Homogenate extraction of crude polysaccharides from <i>Lentinus edodes</i> and evaluation of the antioxidant activity

KE, Leqin; CHEN, Haiyan

doi:10.1590/1678-457X.00916

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Research Paper • Food Sci. Technol (Campinas) 36 (3) • Jul-Sep 2016 • https://doi.org/10.1590/1678-457X.00916 copy

Homogenate extraction of crude polysaccharides from Lentinus edodes and evaluation of the antioxidant activity

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Crude polysaccharides of Lentinus edodes were extracted using homogenate method. Factors affecting the yield of crude polysaccharides were investigated and optimized by response surface methodology. The homogenate extraction method was compared with traditional heating extraction method. The antioxidant activity of crude polysaccharides from Lentinus edodes was evaluated. Results showed that, the optimal conditions of homogenate extraction were as follows: solvent pH, 10; liquid-solid ratio, 30: 1 (mL: g), extraction time, 66 s; number of extraction, 1. Under these conditions, the yield of crude polysaccharides was (13.2 ± 0.9)%, which was 29.82% higher than that of traditional heating extraction. Crude polysaccharides of Lentinus edodes had good DPPH scavenging activity. Compared with the traditional heating extraction, the homogenate extraction had notable advantages including good extraction yield, short extraction time and low extraction temperature. It is an efficient way to extract crude polysaccharides from Lentinus edodes.

Keywords:
crude polysaccharides; Lentinus edodes; homogenate extraction; response surface methodology

Variable	Level
Variable	-1	0	1
Solvent pH (X₁)	9	10	11
Liquid-solid ratio (mL: g) (X₂)	20:1	30:1	40:1
Extraction time (s) (X₃)	40	60	80

Run	Level			Variable			Yield of crude polysaccharides (%)
Run	X₁	X₂	X₃	Solvent pH	Liquid-solid ratio (mL: g)	Extraction time (s)	Yield of crude polysaccharides (%)
1	-1	-1	0	9	20	60	7.5 ± 0.5
2	1	-1	0	11	20	60	3.5 ± 0.7
3	-1	1	0	9	40	60	7.4 ± 1.3
4	1	1	0	11	40	60	2.0 ± 0.7
5	-1	0	-1	9	30	40	8.7 ± 1.1
6	1	0	-1	11	30	40	8.8 ± 0.3
7	-1	0	1	9	30	80	11.0 ± 0.8
8	1	0	1	11	30	80	2.8 ± 0.6
9	0	-1	-1	10	20	40	6.7±1.0
10	0	1	-1	10	40	40	10.9 ± 0.8
11	0	-1	1	10	20	80	6.4 ± 1.2
12	0	1	1	10	40	80	3.4 ± 0.7
13	0	0	0	10	30	60	12.5 ± 0.4
14	0	0	0	10	30	60	12.3 ± 0.9
15	0	0	0	10	30	60	14.4 ± 0.5
16	0	0	0	10	30	60	13.9 ± 1.1
17	0	0	0	10	30	60	12.1 ± 0.8

Source	Sum of Squares	df	Mean Square	F Value	Prob > F^a a P < 0.05 meant significant, and P < 0.01 meant highly significant.
Model	248.16	9	27.57	25.34	0.0002
X₁	38.46	1	38.46	35.34	0.0006
X₂	0.024	1	0.024	0.022	0.8857
X₃	16.36	1	16.36	15.03	0.0061
X₁X₂	0.45	1	0.45	0.41	0.5412
X₁X₃	17.31	1	17.31	15.90	0.0053
X₂X₃	12.67	1	12.67	11.64	0.0113
X₁ ²	51.23	1	51.23	47.07	0.0002
X₂ ²	84.05	1	84.05	77.23	<0.0001
X₃ ²	12.87	1	12.87	11.82	0.0109
Residual	7.62	7	1.09
Lack of Fit	3.44	3	1.15	1.10	0.4474
Pure Error	4.18	4	1.05
Cor Total	255.78	16	R² = 0.9702

Method	Extraction temperature	Extraction time	Solvent pH	Liquid-solid ratio (mL: g)	Number of extraction	Yield of crude polysaccharides (%)	P
A	Room temperature	66 s	10	30:1	1	13.2 ± 0.9	0.02
B	80 ^oC	2 h	9	30:1	2	10.2 ± 0.2	0.02

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[1] *Corresponding author: keleqin12@163.com