INFLUENCE OF THE NITROGEN LEVEL IN THE CULTIVATION OF Spirulina maxima IN TWO LEVELS OF TEMPERATURES: PART II: "PRODUCTION OF LIPIDS"

The micraolgae Spirulina maxima was cultivated in medium with 2,5g/L KNO3 (2,5N), proposed by Paoletti et al [10] and considered standard medium, in media with 0,2g/L KNO3 (0,2N) and in absence of nitrogen (0,0N) in temperatures of 25°C and 35°C. The composition of the dry cellular biomass presented to 35°C a medium increase of the total lipids, of 2,8 times of the middle with absence of nitrogen (0,0N) and 2,9 times of the half 0,2N in relation to the standard medium. To 25°C it presented a medium increase of 3,1 times of the medium with absence of nitrogen (0,0N) and 3,2 times of the half 0,2N in relation to the standard medium. The biomass in form of gel of each treatment was picked up and made the extraction of the total lipids, which were heavy in base evaporate. The statistical analyses of the results, in estimate non lineal with answer surface and multiple regression with analysis of residues, showed for the increase of the total lipids a lineal function with the temperature (T) and not lineal with the nitrogen (N), being adjusted to a model where the regression coefficient R=0,99459 and the equation LIPT = A+B²T+CN².

Spirulina; lipids; nitrogen; microalgae


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