Effect of <i>Clerodendranthus spicatus</i> (Thunb.) C. Y. Wu on the exercise ability of D-galactose-induced oxidative aging mice

WU, Jianzhong; CHEN, Hongjiang; WANG, Dan; ZHAO, Xin

doi:10.1590/fst.09822

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Original Article • Food Sci. Technol 42 • 2022 • https://doi.org/10.1590/fst.09822 copy

Effect of Clerodendranthus spicatus (Thunb.) C. Y. Wu on the exercise ability of D-galactose-induced oxidative aging mice

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Abstract

The Clerodendranthus spicatus (Thunb.) C. Y. Wu extract (CSTCYWE) was administered to D-galactose-induced aging mice to investigate the effect and mechanism of CSTCYWE on exercise endurance in aging mice. The effect of CSTCYWE on the improvement of exercise capacity in aging mice was evaluated by detecting serum biochemical indicators, pathological changes in tissues, and expression by qPCR. The composition of CSTCYWE was analyzed by HPLC. The experiment showed that CSTCYWE increased the running time and the exhaustive swimming time of aging mice to improve their exercise endurance. CSTCYWE also reduced the levels of BUN, BLA, and MDA and increased the levels of HG, MG, SOD, and GSH-Px in the serum of aging mice. Pathological observation found that CSTCYWE alleviated the liver and kidney tissue lesions and damage caused by aging. Meanwhile, CSTCYWE could down-regulate the relative mRNA expressions of nNOS, iNOS, TNF-α, and syncytin-1 and up-regulate the relative mRNA expressions of Cu/Zn-SOD, Mn-SOD, and CAT in the mouse liver tissue and skeletal muscle tissue. The results of component analysis showed that CSTCYWE contained caffeic acid, hypericin, isoquercetin, dihydroquercetin, rosmarinic acid, baicalin, luteolin, and baicalein. These active ingredients enable CSTCYWE to act on aging mice and improve their exercise capacity.

Keywords:
Clerodendranthus spicatus (Thunb.) C. Y. Wu; D-galactose; oxidative; exercise; mRNA

Gene	Forward sequence	Reverse sequence
Cu/Zn-SOD	5′-AACCAGTTGTGTTGTGAGGAC-3′	5′-CCACCATGTTTCTTAGAGTGAGG-3′
Mn-SOD	5′-CAGACCTGCCTTACGACTATGG-3′	5′-CTCGGTGGCGTTGAGATTGTT-3′
CAT	5′-GGAGGCGGGAACCCAATAG-3′	5′-GTGTGCCATCTCGTCAGTGAA-3′
NF-κB p65	5′-GAGGCACGAGGCTCCTTTTCT-3′	5′-GTAGCTGCATGGAGACTCGAACA-3′
nNOS	5′-TCGTCCAACTTCTGGGCTCTT-3′	5′-CCTTCTCTTCCTCCCCTCTCTTC-3′
iNOS	5′-CAAAGGCTGTGAGTCCTGCAC-3′	5′-ACTTTGATCAGAAGCTGTCCC-3′
TNF-α	5′-ATGGGGGGCTTCCAGAA-3	5′-CCTTTGGGGACCGATCA-3′
Syncytin-1	5′-GTTAACTTTGTCTCTTCCAGAATCGA-3′	5′-CATCAGTACGTGGGCTAGCA-3′
GAPDH	5′-TGACCTCAACTACATGGTCTACA-3′	5′-CTTCCCATTCTCGGCCTTG-3′

Group	Running time (s)	Load exhaustion swimming time (s)
Normal	52.63 ± 1.13^a	313.54 ± 14.25^a
Model	35.61 ± 1.36^e	195.42 ± 18.93^e
Vc	41.05 ± 1.23^c	254.71 ± 12.10^c
CSTCYWE-L	38.02 ± 0.79^d	218.91 ± 10.88^d
CSTCYWE-H	45.37 ± 1.41^b	278.12 ± 10.69^b

Group	BUN (mmol/L)	BLA (mmol/L)	MDA (mmol/mL)	HG (mg/mL)	MG (mg/mL)	SOD (U/mL)	GSH-Px (μmol/mL)
Normal	7.62 ± 0.45^e	21.03 ± 0.69^e	6.52 ± 0.41^e	11.02 ± 0.23^a	1.71 ± 0.08^a	336.75 ± 10.83^a	205.63 ± 8.32^a
Model	18.36 ± 0.61^a	38.33 ± 0.75^a	17.74 ± 0.62^a	7.11 ± 0.25^e	0.92 ± 0.04^e	121.07 ± 6.57^e	104.53 ± 5.60^e
Vc	12.51 ± 0.48^c	28.33 ± 0.35^c	10.32 ± 0.46^c	8.12 ± 0.22^c	1.31 ± 0.04^c	236.41 ± 11.08^c	142.69 ± 7.85^c
CSTCYWE-L	15.71 ± 0.39^b	31.25 ± 0.57^b	14.30 ± 0.55^b	7.63 ± 0.23^d	1.13 ± 0.05^d	167.85 ± 12.63^d	120.68 ± 6.91^d
CSTCYWE-H	10.30 ± 0.44^d	24.48 ± 0.39^d	8.06 ± 0.45^d	9.88 ± 0.31^b	1.50 ± 0.05^b	296.14 ± 8.91^b	177.20 ± 9.32^b

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[1] *Corresponding author: wuwang199709@163.com