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Determination of total protein and aminoacid composition of bromelain extracted from pineapple plant residues (Ananas comosus, (L.) Merril)

For a long time pineapple has been most popular noncitric fruit in tropical and subtropical countries, principally for its attractive flavor and aroma, besides refreshing sugar-acid balance, what certainly determined its social-economic importance. In addition to these characters, it is a source of the proteolytic bromelain enzyme , that is a high valued commercial product not made in Brazil. The present research has aimed in determination the aminoacids composition of extracted bromelain from some parts of the pineapple plant ( peel, leaf, stem, garland and pulp) with two subseqüents precipitation’s using ethanol p.a. as precipitating agent. Analysis showed higher protein content in extracted bromelain from the garland. In the two precipitation’s, the aminoacids composition from the obtained enzymes presented lower results than the one from the commercial enzyme.

protein; aminoacids; bromelain; pineapple plant


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