The aim of this study was to determine the frequency of antibodies anti-Infectious Bursal Disease Virus as well as to detect the virus in broilers and chicken backyard, raised in two different regions at Bahia's poultry production area. A total of 758 serum samples were collected from broilers and 320 from chicken backyard, in order to assess the frequency of antibodies using an indirect ELISA. For virus detection and characterization it was collected 6 bursal pools from broilers and 3 from chicken backyard, which were further analyzed with PCR/RFLP. The results showed that there is no uniform protection in commercial flocks of the two different regions, suggesting that it may be occurring vaccination errors and that it may be occurring challenge from viruses at the environment. High titers were observed in no vaccinated chicken backyard. Molecular tests revealed that 3 broilers pools were positive in which 2 matched the vaccine strain (G3) and 1 a variant strain (G15). One sample from free-ranging chickens was positive for the variant strain (G15). These results demonstrate the need for monitoring both types of exploration.
Infectious Bursal Disease; ELISA; serology; PCR; molecular biology; chickens