Agro-industrial residues utilization of palm oil for lipase production by Aspergillus niger

Edmar das Mercês Penha Ludmila de Araújo Nunes Viana Leda Maria Fortes Gottschalk Selma da Costa Terzi Erika Fraga de Souza Sidinéa Cordeiro de Freitas Juliana de Oliveira Santos Thaís Fabiana Chan Salum About the authors

ABSTRACT:

In this paper it was evaluated the performance of two strains of Aspergillus niger (mutant 11T53A14 and wild type C) previously selected as promising for lipase production, from cultivation media formulated from palm kernel cake (kernel) and alkaline sludge from refining were evaluated palm oil (palm oil sludge), palm oil (palm) waste industrialization by solid state fermentation (SSF). Experiments of enzyme production in SSF were conducted in aerated columns, incubated in a water bath at 32°C with controlled inlet of 1.0vvm. The best results were obtained with the mutant strain A. niger 11T53A14 in medium containing palm kernel cake moistened with a solution of ammonium sulfate (1.2%) and with the addition of 3% of palm oil sludge. The maximum lipase activity in this medium was 72.57U gdw-1 in 48 hours and 47.5% higher than in the medium without sludge palm. A comparison of the performance of the mutant strain with the wild-type strain showed that the medium composed of palm kernel cake added with ammonium sulfate and blurs palm induced both strains to produce lipases with good activity levels and reduced the time of the fermentation process.

Key words:
lipase; agro-industrial residue; Aspergillus niger

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