Low density genomic data for animal breeding: critical analysis and perspectives of the GoldenGate Beadxpress technology

Utilização de dados genômicos de baixa densidade no melhoramento animal: análise crítica e perspectivas da Tecnologia GoldenGate BeadXpress

ABSTRACT:

The increasing development of DNA sequencing and genotyping technologies has made possible to analyze the genomes of several species. Genomic studies of production animals have greatly increased the understanding of mechanisms that control the interactions of genetic and environmental factors involved in the expression of traits of economic importance. Several technologies have been presented by different companies for the genotyping of low-density SNP panels, which may be used in different applications with different goals, such as paternity testing, diagnosis of genetic diseases, and identification of genetically superior animals based on polymorphisms characterized in candidate genes. The present review critically analyzes the GoldenGate Beadxpress technology and puts its use in these applications into perspective.

Key words:
SNP genotyping; GoldenGate technology; haplotypic blocks; custom SNP panels; association studies