvitis inoculation on grapevine seedlings cv. 'Isabel' with the objective of selecting an inoculation method for rapid pathogenicity determination. Grapevine seedlings were inoculated with P. euvitis and maintained under conditions of 25±4C and UR 85±10% for 13 days. The experimental design was completely randomized, with 10 replications. The treatments were: T1 - brush of pathogen spores; T2 - spores suspension spraying (2.7x10³ spores mL-1); T3 - disks of grapevine leaves with symptoms stuck in five leaves per plant and T4 - control. The spore brushing method was the most efficient, showing early symptoms at five days, and 100% of the leaves showing pathogen symptoms 13 days after treatment.
infection; urediniospores germination; pathogenicity test
