Therapeutic potential of hydrolysable tannin on weight management oxidative stress and reproductive health in polycystic rats

MANZOOR, Faiza; UN NISA, Mahr; HUSSAIN, Hafiz Amjad; ANWAR, Haseeb; AHMAD, Nazir; UMBREEN, Huma

doi:10.1590/fst.63720

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Original Article • Food Sci. Technol (Campinas) 42 • 2022 • https://doi.org/10.1590/fst.63720 copy

Therapeutic potential of hydrolysable tannin on weight management oxidative stress and reproductive health in polycystic rats

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Phytonutrients get attention now days due to their wide application and their tendency to improve the productive and reproductive performance in humans. Hydrolysable tannin (HT) is one of these bionutrients. The present study was aimed to investigate the therapeutic effect of HT on the induced polycystic ovarian syndrome (PCOS) in rats. Forty-five adult female rats with 4 to 5 days regular estrous cycle were selected. The PCOS was induced by a single intramuscular injection of Estradiol Valerate 4 mg/rat/kg. The rats were offered 0.5, 1, 1.5 and 2% of HT/kg body weight through oral gavage for two months. The results had shown a reduction (p<0.05) in average feed intake, body weight and weight gain, total oxidative stress and nutrient digestibility in PCOS rats. Similar reduction trend (p<0.05) in serum luteinizing hormone and insulin was observed; while serum follicular stimulating hormone, serum prolactin and total antioxidant capacity were improved (p<0.05). The ovarian histopathology showed no signs of reduction in cystic follicles while no improvement was observed in healthy follicles. The study concluded that the treatment with 1.5% level of HT had shown the best therapeutic potential for weight management, reduction of oxidative stress and improvement in reproductive health of PCOS rats.

Keywords:
polycystic ovarian syndrome; hydrolysable tannin; reproductive hormones; oxidative stress; histopathology

Ingredients	Amount (g/kg)
Corn Starch	230
Maltodextrine	100
Sucrose	100
Soya Bean Meal	420
Maize Bran	50
Soybean oil	50
Methionine	3
AIN 93G Vit Mix	10
AIN 93G Min Mix	35
Choline Bitartarate	2
Total Calories (kcal)	3822
Composition of Diet (g/kg) on Dry Matter basis (%)
Dry Matter	88.9
Moisture	11.1
Ash	8.3
Crude Fat	5.5
Crude Fiber	6.5
Crude Protein	18.6
Nitrogen Free Extract	50

Groups	PC0	PC0.5	PC1	PC1.5	PC2
Average Feed Intake (g)	22.02 ± 0.01^a	22.01 ± 0.03^a	21.96 ± 0.04^b	21.92 ± 0.05^b	21.86 ± 0.06^b
Initial Body Weight (g) (Before HT Treatment)	149.24 ± 3.75^a	148.20 ± 2.33^c	148.78 ± 2.84^b	148.89 ± 4.01^b	148.96 ± 2.09^b
Average Body Weight (g) (After 60 days treatment)	157.63 ± 1.33^a	156.61 ± 1.16^ab	154.83 ± 0.96^b	154.46 ± 1.20^bc	153.83 ± 0.90^c
Average Body Weight Gain (g)	17.75 ± 0.445^a	15.90 ± 0.465^b	10.14 ± 0.095^c	9.71 ± 0.118^c	7.99 ± 0.055^d

Groups	NC	PC0	PC 0.5	PC1	PC1.5	PC2
Insulin (µlU/mL)	15.01 ± 0.16^d	15.67 ± 0.28^a	15.57 ± 0.12^a	14.50 ± 0.13^c	14.42 ± 0.23^c	15.21 ± 0.20^b
LH (mlU/mL)	1.54 ± 0.01^c	2.68 ± 0.03^a	2.67 ± 0.03^a	2.24 ± 0.18^ab	1.96 ± 0.17^b	2.02 ± 0.12^b
FSH (mIU/mL)	2.56 ± 0.00^a	2.20 ± 0.03^c	2.27 ± 0.04^bc	2.32 ± 0.05^b	2.48 ± 0.02^a	2.42 ± 0.04^a
Progesterone (ng/mL)	15.61 ± 0.03^b	14.01 ± 0.15 ^a	14.05 ± 0.22 ^a	14.07 ± 0.13 ^a	14.04 ± 0.12 ^a	14.06 ± 0.10 ^a
Prolactin (ng/mL)	30.25 ± 0.01 ^a	30.28 ± 0.06 ^a	30.33 ± 0.06 ^a	30.33 ± 0.06 ^a	30.33 ± 0.06 ^a	30.40 ± 0.06^b
Estrogen (pg/mL)	33.77 ± 0.18^a	34.17 ± 0.16^b	34.15 ± 0.14^b	34.16 ± 0.14^b	34.16 ± 0.12^b	34.17 ± 0.12^b
Testosterone (ng/dL)	1.46 ± 0.00^b	2.67 ± 0.023 ^a	2.67 ± 0.06 ^a	2.67 ± 0.04 ^a	2.68 ± 0.061 ^a	2.68 ± 0.038 ^a

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[1] *Corresponding author: drfaizaahmadmmt@gmail.com