The aim of this paper is to study the absorption and cadmium (Cd) concentration by Saccharomyces cerevisiae, fermenting must of sugar-cane, with control contamination, under toxic levels of cadmium (mg Cd kg-1 must). The fermentation was performed in 500 mL erlemmayers. Non-sterilized must showed 12% of total reducing sugar (w/w) e pH 4,5. For the control contamination, was applied cadmium chloride, with four levels of contamination: 0,5; 1,0; 2,0 and 5,0 mg Cd kg-1 must. The inoculation of must was carried out with bread yeast (10% w/w). After fermentation (4 hours), samples were colected to evaluate cellular viability and yeast percentage. Fermenting mid was centrifuged and analysis of mid without yeast and raw yeast were performed. The alcohol content was measured , as well as the total humid weight for the yeast material, raw protein and heavy metal by atomic absorption spectroscopy. Watch all level studied have accumulation of cadmium at yeast.
heavy metal; cadmium; must of sugar-cane
