ABSTRACT:
This study investigated the occurrence of antibodies for BVDV-1 in swine herds located in the region of Mossoró city of the state of Rio Grande do Norte, Brazil. A sample size of 412 animals was estimated assuming unknown prevalence (set at 50%). Virus neutralization assay was used to the detect the presence of antibodies for BVDV-1 and the results found were analysed using multivariable logistic regression model. The obtained prevalence was 4% at animal level and 45% at the animal and herd level. The titers were highly variable between animals and within farms. The multivariable logistic regression analysis showed an association between being housed outside and exposure to BVDV-1 (OR=0.24, 95% CI:0.06, 0.96, P=0.04). Highly correlated data and low prevalence of antibodies at the animal level resulted in insufficient power to detect significant differences with other selected risk factors. In conclusion, the prevalence is within the range reported for other countries.
Key words:
swine;
pestiviruses
; virusneutralization; BVDV; CSFV
RESUMO:
Este estudo investigou a ocorrência de anticorpos contra o BVDV-1 em rebanhos suínos localizados na região da cidade de Mossoró do Estado do Rio Grande do Norte, Brasil. Um tamanho de amostra de 412 animais foi estimado assumindo uma prevalência desconhecida (fixada em 50%). O teste de virusneutralização foi utilizado para detectar a presença de anticorpos ao BVDV-1 e os resultados obtidos foram analisados usando o modelo de regressão logística multivariada. A prevalência obtida foi de 4% em nível animal e de 45% dos rebanhos. Os títulos foram muito variáveis entre os animais e dentro de fazendas. A análise de regressão logística multivariada apontou associação entre animais criados soltos e a exposição ao BVDV-1 (OR=0,24; 95% IC: 0,06; 0,96; P=0.04). A alta correlação entre os dados junto com a baixa prevalência de anticorpos a nível animal pode ter sido insuficiente para que as diferenças reais fossem detectadas. Em conclusão, a prevalência está dentro do intervalo referido em outros países.
Palavras-chave:
suínos;
pestivírus
; virusneutralização; BVDV; CSFV
INTRODUCTION:
The Pestivirus genus includes Classic Swine Fever Virus (CSFV), Bovine Viral Diarrhea Virus (BVDV-1 and BVDV-2), Border Disease Virus (BDV) (BECHER et al., 2003BECHER, P. et al. Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. Virology, v.311, n.1, p.96-104, 2003. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/12832207
>. Accessed: Jun. 17, 2015. doi: 10.1016/S0042-6822(03)00192-2.
http://www.ncbi.nlm.nih.gov/pubmed/12832...
), and other putative species such as Hobi-like virus (SCHIRRMEIER et al., 2004) and Bungowannah (KIRKLAND et al., 2007KIRKLAND, P.D. et al. Identification of a novel virus in pigs - Bungowannah virus: a possible new species of pestivirus. Vírus Research, v.129, n.1, p.26-34, 2007. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/17561301
>. Accessed: Oct. 26, 2015.
http://www.ncbi.nlm.nih.gov/pubmed/17561...
). These virus are enveloped single-stranded RNA viruses that cause important diseases in several food animals species (KIRKLAND et al., 2012KIRKLAND, P.D. et al. Pestiviruses. In: ZIMMERMAN, J.J. et al. (Eds.). Diseases of swine. 10.ed. UK: John Wiley & Sons, 2012. p.538-553. ISBN: 978-0-8138-2267-9.). In 1973, a field strain of BVDV was isolated for the first time from a naturally infected sow and her piglet, evidencing that swine could be a host for ruminant Pestivirus (FERNELIUS et al., 1973FERNELIUS, A.L. et al. Bovine viral diarrhoea vírus in swine: characteristics of vírus recovered from naturally and experimentally infected swine. Canadian Journal of Medicine, v.37, n.1, p.13-20, 1973. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1319716/
>. Accessed: Jun. 17, 2015. PMC1319716.
http://www.ncbi.nlm.nih.gov/pmc/articles...
). Although BVDV infection does not commonly produce clinical signs in swine, a "mild Classical Swine Fever" syndrome has been reported (LIESS & MOENNIG, 1990LIESS, B.; MOENNIG, V. Ruminant pestivirus infection in pigs. Revue Scientifique et Technique, v.9, n.1, p.151-161, 1990. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/1966720
>. Accessed: Jun. 17, 2015.
http://www.ncbi.nlm.nih.gov/pubmed/19667...
; TAO et al., 2013TAO, J. et al. Bovine viral diarrhea virus (BVDV) infections in pigs. Veterinary Microbiology, v.165, n.3-4, p.185-189, 2013. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/23587625
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2013.03.010.
http://www.ncbi.nlm.nih.gov/pubmed/23587...
), as well as reproductive problems in breeding animals such as abortions, weak born piglets, and digestive problems (KIRKLAND et al., 2012KIRKLAND, P.D. et al. Pestiviruses. In: ZIMMERMAN, J.J. et al. (Eds.). Diseases of swine. 10.ed. UK: John Wiley & Sons, 2012. p.538-553. ISBN: 978-0-8138-2267-9.).
The prevalence of BVDV infections in swine varies according to region, ranging anywhere from 0 to 43.5% (LOEFFEN et al., 2009LOEFFEN, W.L.A. et al. Seroprevalence and risk factors for the presence of ruminant pestiviruses in the Dutch swine population. Veterinary Microbiology, v.136, n.3-4, p.240-245, 2009. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/19128896
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2008.11.011.
http://www.ncbi.nlm.nih.gov/pubmed/19128...
; O'SULLIVAN et al., 2011O'SULLIVAN, T. et al. Seroprevalence of bovine vira diarrhea vírus neutralizing antibodies in finisher hogs in Ontario swine herds and targeted diagnostic testing of 2 suspect herds. Canadian Veterinary Journal, v.52, n.12, p.1342-1344, 2011. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215470/
>. Accessed: Jun. 17, 2015. PMCID: PMC321.
http://www.ncbi.nlm.nih.gov/pmc/articles...
; DENG et al., 2012DENG, Y. et al. High prevalence of bovine viral diarrhea virus 1 in Chinese swine herds. Veterinary Microbiology, v.159, n.3-4, p.490-493, 2012. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/22613254
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2012.04.023.
http://www.ncbi.nlm.nih.gov/pubmed/22613...
). In Ireland, the prevalence was reported to be very low, 0.14% (GRAHAM et al., 2001GRAHAM, D.A. et al. Pestivirus in sheep and pigs in Northern Ireland. Veterinary Record, v.148, p.69-72, 2001. Available from: <Available from: http://veterinaryrecord.bmj.com/content/148/3/69
>. Accessed: Jun. 17, 2015. doi: 10.1136/vr.148.3.69.
http://veterinaryrecord.bmj.com/content/...
), whereas in The Netherlands, 2.5% of sows and 0.42% of finishing pigs were seropositive (LOEFFEN et al., 2009LOEFFEN, W.L.A. et al. Seroprevalence and risk factors for the presence of ruminant pestiviruses in the Dutch swine population. Veterinary Microbiology, v.136, n.3-4, p.240-245, 2009. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/19128896
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2008.11.011.
http://www.ncbi.nlm.nih.gov/pubmed/19128...
). Furthermore, in China, between 20% to 34% of swine from 11 different provinces during 2007-2010 were found to have been exposed to BVDV-1 (DENG et al., 2012DENG, Y. et al. High prevalence of bovine viral diarrhea virus 1 in Chinese swine herds. Veterinary Microbiology, v.159, n.3-4, p.490-493, 2012. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/22613254
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2012.04.023.
http://www.ncbi.nlm.nih.gov/pubmed/22613...
).
Epidemiological studies show that cattle are the main hosts for BVDV and an infection source for swine and other wild ruminants, either through direct contact (LIESS & MOENNIG, 1990LIESS, B.; MOENNIG, V. Ruminant pestivirus infection in pigs. Revue Scientifique et Technique, v.9, n.1, p.151-161, 1990. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/1966720
>. Accessed: Jun. 17, 2015.
http://www.ncbi.nlm.nih.gov/pubmed/19667...
) or through the use of contaminated bovine milk and milk derivatives used as components in swine feed (TERPSTRA & WENSVOORT, 1988TERPSTRA, C.; WENSVOORT, G. Natural infections of pigs with bovine viral diarrhoea virus associated with signs resembling swine fever. Research in Veterinary Science, v.45, n.2, p.137-142, 1988. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/2848298
>. Accessed: Jun. 17, 2015. PMID: 2848298.
http://www.ncbi.nlm.nih.gov/pubmed/28482...
).
There is a lack of peer-reviewed publications on BVDV infection in swine in Brazil; therefore, the primary objective of the present study was to estimate the prevalence of BVDV-1 antibodies in swine herds located in the Rio Grande do Norte state of Brazil, and determine correlation between seropositivity and selected risk factors.
MATERIALS AND METHODS:
The study was conducted in the region of Mossoró municipality, in the state of Rio Grande do Norte, which has a total of 6.046 pigs, the greatest amount in the state (IBGE, 2012IBGE (BRAZILIAN INSTITUTE OF GEOGRAPHY AND STATISTICS). Produção da Pecuária Municipal. Rio de Janeiro, 2012. v.40, p.1-71. Available from: <Available from: http//ftp.ibge.gov.br/Producao_Pecuaria/Producao_da_Pecuaria_Municipal/2012/ppm2012.pdf
>. Accessed: Jul. 19, 2014.
http//ftp.ibge.gov.br/Producao_Pecuaria/...
). A sample size of 385 animals was estimated assuming unknown prevalence (set at 50%), level of confidence of 95% and margin of error of 5%, 412 blood samples were collected between January and June of 2013 of animals from one slaughterhouse facility, also located in Mossoró. Eligibility criteria included that animals had to be slaughtered in the selected slaughterhouse (convenience sample) and farm owners were required to sign a consent form agreeing to participate in the study. Animal data was collected from producers at the time of sample collection.
Blood samples were centrifuged, and serum samples were stored at -20°C and the virus neutralization assays was undertaken. All samples were processed in duplicate, following the World Organization for Animal Health (OIE) guidelines (OIE, 2015OIE (WORLD ORGANISATION FOR ANIMAL HEALTH). Bovine viral diarrhoea. In: _____. Manual of diagnostics tests and vacines for terrestrial animals. 2015. p.608-710. Available from: <http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.04.08_BVD.pdf>. Accessed: Oct. 26, 2015.
http://www.oie.int/fileadmin/Home/eng/He...
), using Madin Darby Bovine Kidney (MDBK) cell culture and BVDV strain Singer-1a using 100 TCID50 concentration.
Samples were not tested for Border disease, because this disease is exotic in Brazil; therefore, it was not possible to perform this test. Regarding the Classical swine fever diagnosis, it must be performed in official laboratories and we did not have authorization from Ministry of Agriculture, Livestock. The BVDV-2 was not performed, because of the insufficient volume of samples.
Analyses were conducted at the animal level, and the outcome was binary (positive or negative) based on an antibody titer of >/=10. The final titer was obtained from the geometric mean of titers and the final data were transformed in log base format .
The 20 largest supliers were visited by the study investigators to gather information about the herd demographics for the risk factor analyses. The questionnaire used is available upon request to the authors. Descriptive analysis was conducted using SAS 9.3. Two types of statistical models were constructed using STATA-IC 10 in order to investigate animal age and farm practices as risk factors for BVDV infection. Independent variables considered during analyses included animal age (market age/"young" or culling animals/"old"), herd size (continuous variable), housing type (free range or confined), use of human food scraps to feed pigs, use of treated water, presence of bovine species in the farm, presence of sheep or goats in the farm, and the occurrence of abortion in the herd.
First, a multivariable logistic regression model was constructed, which did not account for the clustering of animals within farms ("unadjusted model"). Univariable analyses were conducted and all variables with P<0.20 were offered to the full model. Subsequently, a backwards stepwise approach was taken, with statistical significance declared when P<0.05. Confounders were defined as variables that modified the coefficients of one or more of the other predictors' coefficients by 20% or more, and were kept in the model regardless of their statistical significance. One-way interactions with variables retained in the final model were tested and kept if P<0.05.
For the second model, a multivariable model using a generalized estimating equation (GEE) approach was attempted in order to account for the clustering of animals within farms ("adjusted model"). The correlation structure used was independent. The same steps described above were used, but because none of the predictors had P<0.20 in the univariable analysis, none moved forward to a multivariable model.
RESULTS AND DISCUSSION:
Prevalence of antibodies for BVDV-1 at the animal and farm level were 4.13% (17/412, 95% CI: 2.21%-6.05%), and 45.0% (9/20, 95% CI: 23.20%-66.80%), respectively. Mean within-herd prevalence was estimated to be 4.23% (range: 2.0% to 29.0%). The titers were highly variable between animals and, in a few cases, within farms (Table 1).
The observed prevalence for BVDV-1 antibodies at the animal level is within the prevalence reported in the literature (GRAHAM et al., 2001GRAHAM, D.A. et al. Pestivirus in sheep and pigs in Northern Ireland. Veterinary Record, v.148, p.69-72, 2001. Available from: <Available from: http://veterinaryrecord.bmj.com/content/148/3/69
>. Accessed: Jun. 17, 2015. doi: 10.1136/vr.148.3.69.
http://veterinaryrecord.bmj.com/content/...
; LOEFFEN et al., 2009LOEFFEN, W.L.A. et al. Seroprevalence and risk factors for the presence of ruminant pestiviruses in the Dutch swine population. Veterinary Microbiology, v.136, n.3-4, p.240-245, 2009. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/19128896
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2008.11.011.
http://www.ncbi.nlm.nih.gov/pubmed/19128...
; DENG et al., 2012DENG, Y. et al. High prevalence of bovine viral diarrhea virus 1 in Chinese swine herds. Veterinary Microbiology, v.159, n.3-4, p.490-493, 2012. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/22613254
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2012.04.023.
http://www.ncbi.nlm.nih.gov/pubmed/22613...
). At the herd level, the observed prevalence is higher than previously reported. According to O'Sullivan (2011), there was a 0% prevalence in Ontario (Canada). KIRKLAND et al. (2012KIRKLAND, P.D. et al. Pestiviruses. In: ZIMMERMAN, J.J. et al. (Eds.). Diseases of swine. 10.ed. UK: John Wiley & Sons, 2012. p.538-553. ISBN: 978-0-8138-2267-9.) and LIESS & MOENNING (1990LIESS, B.; MOENNIG, V. Ruminant pestivirus infection in pigs. Revue Scientifique et Technique, v.9, n.1, p.151-161, 1990. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/1966720
>. Accessed: Jun. 17, 2015.
http://www.ncbi.nlm.nih.gov/pubmed/19667...
) reported a prevalence between 2% to 43% in US herds; and LOEFFEN et al. (2009) reported that 11% of herds in The Netherlands were seropositive. According to SCHROEDER et al. (2012SCHROEDER, S. et al. Evaluation of classical swine fever virus antibody detection assays with an emphasis on the differentiation of infected from vaccinated animals. Scientific and Technical Review of the Office International des Epizooties. v.31, n.3, p.997-1010, 2012. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/23520753
>. Accessed: Nov. 09, 2015. PMID: 23520753.
http://www.ncbi.nlm.nih.gov/pubmed/23520...
), three out of the seven most used ELISA commercial kit for CSF serological diagnosis were poorly able to differentiate anti-BVDV antibodies from anti-CSFV antibodies in swine serum. Thus, the presence of anti BVDV antibodies in pig herds could lead to false-positive CSF results, hindering CSF surveillance and eradication programs based on serological diagnosis (DE SMIT et al., 1999DE SMIT, A.J. et al. Laboratory decision making during the classical swine fever epidemic of 1997-1998 in the Netherlands. Preventive Veterinary Medicine, v.42, p.185-199, 1999. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/10619155
>. Accessed: Oct. 26, 2015. PMID: 10619155.
http://www.ncbi.nlm.nih.gov/pubmed/10619...
).
Members of the Pesitivirus genus have great antigenic similarity and there are reports of cross-reaction in the virus-neutralization test (SIMMONDS et al., 2011SIMMONDS, P. et al. Family Flaviviridae. In: KING, A.M.Q. et al. (Eds.). Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. San Diego, CA: Elsevier Academic, 2011. p.1003-1020.). However, scientific papers have shown that antigenic differences were still detected among genotypes and subgenotypes within this genus by the same virus-neutralizatiton assay (BECHER et al., 2003BECHER, P. et al. Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. Virology, v.311, n.1, p.96-104, 2003. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/12832207
>. Accessed: Jun. 17, 2015. doi: 10.1016/S0042-6822(03)00192-2.
http://www.ncbi.nlm.nih.gov/pubmed/12832...
; RIDPATH et al., 2010RIDPATH, J.F. et al. Prevalence and antigenic differences observed between Bovine viral diarrhea virus subgenotypes isolated from cattle in Australia and feedlots in the southwestern United States. Journal of Veterinary Diagnostic Investigation, v.22, n.2, p.184-191, 2010. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/20224075
>. Accessed: Nov. 09, 2015. doi: 10.1177/104063871002200203.
http://www.ncbi.nlm.nih.gov/pubmed/20224...
; BEHERA et al., 2011BEHERA, S.P. et al. Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from cattle in India. Comparative Immunology, Microbiology and Infectious Diseases, v.34, n.2, p.189-196, 2011. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/21112633
>. Accessed: Nov. 09, 2015. doi: 10.1016/j.cimid.2010.11.002.
http://www.ncbi.nlm.nih.gov/pubmed/21112...
; PECORA et al., 2014PECORA, A. et al. First finding of genetic and antigenic diversity in 1b-BVDV isolates from Argentina. Research in Veterinary Science, v.96, n.1, p.204-212, 2014. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/24295740
>. Accessed: Nov. 09, 2015. doi: 10.1016/j.rvsc.2013.11.004.
http://www.ncbi.nlm.nih.gov/pubmed/24295...
). In addition, among the bovine pestivirus groups, the Hobi-like virus was found to be the most antigenically different from BVDV-1 and 2 (BAUERMANN et al., 2012BAUERMANN, F.V. et al. Antigenic relationships between Bovine viral diarrhea virus 1 and 2 and HoBi virus: possible impacts on diagnosis and control. Journal of Veterinary Diagnostic Investigation, v.24, n.2, p.253-261, mar. 2012. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/22379042
>. Accessed: Nov. 09, 2015. doi: 10.1177/1040638711435144.
http://www.ncbi.nlm.nih.gov/pubmed/22379...
). Considering all pestivirus genus, Pronghorn, Bungowannah and Atypical Putative Porcine Virus (App V), are more divergent to those virus than Hobi-like (BAUERMANN et al., 2012; NEILL et al., 2014NEILL, J.D. et al. Complete genome sequence of pronghorn virus, a Pestivirus. Genome Announcements, v.2, n.3, p.1-2, 2014. Available from: <Available from: http://genomea.asm.org/content/2/3/e00575-14.full
>. Accessed: Jan. 13, 2016. doi: 10.1128/genomeA.00575-14.
http://genomea.asm.org/content/2/3/e0057...
; HAUSE et al., 2015HAUSE, B.M. et al. Discovery of a novel putative atypical porcine pestivirus in pigs in the USA. Journal of General, Virologyv.96, n.10, p.2994-2998, 2015. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/26219947
>. Accessed: Jan. 13, 2016. doi: 10.1099/jgv.0.000251.
http://www.ncbi.nlm.nih.gov/pubmed/26219...
; KIRKLAND et al., 2015KIRKLAND, P.D. et al. Genetic and antigenic characterization of Bungowannah virus, a novel pestivirus. Veterinary Microbiology, v.178, n.3-4, p.252-259, 2015. Available from: <Available from: http://www.sciencedirect.com/science/article/pii/S0378113515002102
>. Accessed: Jan. 13, 2016. doi:10.1016/j.vetmic.2015.05.014.
http://www.sciencedirect.com/science/art...
). However, we cannot rule out that the neutralizing antibodies detected were induced by other pestivirus infection than BVDV-1.
Multivariable logistic regression analysis showed an association between being housed outside and exposure to BVDV-1 (OR=0.24, 95% CI:0.06, 0.96, P=0.04). Herd size was found to be a confounder in this association and therefore was controlled in the model (OR=1.005, CI: 0.99-1.01, P=0.05). This association was no longer significant when accounting for the lack of independence of animals among farms using the GEE approach (Table 2).
Findings of multivariable logistic regression analysis were different of the expected since previous reports found that having other animal species such as bovine, goats and sheep in the same farm is a risk factor for infection by BVDV-1 (LOEFFEN et al., 2009LOEFFEN, W.L.A. et al. Seroprevalence and risk factors for the presence of ruminant pestiviruses in the Dutch swine population. Veterinary Microbiology, v.136, n.3-4, p.240-245, 2009. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/19128896
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2008.11.011.
http://www.ncbi.nlm.nih.gov/pubmed/19128...
). Occurrence of abortions, and other reproductive problems has also been previously reported to be associated with BVDV infection in pregnant cattle (BECHER et al., 2003BECHER, P. et al. Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. Virology, v.311, n.1, p.96-104, 2003. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/12832207
>. Accessed: Jun. 17, 2015. doi: 10.1016/S0042-6822(03)00192-2.
http://www.ncbi.nlm.nih.gov/pubmed/12832...
). Moreover, infected ruminants are considered the main infection source of BVDV for swines (DENG et al., 2012DENG, Y. et al. High prevalence of bovine viral diarrhea virus 1 in Chinese swine herds. Veterinary Microbiology, v.159, n.3-4, p.490-493, 2012. Available from: <Available from: http://www.ncbi.nlm.nih.gov/pubmed/22613254
>. Accessed: Jun. 17, 2015. doi: 10.1016/j.vetmic.2012.04.023.
http://www.ncbi.nlm.nih.gov/pubmed/22613...
).
Regarding the risk factor analysis, we were not able to associate any other variable with the disease occurrence. Considering the highly correlated data and low prevalence of antibodies at the animal level, there may have been insufficient power to detect true differences. The sample size was originally calculated with the objective of estimating prevalence; therefore, it did not necessarily meet the requirements for risk factor analysis.
In conclusion, anti-BVDV antibodies were detected in swine serum from Mossoró what could hinder the on going CSFV surveillance and eradication programs in the state of Rio Grande do Norte.
CONFLICT OF INTEREST
The authors declare that they have no competing interests.
BIOETHICS AND BIOSSECURITY COMMITTEE APPROVAL
The study was approved by Ethics Committee on Animal Use (CEUA) of Universidade Federal Rural do Semi-Árido, registered on N. 02/2013 and process N. 23091.003915/2012-42 CEUA/UFERSA.
ACKNOWLEDGEMENTS
The authors would like to acknowledge the farm owners and the laboratory staff of Departamento de Medicina Veterinária Preventiva e Reprodução Animal of Universidade Estadual Paulista "Júlio de Mesquita Filho" Câmpus de Jaboticabal, and Instituto Biológico do Estado de São Paulo. The financial support provided by grant 2014/13590-3, Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and for the scholarship by grant 132665/2014-0, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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» http://www.ncbi.nlm.nih.gov/pubmed/2848298
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1
CR-2015-1098.R4
Publication Dates
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Publication in this collection
07 June 2016 -
Date of issue
Sept 2016
History
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Received
31 July 2015 -
Accepted
14 Mar 2016 -
Reviewed
17 May 2016