In Barra de Guaratiba, an endemic area for American visceral leishmaniasis (AVL) in Rio de Janeiro, Brazil, control campaigns were unable to reduce canine infection rates. This difficulty prompted an in-depth study of dogs as a reservoir for Leishmania chagasi in the peri-urban environment through clinical and serological follow-up using the immunofluorescence and Western blot techniques. Recognition of 29kDa and 32kDa peptides by sera from dogs with proven L. chagasi infection was observed. Furthermore, only sera from symptomatic dogs recognized the 68.5kDa antigen, so the latter should be considered a parameter for culling dogs from endemic areas. The WB technique proved to be more sensitive than IFA, since the 29 and 32kDa peptide fractions were even recognized by sera from AVL seronegative dogs up to 8 months before IFA seroconversion. Proximity to wooded areas was an important risk factor for L. chagasi infection in dogs, possibly due to the presence of wild reservoirs.
Visceral Leishmaniasis; Dogs; Fluorescent Antibody Technique; Western Blot