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Effects of taro powder on the properties of wheat flour and dough

Abstract

This work aimed to investigate the effect of taro powder on the quality of wheat flour products. The water and starch content, whiteness, pasting properties, texture, microstructure, rheological properties of dough and water distribution in dough were analyzed. Taro powder significantly increased the peak viscosity, trough viscosity, final viscosity and setback value in the gelatinization process of flour, reduced the breakdown value, and increased the gelatinization temperature. Taro flour showed the ability to improve the farinographical properties and gelatinizing properties of flour. Adding taro flour reduces the development time and softness of the dough while increasing water absorption. The starch content, peak viscosity and water absorption of the mixed flour were the highest when the proportion of taro powder was increased to 25%. As the amount of taro powder increases, the color and hardness of the mixed flour dough become deeper, and the moisture inside the dough becomes less easily lost. The addition of taro powder may make the dough less resistant to mechanical blending and slightly less viscoelastic, but the gluten structure is smoother. In short, when the added amount of taro powder is 20% or 25%, it can meet the processing conditions.

Keywords:
taro powder; wheat flour; rheological properties; moisture state; microstructure

1 Introduction

Taro, an underground bulb of araceae plants, is mainly produced in Asia, Africa, Oceania and America. Currently, thousands of varieties of taro are cultivated worldwide (Aboubakar et al., 2010Aboubakar, N. Y. N., Nguimbou, R. M., Scher, J., & Mbofung, C. M. (2010). Effect of storage on the physicochemical, functional and rheological properties of taro (Colocasia esculenta) flour and paste. Innovative Romanian Food Biotechnology, 7, 37-48.; Aprianita et al., 2009Aprianita, A., Purwandari, U., Watson, B., & Vasiljevic, T. (2009). Physico-chemical properties of flours and starches from selected commercial tubers available in Australia. International Food Research Journal, 16, 507-520.). Taro is the main food for people living in African and Pacific Island countries. Mazon et al. (2020)Mazon, S., Menin, D., Cella, B. M., Lise, C. C., Vargas, T. de O., & Daltoé, M. L. M. (2020). Exploring consumers’ knowledge and perceptions of unconventional food plants: case study of addition of Pereskia aculeata Miller to ice cream. Food Science and Technology, 40(1), 215-221. http://dx.doi.org/10.1590/fst.39218.
http://dx.doi.org/10.1590/fst.39218...
reported that taro and roselle were the best known unconventional food plants (UFP) species for holistic and hedonic evaluation of the ice cream, but only 19.4% people stated they had the custom of eating these plants. Compared with other tubers and root plants, taro has a higher nutritional value. Taro is rich in starch and dietary fiber. The starch particle size of taro is small, and the average particle size of the different varieties of taro range from approximately 1-5.19 μm. Taro starch shows smooth and irregular shapes, from rounded shapes to polygons (Aboubakar et al., 2008Aboubakar, Njintang, Y. N., Scher, J., & Mbofung, C. M. F. (2008). Physicochemical, thermal properties and microstructure of six varieties of taro (Colocasia esculenta L.Schott) flours and starches. Journal of Food Engineering, 86(2), 294-305. http://dx.doi.org/10.1016/j.jfoodeng.2007.10.006.
http://dx.doi.org/10.1016/j.jfoodeng.200...
). The different sizes of taro starch granules may lead to differences in physical properties in the heating gelatinization process of taro starch, especially the absorbent and swelling power of taro starch (Adebowale & Lawal, 2004Adebowale, K. O., & Lawal, O. S. (2004). Comparative study of the functional properties of bambarra groundnut (Voandzeia subterranean), jack bean (Canavalia ensiformis) and mucuna bean (Mucuna pruriens) flours. Food Research International, 37(4), 355-365. http://dx.doi.org/10.1016/j.foodres.2004.01.009.
http://dx.doi.org/10.1016/j.foodres.2004...
). González Victoriano et al. (2021)Victoriano, L. G., Vera, N. G., Simental, S. S., Hernández, J. P., Lira, A. Q., & Martini, J. P. (2021). Quality properties of doughs and noodles using chayotextle (Sechiem edule) flours. Food Science and Technology (Campinas), 41(1), 158-166. http://dx.doi.org/10.1590/fst.30219.
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stated that the sticking properties of the starch play an important role in the quality of the product, because the starch provides the structure-forming properties. This behaviour is related to the macromolecules’ tendency to re-associate and interact after gelatinization, resulting in organized structures that delay starch swelling and solubilization during cooking. Taro is not only a good source of carbohydrates and dietary fiber but also acts a medicinal plant. Crude protein contents of taro flours ranged between 2–4%. Taro (Amadumbe) protein is rich in essential amino acids such as lysine (5.3 g/100 g) and leucine (9.1 g/100 g) (Hilary Van Wyk & Oscar Amonsou, 2021Hilary Van Wyk, R., & Oscar Amonsou, E. (2021). Physiochemical and functional properties of albumin and globulin from amadumbe (Colocasia esculenta) corms. Food Science and Technology. Ahead of Print. http://dx.doi.org/10.1590/fst.02621.
http://dx.doi.org/10.1590/fst.02621...
). Since ancient times, taro has been famous for its curative effects. It has medicinal purposes such as improving the stomach and intestines, nourishing the spleen and stomach, curing loose knots in the abdomen, and treating ailments such as swelling, psoriasis and injuries as a result of burns (Ahmed et al., 2010Ahmed, M., Akter, M. S., & Eun, J. B. (2010). Peeling, drying temperatures, and sulphite-treatment affect physicochemical properties and nutritional quality of sweet potato flour. Food Chemistry, 121(1), 112-118. http://dx.doi.org/10.1016/j.foodchem.2009.12.015.
http://dx.doi.org/10.1016/j.foodchem.200...
). In recent years, with the improvement of the dietary requirements of consumers, taro has been widely used in food processing, such as bread, cake, infant auxiliary food, etc. (Himeda et al., 2012Himeda, M., Njintang, N. Y., Nguimbou, R. M., Gaiani, C., Scher, J., Balaam, F., & Mbofung, C. M. F. (2012). Physicochemical, rheological and thermal properties of taro (Colocassia esculenta) starch harvested at different maturity stages. International Journal of Biosciences, 2(3), 14-27. ). Taro can improve the rheological properties and texture properties of food, while also giving it a good taste.

At present, the rheological properties and gelatinization properties of dough have received more attention at home and abroad. The texture of dough plays a decisive role in the sensory of quality in final products (González Victoriano et al., 2020González Victoriano, L., Güemes Vera, N., Soto Simental, S., Hernández, J. P., Quintero Lira, A., & Piloni Martini, J. (2020). Quality properties of doughs and noodles using chayotextle (Sechiem edule) flours. Food Science and Technology, 41(1), 158-166. http://dx.doi.org/10.1590/fst.30219.
http://dx.doi.org/10.1590/fst.30219...
). As a traditional staple food in China, noodles have a long history and occupy a very important position in the dietary structure of the population. In recent years, with the improvement of living standards, many consumers have increased requirements on the taste and quality of pasta. With traditional pasta no longer meeting the need of the population, more attention has been given to the nutritional value and safety of noodles (Tattiyakul et al., 2006Tattiyakul, J., Asavasaksakul, S., & Pradipasena, P. (2006). Chemical and physical properties of flour extractedfrom taro Colocasia esculenta (L.) schott grown in different regions of Thailand. Science Asia, 32(3), 279-284. http://dx.doi.org/10.2306/scienceasia1513-1874.2006.32.279.
http://dx.doi.org/10.2306/scienceasia151...
; Aboubakar et al., 2008Aboubakar, Njintang, Y. N., Scher, J., & Mbofung, C. M. F. (2008). Physicochemical, thermal properties and microstructure of six varieties of taro (Colocasia esculenta L.Schott) flours and starches. Journal of Food Engineering, 86(2), 294-305. http://dx.doi.org/10.1016/j.jfoodeng.2007.10.006.
http://dx.doi.org/10.1016/j.jfoodeng.200...
). Currently, a growing number of studies are evaluating the effects of wheat flour and alternative flour mixtures on the nutritional and functional properties of foods, such as quinoa flour and portulaca oleracea leaf powder. As expected, the dough and product quality were improved under certain dosage (Kurek & Sokolova, 2019Kurek, M. A., & Sokolova, N. (2019). Optimization of bread quality with quinoa flour of different particle size and degree of wheat flour replacement. Food Science and Technology, 40(2), 307-314. http://dx.doi.org/10.1590/fst.38318.
http://dx.doi.org/10.1590/fst.38318...
; Sadeghzadeh Benam et al., 2021Sadeghzadeh Benam, N., Goli, M., Seyedain Ardebili, S. M., & Vaezshoushtari, N. (2021). The quality characteristics of dough and toast bread prepared with wheat flour containing different levels of Portulaca oleracea leaf powder. Food Science and Technology. Ahead of Print. http://dx.doi.org/10.1590/fst.60820.
http://dx.doi.org/10.1590/fst.60820...
). In this study, wheat flour was used as the raw material in mixtures with taro flour at different proportions to make dough. By analyzing the physical and chemical properties of the mixed powder, as well as the thermodynamic and mechanical characteristics, texture characteristics, moisture distribution and microstructure of the dough, the processing quality of taro and wheat flour dough was obtained. It is expected to produce taro-wheat compound nutritional flour products with high nutritional value and good taste.

2 Materials and methods

2.1 Materials

The wheat flour used in this work was purchased from One Plus One Natural Flour Co., Ltd. (Xinxiang, China); fresh taro and salt were purchased from a local market (Xinxiang, Henan Province, China). All other reagents are analytically pure.

2.2 Preparation of the mixed flour

Fresh taro was selected, washed, peeled and cut into thin slices, dried at 40-45 °C, and crushed with a universal crusher through a 100-mesh sieve. Taro flour was mixed with wheat flour at proportions of 5%, 10%, 15%, 20%, 25% and 30%. Wheat flour without taro mixed in was used as the control.

2.3 Determination of whiteness of the mixed flour

The sample was put into a container of a certain volume, the surface was flattened, and the color of the mixed powder was determined by a cr-40 chromometer (Agama-Acevedo et al., 2011Agama-Acevedo, E., Garcia-Suarez, F. J., Gutierrez-Meraz, F., Sanchez-Rivera, M. M., Martin, E. S., & Bello-Perez, L. A. (2011). Isolation and partial characterization of Mexican taro (Colocasia esculenta L.) starch. Starch, 63(3), 139-146. http://dx.doi.org/10.1002/star.201000113.
http://dx.doi.org/10.1002/star.201000113...
). L* value represents brightness and whiteness, the larger the value, the whiter it is; A * means “red green degree”, the larger the value, the redder; B * stands for “yellow blue degree”, the larger the value, the more yellow.

2.4 Mixolab test

The pasting properties of dough were measured after adding water under the influence of both stirring force and temperature. Experimental instrument parameters: Chopin + experimental protocol; Dough weight: 75 g; Torque: 1.1 N.m (± 0.05 N.m); Speed: 80 r/min (Cashman & Flynn, 1999Cashman, K. D., & Flynn, A. (1999). Optimal nutrition: calcium, magnesium and phosphorus. The Proceedings of the Nutrition Society, 58(2), 477-487. http://dx.doi.org/10.1017/S0029665199000622. PMid:10466193.
http://dx.doi.org/10.1017/S0029665199000...
).

2.5 Rapid Viscosity Analyzer (RVA)

A RVA fast viscosimeter was used in the experiment. Three grams of mixing powder and 25 ml deionized water were weighed and mixed evenly in the aluminum box of the fast viscosity analyzer (Simsek & El, 2012Simsek, S., & El, S. N. (2012). Production of resistant starch from taro (Colocasia esculenta L. Schott) corm and determination of its effects on health by in vitro methods. Carbohydrate Polymers, 90(3), 1204-1209. http://dx.doi.org/10.1016/j.carbpol.2012.06.039. PMid:22939332.
http://dx.doi.org/10.1016/j.carbpol.2012...
). The following procedures were followed: the temperature was kept at 50 °C for 1 min, then the temperature was raised to 95 °C at a rate of 6 °C/min for 5 min, then the temperature was lowered to 50 °C at a rate of 6 °C/min for 2 min, the stirring rate within the first 10 s was 960 r/min, and then the viscosity was tested at a stirring rate of 160 r/min.

2.6 Texture Plus Analysis (TPA)

Structural characteristics of TPA (two chewing tests): By simulating the chewing motion of the human mouth, the solid and semisolid samples were compressed twice. Each sample dough with different amounts of taro powder added above was used for each measurement. The dough was pressed into 1 cm thick dough piece by a dough press, and then the dough pieces were cut into 10 cm × 10 cm pieces of dough (Luo et al., 2014Luo, Z., Li, D., Xie, J., Feng, S., & Wang, Y. (2014). Effects of heat treatment on quality and browning of fresh‐cut sugarcane. Journal of Food Processing and Preservation, 39(6), 688-696. http://dx.doi.org/10.1111/jfpp.12278.
http://dx.doi.org/10.1111/jfpp.12278...
). Using a P/36 probe to determine the dough texture. The parameters were: protest speed, 2 mm/s; test speed, 1 mm/s; postest, 1 mm/s, compression rate, 50%, trigger force 5 g. After determination, the texture of each dough was analyzed.

2.7 Low-field Nuclear Magnetic Resonance (LF-NMR)

Using the variable-temperature magnetic resonance imaging analyzer, the magnet is maintained at a constant temperature of 32 °C through the temperature control system (Dobraszczyk & Morgenstern, 2003Dobraszczyk, B. J., & Morgenstern, M. P. (2003). Rheology and the breadmaking process. Journal of Cereal Science, 38(3), 229-245. http://dx.doi.org/10.1016/S0733-5210(03)00059-6.
http://dx.doi.org/10.1016/S0733-5210(03)...
). The test parameters were: the magnet frequency was 20 MHz, the 90° pulse time P1 was 6.52 seconds, the 180° pulse time P2 was 13.04 seconds, the number of test samples TD = 300028, the monitoring sample frequency SW = 100 kHz, the sample detection interval TW = 5000, the number of repeats NS = 32, the analog gain RG1 = 20, the digital gain DRG1 = 3, the preamplifier PRG = 0.

2.8 Scanning Electron Microscopy (SEM)

An appropriate amount of dough was selected and frozen at -40 °C. Then, the central part of the frozen dough was cut into 1~2 mm slices with a razor blade and put into a freeze dryer for drying. After drying, the samples were fixed on the metal sample table with conductive double-sided adhesive tape, the impurities on the surface of the samples were brushed off by an ear washing ball, and the cross section was observed after vacuum spraying of palladium in the carrier chamber of an electron microscope. A total of 1500 and 2000 representations of each sample were used for observation, and the representative microstructure of the dough was photographed (Wang et al., 2013Wang, L., Xie, B. J., Xiong, G. Q., Wu, W. J., Wang, J., Qiao, Y., & Liao, L. (2013). The effect of freeze–thaw cycles on microstructure and physicochemical properties of four starch gels. Food Hydrocolloids, 31(1), 61-67. http://dx.doi.org/10.1016/j.foodhyd.2012.10.004.
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).

2.9 Statistical analysis

The data are presented as average values of at least three repeated measurements and were analyzed by one-way analysis of variance (ANOVA) and Duncan's tests (SPSS 16.0 Inc., Chicago, IL, USA). P < 0.05 was considered statistically significant.

3 Results and discussion

3.1 Color of the mixed powder

The effect of adding taro powder on the color of mixed flour was compared with the blank samples, which have higher L* values and whiter colors. The L* value of the pure taro powder is low, and the b value is also high, resulting in a darker color, such that the whiteness of the mixed powder gradually decreases with the increase with the addition on taro powder. Generally, when the whiteness index of flour is greater than 45, people's consumption and shelf life will not be affected (Belghith Fendri et al., 2016Belghith Fendri, L., Chaari, F., Maaloul, M., Kallel, F., Abdelkafi, L., Ellouz Chaabouni, S., & Ghribi-Aydi, D. (2016). Wheat bread enrichment by pea and broad bean pods fibers: effect on dough rheology and bread quality. Lebensmittel-Wissenschaft + Technologie, 73, 584-591. http://dx.doi.org/10.1016/j.lwt.2016.06.070.
http://dx.doi.org/10.1016/j.lwt.2016.06....
). As seen from Table 1, the brightness and whiteness of the mixed taro powder show a decreasing trend with increasing amount of added taro powder. The “red–green degree” varies unsteadily in the range of 0~20% of added taro powder but increases in the range of 20% ~ 30% of added taro powder. To ensure the nutritional value of taro powder in the mixture and that the whiteness of the mixture was not too low, 25% of the added amount was chosen to ensure the quality of the mixture and edible.

Table 1
Effect of taro powder on color and starch content of mixed powder.

3.2 Farinograph parameters by Mixolab

The dough stabilization time is an important index to reflect the kneading resistance of the dough and measure the characteristics of the flour. Generally, the longer the dough stabilization time is, the better the toughness of the dough, and the stronger the gluten strength is, the better the dough processing performance. Dough formation time is an important parameter for wheat flour components to absorb water to form dough and an important evaluation index for flour processing quality. In general, the longer the dough takes to form, the stronger the gluten and the better the dough (Mert et al., 2006Mert, B., Gonzalez, D., & Campanella, O. H. (2006). A new method to determine viscoelastic properties of corn grits during cooking and drying. Journal of Cereal Science, 46(1), 32-38. http://dx.doi.org/10.1016/j.jcs.2006.10.009.
http://dx.doi.org/10.1016/j.jcs.2006.10....
). The degree of protein weakening indicates the rate of destruction of the dough in the process of stirring, that is, the ability to withstand mechanical stirring, which represents the strength of the gluten. The greater the degree of weakening, the weaker the gluten, the easier the dough is to flow, and the poorer the operating performance.

From Table 2, with the increase of taro powder content, ranging from 0~30% taro powder,, the stable time change trend of mixed flour dough is relatively gentle, with little variation. The stable time had and the rub resistance of the dough mixed powder showed no clear change. This phenomenon may be associated with the nonstarch polysaccharides in taro powder. Water-soluble glue of nonstarch polysaccharides increased the gluten content and quality of the mixed powder, and the dietary fiber decreased the gluten content and quality of the nonstarch polysaccharide mixture flour. As shown in Table 2, the formation time of the dough gradually decreases as the increase of taro flour increases when the proportion of taro flour is in the range of 0~30%. Therefore, in the actual processing of taro flour noodles, from the point of view of the formation time of mixed flour dough, if the high dough formation time is required and the nutritional value of taro flour needs to be considered, then the appropriate amount of taro flour to be should range from 20% to 30% (Silva et al., 2012Silva, H. D., Cerqueira, M. Â., & Vicente, A. A. (2012). Nanoemulsions for food applications: development and characterization. Food and Bioprocess Technology, 5(3), 854-867. http://dx.doi.org/10.1007/s11947-011-0683-7.
http://dx.doi.org/10.1007/s11947-011-068...
).

Table 2
Test results of stabilization time, formation time and protein weakening degree of mixed flour dough with different amounts of taro powder.

According to the definition of Tamara, the C2 value in the monitoring map of Mixolab indicates the degree of protein weakening in the process of mechanical force and heating. The smaller C2 is, the greater the degree of protein weakening. From Table 2, it can be seen that with the increase in taro powder, the degree of weakening of the mixed flour shows a decreasing trend. C2 values of mixed flour decreases from 0.386 N.m to 0.283 N.m., indicating that adding taro powder to wheat flour will increase the degree of protein weakening of the mixed flour and reduce the evaluation value. The main reason is that taro flour does not contain gluten protein (Dvořáček et al., 2019Dvořáček, V., Bradová, J., Sedláček, T., & Šárka, E. (2019). Relationships among Mixolab rheological properties of isolated starch and white flour and quality of baking products using different wheat cultivars. Journal of Cereal Science, 89, 102801. http://dx.doi.org/10.1016/j.jcs.2019.102801.
http://dx.doi.org/10.1016/j.jcs.2019.102...
). With the addition of taro flour, the gluten characteristic of mixed flour is weakened, such that the gluten protein content in flour is relatively reduced, and the gluten network structure is poor compared with the raw wheat flour.

3.3 Pasting properties of dough by using mixolab

The difference value of C3-C2 in the spectrum of the Mixolab analyzer indicates the gelatinization characteristic of the starch, which is one of the indices to characterize the viscoelasticity of the dough. The larger the difference between C3-C2 is, the stronger the gelatinization characteristics of the starch and the larger the viscosity. As shown in Table 3, with the increase in additional taro powder, the difference between C3-C2 of the mixed powder decreased from 1.113 N.m to 0.888 N.m. The results showed that the starch gelatinization characteristics of the mixed taro powder decreased with the increase of added taro powder, the peak viscosity of the dough decreased gradually, the expansion degree of starch particles decreased, and the ability of the binding water decreased. This phenomenon may be due to the high amylose content in the starch of taro powder, and in starch gelatinization, the peak viscosity of the dough is negatively correlated with amylose content (Codină et al., 2019Codină, G. G., Istrate, A. M., Gontariu, I., & Mironeasa, S. (2019). Rheological Properties of Wheat-Flaxseed Composite Flours Assessed by Mixolab and Their Relation to Quality Features. Foods, 8(8), 333. http://dx.doi.org/10.3390/foods8080333. PMid:31395832.
http://dx.doi.org/10.3390/foods8080333...
). Therefore, with the addition of taro powder, the viscosity of the mixed paste tends to decrease. Starch gelatinization is essentially a process in which water molecules enter starch granules and the hydrogen bonds between starch molecules in the crystalline phase and amorphous phase break, destroying the associative state of starch molecules and dispersing it in water. It can be seen from Table 3 that the variation trend of the differences between C3-C4 is essentially the same, which increases at first and then decreases, indicating that the addition of taro powder reduces the thermal stability of starch paste of the mixed powder. However, when the content of taro powder reaches 15%, the difference between C3-C4 increases to 0.445 N.m, indicating that the starch gelatinization heat stability of the mixed powder is enhanced (Liu et al., 2014Liu, H., Nie, Y., & Chen, H. (2014). Effect of different starches on colors and textural properties of surimi-starch gels. International Journal of Food Properties, 17(7), 1439-1448. http://dx.doi.org/10.1080/10942912.2012.680224.
http://dx.doi.org/10.1080/10942912.2012....
). The difference between C5-C4 represents the reversibility of starch, which means that during the gradual cooling process of gelatinized starch, the molecular kinetic energy decreases, and the dense and highly crystallized starch molecular microbundles between adjacent molecules lose their solubility. If the value of C5-C4 is smaller, the starch is less likely to be recycled. As seen from Table 3, with the increase in the amount of taro powder added, the C5-C4 value of the mixed powder decreased from 0.647 N.m to -1.264 N.m. The decrease in the regeneration value indicates that the addition of taro powder improves the stability of cold paste and delays the regeneration of the flour products.

Table 3
Test results of Mixolab of starch mixed with different amounts of taro powder.

3.4 Pasting properties

Viscosity refers to the resistance of a fluid to flow in the same direction, that is, the internal friction between molecules in the fluid. The greater the internal friction between molecules, the greater the viscosity, resulting in a stronger gel strength. The pasting properties of the mixed powder were measured by a rapid viscosity analyzer (RVA) (Garimella Purna et al., 2011Garimella Purna, S. K., Miller, R. A., Seib, P. A., Graybosch, R. A., & Shi, Y. (2011). Volume, texture, and molecular mechanism behind the collapse of bread made with different levels of hard waxy wheat flours. Journal of Cereal Science, 54(1), 37-43. http://dx.doi.org/10.1016/j.jcs.2011.02.008.
http://dx.doi.org/10.1016/j.jcs.2011.02....
). As shown in Table 4, with increasing of taro powder content, the peak viscosity, peak time, valley viscosity, final viscosity and retrogradation value increase gradually. Grain viscosity represents the stability of cooking or hot paste of the broken starch at cooking temperature. The higher the grain viscosity is, the higher the stability of the mixed powder after heating. With the increase in taro powder content, the grain viscosity increases, and the stability of the mixed powder after heating also increases. The damage value is related to the strength of expanded starch grains, demonstrating the stability of the sample starch paste when heated. The smaller the damage value, the stronger the stability and shear resistance of the paste during the heating process, and the more stable it will be under thermal and mechanical action (Mohammed et al. 2012Mohammed, I., Ahmed, A. R., & Senge, B. (2012). Dough rheology and bread quality of wheat–chickpea flour blends. Industrial Crops and Products, 36(1), 196-202. http://dx.doi.org/10.1016/j.indcrop.2011.09.006.
http://dx.doi.org/10.1016/j.indcrop.2011...
). As seen from Table 4, with the increase in the amount of taro powder added, the damage value gradually decreases, and the stability of the mixed powder under thermal and mechanical effects increases. The ability of gelatinizing materials to form gels after cooling is determined by the final viscosity, which is affected by the retrogradation ability of the soluble amylose during the cooling stage. According to the analysis of the final viscosity, the higher the content of taro powder in the mixed powder, the higher the final viscosity, and the easier it is for the mixed powder to form the gel. The difference between the final viscosity and the valley viscosity is the retrogradation value, which is caused by the association of linear amylose molecules in the cooling stage and reflects the gluing capacity or retrogradation degree of the starch. The higher the retrogradation value, the worse the thermal stability of the gel, but the stronger the gel, meaning the more taro powder you have, the easier it is to harden and age during the cooling process (Charoenrein & Preechathammawong, 2012Charoenrein, S., & Preechathammawong, N. (2012). Effect of waxy rice flour and cassava starch on freeze–thaw stability of rice starch gels. Carbohydrate Polymers, 90(2), 1032-1037. http://dx.doi.org/10.1016/j.carbpol.2012.06.038. PMid:22840036.
http://dx.doi.org/10.1016/j.carbpol.2012...
). The gelatinization temperature is the lowest temperature at which the starch expands and breaks after gelatinization, reflecting the difficulty of gelatinization (Pu et al., 2017Pu, H., Wei, J., Wang, L., Huang, J., Chen, X., Luo, C., Liu, S., & Zhang, H. (2017). Effects of potato/wheat flours ratio on mixing properties of dough and quality of noodles. Journal of Cereal Science, 76, 236-242. http://dx.doi.org/10.1016/j.jcs.2017.06.020.
http://dx.doi.org/10.1016/j.jcs.2017.06....
; Zhu et al., 2001Zhu, J., Huang, S., Khan, K., & O’Brien, L. (2001). Relationship of protein quantity, quality and dough properties with chinese steamed bread quality. Journal of Cereal Science, 33(2), 205-212. http://dx.doi.org/10.1006/jcrs.2000.0358.
http://dx.doi.org/10.1006/jcrs.2000.0358...
). With the increase in the content of taro powder in the mixed powder, the gelatinization temperature increases gradually, and the peak time also increases, indicating that the higher the content of taro powder in the mixed powder is, the harder the gelatinization of the mixed powder.

Table 4
Effects of different amounts of taro powder on the viscosity of mixed flour.

When the added amount of taro powder was 25%, the peak viscosity was the highest, and the damage value was low. This indicates that the mixed powder containing 25% taro powder ha the strongest water absorption. When the amount of taro powder added was between 20% and 30%, the viscosity of the grain, the final viscosity and the retrogradation value were relatively high. This indicates that the viscosity and paste stability of the mixed taro powder in this range were significantly reduced, while the paste stability was improved (Zhang et al., 2011Zhang, S. B., Lu, Q. Y., Yang, H., & Meng, D. D. (2011). Effects of protein content, glutenin-to-gliadin ratio, amylose content, and starch damage on textural properties of chinese fresh white noodles. Cereal Chemistry, 88(3), 296-301. http://dx.doi.org/10.1094/CCHEM-05-10-0072.
http://dx.doi.org/10.1094/CCHEM-05-10-00...
).

3.5 TPA

Texture instruments are often used to evaluate some important quality indicators of dough. There are many test modes and parameters, such as hardness and cohesion in TPA mode, which are related to the sensory quality of dough. The higher the hardness number, the harder the dough. The higher the cohesion value is, the softer the dough (Wang et al., 2016Wang, X.-Y., Guo, X., & Zhu, K. (2016). Polymerization of wheat gluten and the changes of glutenin macropolymer (GMP) during the production of Chinese steamed bread. Food Chemistry, 201, 275-283. http://dx.doi.org/10.1016/j.foodchem.2016.01.072. PMid:26868577.
http://dx.doi.org/10.1016/j.foodchem.201...
). Table 5 shows that with the increase in taro powder, the hardness of the dough first increases, then decreases, and then increases slightly, in the range of 5% to 30%, with the lowest at 25%. The hardness of the dough is mainly determined by the denatured gluten network, and the more gluten in the dough, the higher the hardness. Compared with other groups, the increase in hardness when the additional amount of taro flour was in the range of 5% ~ 20% may be due to the increase in gluten network structure in the dough from the protein in taro flour. The lower hardness of the dough when taro powder is added at 25% is likely because taro powder destroys the gluten network structure, making it less rigid (Yoo & Jane, 2002Yoo, S., & Jane, J. (2002). Structural and physical characteristics of waxy and other wheat starches. Carbohydrate Polymers, 49(3), 297-305. http://dx.doi.org/10.1016/S0144-8617(01)00338-1.
http://dx.doi.org/10.1016/S0144-8617(01)...
). In addition, dough adhesiveness slowly reduced with the increase of adding amount of taro powder, which may be due to the low water imbibition of the taro powder; since it is not easy for water molecules to enter the interior of the starch granules when taro powder content is higher, the resulting dough adhesiveness increases.

Table 5
Effect of taro powder on dough texture.

3.6 LF-NMR

As shown in Figure 1, there are two peaks on each curve, representing two forms of water in the sample. Each sample T2 can be divided into two regions: T2 (P1) and T2 (P2). Among them, the peak value of T2 (P1) is between 4 and 7 ms, and the peak value of T2 (P2) is between 50 and 90 ms. The P1 region represents the water that binds with proteins, starch granules, sugars and other macromolecules and is called binding water (Kristiawan et al., 2018Kristiawan, M., Micard, V., Maladira, P., Alchamieh, C., Maigret, J., Réguerre, A.-L., Emin, M. A., & Della Valle, G. (2018). Multi-scale structural changes of starch and proteins during pea flour extrusion. Food Research International, 108, 203-215. http://dx.doi.org/10.1016/j.foodres.2018.03.027. PMid:29735050.
http://dx.doi.org/10.1016/j.foodres.2018...
). Most of the water in this region is not mobile. The P2 region represents the water exchanged with the gluten protein and starch particles and is located in the interstitium between the gluten protein network and the starch particles. The water in this region is the most easily flowing. Compared with the inverse relaxation curve of the dough without taro powder, the addition of taro powder did not increase the number of peaks of the dough relaxation curve but only changed the position of the peaks. The peaks of T2 (P1) and T2 (P2) moved significantly to the left, indicating that the binding water in the group behind the taro powder increased and the free water flow decreased (Zaidul et al., 2006Zaidul, I. S. M., Yamauchi, H., Kim, S., Hashimoto, N., & Noda, T. (2006). RVA study of mixtures of wheat flour and potato starches with different phosphorus contents. Food Chemistry, 102(4), 1105-1111. http://dx.doi.org/10.1016/j.foodchem.2006.06.056.
http://dx.doi.org/10.1016/j.foodchem.200...
).

Figure 1
Values from T2 spectrums of dough prepared with wheat flour-KGM blends.

3.7 SEM

Environmental scanning electron microscopy (ESEM) is a new electronic optical instrument developed in the past 30 years that can truly reflect the shape of the observed object. The effect of taro powder on the microstructure of dough is shown in Figure 2. In the dough without taro powder, the large and small starch particles were densely arranged and evenly distributed in the protein network. As the amount of taro powder added increased, the gluten network of the dough became looser, with small starch grains increasing and large starch grains decreasing and becoming more compact (Zarena et al., 2012Zarena, A. S., Bhattacharya, S., & Kadimi, U. S. (2012). Mangosteen oil-in-water emulsions: rheology, creaming, and microstructural characteristics during storage. Food and Bioprocess Technology, 5(8), 3007-3013. http://dx.doi.org/10.1007/s11947-011-0695-3.
http://dx.doi.org/10.1007/s11947-011-069...
). Other substances, such as nonstarch polysaccharides, in taro flour make the gluten structure smoother and have large stomata, but the structure is slightly looser. This indicates that the addition of taro powder may weaken the resistance to mechanical blending of the dough and that the viscoelasticity of the dough is slightly weaker. This may be because the starch grains of the taro powder are smaller, so the dough gathers more small molecules. There are many filamentous protein networks in wheat dough, but the filamentous protein network breaks after taro powder is added, which may be because some gluten proteins are destroyed after taro powder is added, resulting in loose deformation of the network structure (Kim et al., 2009Kim, Y., Huang, W., Zhu, H., & Rayas-Duarte, P. (2009). Spontaneous sourdough processing of Chinese northern-style steamed breads and their volatile compounds. Food Chemistry, 114(2), 685-692. http://dx.doi.org/10.1016/j.foodchem.2008.10.008.
http://dx.doi.org/10.1016/j.foodchem.200...
).

Figure 2
Microstructure of dough prepared with wheat flour-KGM blends (2000 ×). a, taro flour 0% (100% wheat flour); b, taro flour 5%; c, taro flour 10%; d, taro flour 15%; e, taro flour 20%; f, taro flour 25%; g, taro flour 30%.

4 Conclusions

With the increase in the amount of taro powder, the starch content of the mixed flour gradually increased, and the color gradually became darker. The water absorption of the mixed flour showed an upward trend, but the formation time of the dough and the weakening degree of the protein showed a downward trend. The properties of mixed roux and the thermal stability of starch paste tended to increase first and then decrease, and the stability of cold gelatinization of starch gradually decreased. The peak viscosity, peak time, grain viscosity, final viscosity and regaining value of the mixed powder increased gradually, while the damage value decreased gradually. Taro powder has a clear effect on the texture of dough. The addition of taro powder increases the firmness of the dough, but after a certain amount, the firmness decreases slightly. The cohesiveness of the dough increases with increasing taro powder. According to the transverse relaxation diagram, each sample curve has two peaks. The addition of taro powder causes both peaks of the dough to move to the left, reducing the moisture flow in the dough. The addition of taro powder may make the dough less resistant to mechanical blending and slightly less viscoelastic, but the gluten structure is smoother. Considering its nutritive factors and silty properties, it is suitable to make noodles when the proportion of taro flour in wheat flour is 25%.

  • Practical Application: Improvement the quality and nutrition of wheat dough blends with taro powder.

References

  • Aboubakar, N. Y. N., Nguimbou, R. M., Scher, J., & Mbofung, C. M. (2010). Effect of storage on the physicochemical, functional and rheological properties of taro (Colocasia esculenta) flour and paste. Innovative Romanian Food Biotechnology, 7, 37-48.
  • Aboubakar, Njintang, Y. N., Scher, J., & Mbofung, C. M. F. (2008). Physicochemical, thermal properties and microstructure of six varieties of taro (Colocasia esculenta L.Schott) flours and starches. Journal of Food Engineering, 86(2), 294-305. http://dx.doi.org/10.1016/j.jfoodeng.2007.10.006
    » http://dx.doi.org/10.1016/j.jfoodeng.2007.10.006
  • Adebowale, K. O., & Lawal, O. S. (2004). Comparative study of the functional properties of bambarra groundnut (Voandzeia subterranean), jack bean (Canavalia ensiformis) and mucuna bean (Mucuna pruriens) flours. Food Research International, 37(4), 355-365. http://dx.doi.org/10.1016/j.foodres.2004.01.009
    » http://dx.doi.org/10.1016/j.foodres.2004.01.009
  • Agama-Acevedo, E., Garcia-Suarez, F. J., Gutierrez-Meraz, F., Sanchez-Rivera, M. M., Martin, E. S., & Bello-Perez, L. A. (2011). Isolation and partial characterization of Mexican taro (Colocasia esculenta L.) starch. Starch, 63(3), 139-146. http://dx.doi.org/10.1002/star.201000113
    » http://dx.doi.org/10.1002/star.201000113
  • Ahmed, M., Akter, M. S., & Eun, J. B. (2010). Peeling, drying temperatures, and sulphite-treatment affect physicochemical properties and nutritional quality of sweet potato flour. Food Chemistry, 121(1), 112-118. http://dx.doi.org/10.1016/j.foodchem.2009.12.015
    » http://dx.doi.org/10.1016/j.foodchem.2009.12.015
  • Aprianita, A., Purwandari, U., Watson, B., & Vasiljevic, T. (2009). Physico-chemical properties of flours and starches from selected commercial tubers available in Australia. International Food Research Journal, 16, 507-520.
  • Belghith Fendri, L., Chaari, F., Maaloul, M., Kallel, F., Abdelkafi, L., Ellouz Chaabouni, S., & Ghribi-Aydi, D. (2016). Wheat bread enrichment by pea and broad bean pods fibers: effect on dough rheology and bread quality. Lebensmittel-Wissenschaft + Technologie, 73, 584-591. http://dx.doi.org/10.1016/j.lwt.2016.06.070
    » http://dx.doi.org/10.1016/j.lwt.2016.06.070
  • Cashman, K. D., & Flynn, A. (1999). Optimal nutrition: calcium, magnesium and phosphorus. The Proceedings of the Nutrition Society, 58(2), 477-487. http://dx.doi.org/10.1017/S0029665199000622 PMid:10466193.
    » http://dx.doi.org/10.1017/S0029665199000622
  • Charoenrein, S., & Preechathammawong, N. (2012). Effect of waxy rice flour and cassava starch on freeze–thaw stability of rice starch gels. Carbohydrate Polymers, 90(2), 1032-1037. http://dx.doi.org/10.1016/j.carbpol.2012.06.038 PMid:22840036.
    » http://dx.doi.org/10.1016/j.carbpol.2012.06.038
  • Codină, G. G., Istrate, A. M., Gontariu, I., & Mironeasa, S. (2019). Rheological Properties of Wheat-Flaxseed Composite Flours Assessed by Mixolab and Their Relation to Quality Features. Foods, 8(8), 333. http://dx.doi.org/10.3390/foods8080333 PMid:31395832.
    » http://dx.doi.org/10.3390/foods8080333
  • Dobraszczyk, B. J., & Morgenstern, M. P. (2003). Rheology and the breadmaking process. Journal of Cereal Science, 38(3), 229-245. http://dx.doi.org/10.1016/S0733-5210(03)00059-6
    » http://dx.doi.org/10.1016/S0733-5210(03)00059-6
  • Dvořáček, V., Bradová, J., Sedláček, T., & Šárka, E. (2019). Relationships among Mixolab rheological properties of isolated starch and white flour and quality of baking products using different wheat cultivars. Journal of Cereal Science, 89, 102801. http://dx.doi.org/10.1016/j.jcs.2019.102801
    » http://dx.doi.org/10.1016/j.jcs.2019.102801
  • Garimella Purna, S. K., Miller, R. A., Seib, P. A., Graybosch, R. A., & Shi, Y. (2011). Volume, texture, and molecular mechanism behind the collapse of bread made with different levels of hard waxy wheat flours. Journal of Cereal Science, 54(1), 37-43. http://dx.doi.org/10.1016/j.jcs.2011.02.008
    » http://dx.doi.org/10.1016/j.jcs.2011.02.008
  • González Victoriano, L., Güemes Vera, N., Soto Simental, S., Hernández, J. P., Quintero Lira, A., & Piloni Martini, J. (2020). Quality properties of doughs and noodles using chayotextle (Sechiem edule) flours. Food Science and Technology, 41(1), 158-166. http://dx.doi.org/10.1590/fst.30219
    » http://dx.doi.org/10.1590/fst.30219
  • Hilary Van Wyk, R., & Oscar Amonsou, E. (2021). Physiochemical and functional properties of albumin and globulin from amadumbe (Colocasia esculenta) corms. Food Science and Technology Ahead of Print. http://dx.doi.org/10.1590/fst.02621
    » http://dx.doi.org/10.1590/fst.02621
  • Himeda, M., Njintang, N. Y., Nguimbou, R. M., Gaiani, C., Scher, J., Balaam, F., & Mbofung, C. M. F. (2012). Physicochemical, rheological and thermal properties of taro (Colocassia esculenta) starch harvested at different maturity stages. International Journal of Biosciences, 2(3), 14-27.
  • Kim, Y., Huang, W., Zhu, H., & Rayas-Duarte, P. (2009). Spontaneous sourdough processing of Chinese northern-style steamed breads and their volatile compounds. Food Chemistry, 114(2), 685-692. http://dx.doi.org/10.1016/j.foodchem.2008.10.008
    » http://dx.doi.org/10.1016/j.foodchem.2008.10.008
  • Kristiawan, M., Micard, V., Maladira, P., Alchamieh, C., Maigret, J., Réguerre, A.-L., Emin, M. A., & Della Valle, G. (2018). Multi-scale structural changes of starch and proteins during pea flour extrusion. Food Research International, 108, 203-215. http://dx.doi.org/10.1016/j.foodres.2018.03.027 PMid:29735050.
    » http://dx.doi.org/10.1016/j.foodres.2018.03.027
  • Kurek, M. A., & Sokolova, N. (2019). Optimization of bread quality with quinoa flour of different particle size and degree of wheat flour replacement. Food Science and Technology, 40(2), 307-314. http://dx.doi.org/10.1590/fst.38318
    » http://dx.doi.org/10.1590/fst.38318
  • Liu, H., Nie, Y., & Chen, H. (2014). Effect of different starches on colors and textural properties of surimi-starch gels. International Journal of Food Properties, 17(7), 1439-1448. http://dx.doi.org/10.1080/10942912.2012.680224
    » http://dx.doi.org/10.1080/10942912.2012.680224
  • Luo, Z., Li, D., Xie, J., Feng, S., & Wang, Y. (2014). Effects of heat treatment on quality and browning of fresh‐cut sugarcane. Journal of Food Processing and Preservation, 39(6), 688-696. http://dx.doi.org/10.1111/jfpp.12278
    » http://dx.doi.org/10.1111/jfpp.12278
  • Mazon, S., Menin, D., Cella, B. M., Lise, C. C., Vargas, T. de O., & Daltoé, M. L. M. (2020). Exploring consumers’ knowledge and perceptions of unconventional food plants: case study of addition of Pereskia aculeata Miller to ice cream. Food Science and Technology, 40(1), 215-221. http://dx.doi.org/10.1590/fst.39218
    » http://dx.doi.org/10.1590/fst.39218
  • Mert, B., Gonzalez, D., & Campanella, O. H. (2006). A new method to determine viscoelastic properties of corn grits during cooking and drying. Journal of Cereal Science, 46(1), 32-38. http://dx.doi.org/10.1016/j.jcs.2006.10.009
    » http://dx.doi.org/10.1016/j.jcs.2006.10.009
  • Mohammed, I., Ahmed, A. R., & Senge, B. (2012). Dough rheology and bread quality of wheat–chickpea flour blends. Industrial Crops and Products, 36(1), 196-202. http://dx.doi.org/10.1016/j.indcrop.2011.09.006
    » http://dx.doi.org/10.1016/j.indcrop.2011.09.006
  • Pu, H., Wei, J., Wang, L., Huang, J., Chen, X., Luo, C., Liu, S., & Zhang, H. (2017). Effects of potato/wheat flours ratio on mixing properties of dough and quality of noodles. Journal of Cereal Science, 76, 236-242. http://dx.doi.org/10.1016/j.jcs.2017.06.020
    » http://dx.doi.org/10.1016/j.jcs.2017.06.020
  • Sadeghzadeh Benam, N., Goli, M., Seyedain Ardebili, S. M., & Vaezshoushtari, N. (2021). The quality characteristics of dough and toast bread prepared with wheat flour containing different levels of Portulaca oleracea leaf powder. Food Science and Technology Ahead of Print. http://dx.doi.org/10.1590/fst.60820
    » http://dx.doi.org/10.1590/fst.60820
  • Silva, H. D., Cerqueira, M. Â., & Vicente, A. A. (2012). Nanoemulsions for food applications: development and characterization. Food and Bioprocess Technology, 5(3), 854-867. http://dx.doi.org/10.1007/s11947-011-0683-7
    » http://dx.doi.org/10.1007/s11947-011-0683-7
  • Simsek, S., & El, S. N. (2012). Production of resistant starch from taro (Colocasia esculenta L. Schott) corm and determination of its effects on health by in vitro methods. Carbohydrate Polymers, 90(3), 1204-1209. http://dx.doi.org/10.1016/j.carbpol.2012.06.039 PMid:22939332.
    » http://dx.doi.org/10.1016/j.carbpol.2012.06.039
  • Tattiyakul, J., Asavasaksakul, S., & Pradipasena, P. (2006). Chemical and physical properties of flour extractedfrom taro Colocasia esculenta (L.) schott grown in different regions of Thailand. Science Asia, 32(3), 279-284. http://dx.doi.org/10.2306/scienceasia1513-1874.2006.32.279
    » http://dx.doi.org/10.2306/scienceasia1513-1874.2006.32.279
  • Victoriano, L. G., Vera, N. G., Simental, S. S., Hernández, J. P., Lira, A. Q., & Martini, J. P. (2021). Quality properties of doughs and noodles using chayotextle (Sechiem edule) flours. Food Science and Technology (Campinas), 41(1), 158-166. http://dx.doi.org/10.1590/fst.30219
    » http://dx.doi.org/10.1590/fst.30219
  • Wang, L., Xie, B. J., Xiong, G. Q., Wu, W. J., Wang, J., Qiao, Y., & Liao, L. (2013). The effect of freeze–thaw cycles on microstructure and physicochemical properties of four starch gels. Food Hydrocolloids, 31(1), 61-67. http://dx.doi.org/10.1016/j.foodhyd.2012.10.004
    » http://dx.doi.org/10.1016/j.foodhyd.2012.10.004
  • Wang, X.-Y., Guo, X., & Zhu, K. (2016). Polymerization of wheat gluten and the changes of glutenin macropolymer (GMP) during the production of Chinese steamed bread. Food Chemistry, 201, 275-283. http://dx.doi.org/10.1016/j.foodchem.2016.01.072 PMid:26868577.
    » http://dx.doi.org/10.1016/j.foodchem.2016.01.072
  • Yoo, S., & Jane, J. (2002). Structural and physical characteristics of waxy and other wheat starches. Carbohydrate Polymers, 49(3), 297-305. http://dx.doi.org/10.1016/S0144-8617(01)00338-1
    » http://dx.doi.org/10.1016/S0144-8617(01)00338-1
  • Zaidul, I. S. M., Yamauchi, H., Kim, S., Hashimoto, N., & Noda, T. (2006). RVA study of mixtures of wheat flour and potato starches with different phosphorus contents. Food Chemistry, 102(4), 1105-1111. http://dx.doi.org/10.1016/j.foodchem.2006.06.056
    » http://dx.doi.org/10.1016/j.foodchem.2006.06.056
  • Zarena, A. S., Bhattacharya, S., & Kadimi, U. S. (2012). Mangosteen oil-in-water emulsions: rheology, creaming, and microstructural characteristics during storage. Food and Bioprocess Technology, 5(8), 3007-3013. http://dx.doi.org/10.1007/s11947-011-0695-3
    » http://dx.doi.org/10.1007/s11947-011-0695-3
  • Zhang, S. B., Lu, Q. Y., Yang, H., & Meng, D. D. (2011). Effects of protein content, glutenin-to-gliadin ratio, amylose content, and starch damage on textural properties of chinese fresh white noodles. Cereal Chemistry, 88(3), 296-301. http://dx.doi.org/10.1094/CCHEM-05-10-0072
    » http://dx.doi.org/10.1094/CCHEM-05-10-0072
  • Zhu, J., Huang, S., Khan, K., & O’Brien, L. (2001). Relationship of protein quantity, quality and dough properties with chinese steamed bread quality. Journal of Cereal Science, 33(2), 205-212. http://dx.doi.org/10.1006/jcrs.2000.0358
    » http://dx.doi.org/10.1006/jcrs.2000.0358

Publication Dates

  • Publication in this collection
    14 Mar 2022
  • Date of issue
    2022

History

  • Received
    05 Nov 2021
  • Accepted
    14 Dec 2021
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