Effect of gelatin-based active coatings formulated with rosemary extract on quality of cold stored meatballs

Abstract

Meatballs were coated by gelatin-based mixes incorporated with potassium sorbate and rosemary extract. Coated samples were evaluated by free fatty acids (FFAs), thiobarbituric acid reactive substances (TBARS), and total volatile basic nitrogen (TVB-N) content. In addition, texture profile analysis (TPA) was performed to evaluate the textural features of meatballs. Uncoated sample as control along with 3 other samples coated by different formulations were stored in zipped plastic bags at +4 °C for 10 days. No significant difference was observed between samples regarding FFA and TBARS although coating with potassium sorbate showed suppressive effect on TVB-N initially. The starting pH of meatball was 6.22 and the coatings generally limited the change in pH compared to the control. Although significant differences in color were observed due to the color of coating itself, the coatings limited the variations in color to some extent. Other quality parameters were not improved much with the studied formulations. It is concluded that active edible coatings might be utilized in muscle foods for preservation and shelf-life extension if appropriately formulated and applied. Comprehensive studies are needed to determine the performance of carrier polymers in release of active ingredients and suitable formulations for every single group of food.

Keywords:
edible coating; gelatin; meatball; potassium sorbate; rosemary; quality

1 Introduction

Consumer demand has recently focused on healthy and bio-functional food although food safety and quality has always been an issue. There are many new methods currently under investigation to extend the shelf life and to preserve the foods with minimal processing. Keeping that in mind, edible coatings have gained some attention because of their potential use of carrying functional ingredients as well as active agents for creating healthy and bio-functional food formulations along with extended food quality (Aloui & Khwaldia, 2016; Díaz-Montes & Castro-Muñoz, 2021).

Nowadays, due to the increasing demand of consumers for safe products and the need to reduce food consumption, there has been an increase in the search for biodegradable packaging technologies that help reduce the negative impact of conventional packaging materials on the environment. In this context, the widespread use of edible films is particularly interesting (Fabra et al., 2018; Bojorges et al., 2020). Edible films can be used to protect food products by creating a “skin” on the products to protect them from microorganisms, chemicals or many different contamination sources available in the surrounding environment. Such films can mechanically protect food products, prevent the loss of water, volatile and flavor components, as well as reduce the level of food spoilage by controlling the passage of moisture, oxygen, and oxidizing agents (Kester & Fennema, 1989; Xie et al., 2002; Bravin et al., 2006). In addition, edible films are increasingly attracted because of their potential to improve sensory properties by providing a brighter and smoother appearance on the surface (Bravin et al., 2006).

Gelatin is an additive generally used as a thickening agent in the food industry. Its transparent, colorless, odorless and easily melting gel structure has not yet been achieved by other thickeners (Boran, 2011). Gelatin is also widely used in molecular gastronomy applications as a gelling ingredient as one of the rapidly spreading culinary trends (Ivanovic et al., 2011; Özdemir & Altıner, 2018). Gelatin-based edible coatings are materials in which gelatin is used as a carrier polymer and its mechanical strength is increased with the use of various plasticizers. The functional, organoleptic, nutritional and mechanical properties of gelatin-based edible coatings can be improved with various materials used in its production (Topuz & Boran, 2018). Compared to carbohydrate-based thickeners, gelatin has significant advantages in some aspects and is widely preferred due to its functional and technological advantages.

Meat has been an essential part of human diet since prehistoric times. In addition, it has a significant place among foods of animal origin in terms of nutritional value. Red meat is vital for mental and physical development due to its high protein content, not because of the quantity but quality i.e., it is rich and adequate in essential amino acids along with its high content of vitamin and minerals (Umaraw et al., 2020). Being highly sensitive to oxidation and various products including aldehydes, ketones, alcohols and other toxic compounds formed during oxidation may significantly limit the quality of meat and meat products. Meat is also very sensitive to microbiological deterioration and its shelf life is relatively short under inappropriate conditions (Bolumar et al., 2021). Post-mortem changes adversely affect quality parameters such as texture, color and taste, which reduce the shelf life and nutritional value of meat products. These problems, which pose a risk for consumers, also cause economic losses for producers (Bojorges et al., 2020). Edible films and/or coatings may conceptually help with preserving the composition and the nutritional value of meat, preventing moisture loss and transmission of volatiles; adding functionality to meat products, stabilizing the color and texture, reducing lipid oxidation, and extending the shelf life (Umaraw et al., 2020).

The use of edible films carrying antimicrobial and antioxidant ingredients is an active form of packaging that may contribute to shelf-life extension of food products and reduced safety risks for consumers. Organic acids, enzymes, fungicides, natural antimicrobial and antioxidant components are included in edible films for this purpose. Potassium sorbate (PS) is an antimicrobial food preservative that has been widely used for many years to stop or slow the growth of food pathogens and is generally considered as safe (Pranoto et al., 2005; Shen et al., 2010). Rosemary (Rosmarinus officinalis) is used as a natural preservative in foods due to its high antioxidant activity. The ingredients “1,8-cineol, α-pinene, camphor, camphor, borneol, piperitone, linalo” are the main components of rosemary essential oil. The antioxidant and antimicrobial effects of these main components have been reported by previous studies (Duman et al., 2012; Vital et al., 2016, 2018; Choulitoudi et al., 2017).

In this study, the effect of active coatings formulated with gelatin as a carrier polymer, sorbitol and glycerol as plasticizers, potassium sorbate as antimicrobial agent and rosemary extract as antioxidant ingredient on meatballs were investigated during cold storage at 4 °C for 10 days by following some chemical quality parameters and textural features during the storage. It was aimed to demonstrate that active coatings may be an appropriate method to ensure the quality and safety of meatballs sold as ready-to-use in markets and butchers.

2 Materials and methods

2.1 Materials

Minced beef meat and spices used for meatball mix were obtained from local markets. Bovine gelatin, potassium sorbate, glycerol, sorbitol and rosemary powder were obtained from local suppliers and of food grade. All ingredients were brought to the laboratory and the meatball dough was prepared as briefly described. Minced beef meat, as obtained from the butcher, was mixed with salt, onion, garlic, olive oil, egg, water and other spices. Formulation of meatball mix was 1500 g of minced meat (beef), 500 g of bread crumbs, 250 g of onion, 145 g of egg, 10 g of garlic, 30 g of olive oil, 10 g of salt, 5 g of red pepper flakes, 2 g of cumin and 2 g of black pepper. After kneading the mix, it was left to rest in the refrigerator for an hour. Then, the mix was portioned by hand in ice cube molds to shape. The flow chart of meatball production is given in Figure 1.

Figure 1
Flowchart of meatball production.

2.2 Sample preparation

The prepared meatballs were covered with active coating solutions by spraying. Meatballs were divided into 4 different groups including the control (uncoated) and 3 other samples coated by different coating formulations. Then, all 4 group of samples were placed in zipped bags and kept in the refrigerator at 4 °C for 10 days. All measurements were periodically performed at the beginning and on the 1st, 2nd, 3rd, 7th and 10th days of storage. All chemicals used were of analytical grade and obtained from Sigma-Aldrich (Missouri, USA) and Merck (Darmstadt, Germany). Formulation of edible coatings was composed of bovine gelatin (Gel) as carrier polymer; both sorbitol (Sor) and glycerol (Gly) as plasticizers; potassium sorbate (PS) and rosemary extract (RE) as antimicrobial and antioxidant agent, respectively. Stock solutions used in coating formulations are given below:

• Stc.1: 100 g of gelatin, 20 g of sorbitol, 10 mL of glycerol completed to 1 L by distilled water.

• Stc.2: 50 g of potassium sorbate completed to 500 mL by distilled water.

• Stc.3: 25 g of rosemary powder completed to 250 mL by distilled water. This mix was kept in a water bath at 50 °C for an hour with occasional stirring. Then, the mix was filtered through 4 layers of cheese cloth. This filtrate (about 200 mL) was completed to 250 mL by distilled water.

Coating solutions were obtained by different combinations of Stc.1, Stc.2 and Stc.3 solutions. Coating formulations and corresponding coding of the samples are given below:

• C (Control): No coating.

• PS: 100 mL of Stc.1 + 50 mL of Stc.2 + 50 mL of distilled water.

• RE25: 100 mL of Stc.1 + 50 mL of Stc.2 + 25 mL of Stc.3 + 25 mL of distilled water.

• RE50: 100 mL of Stc.1 + 50 mL of Stc.2 + 50 mL of Stc.3.

2.3 Methods

pH measurement

For measurement of pH, 2 g of homogenized meatball sample was mixed by 20 mL of distilled water. Then, pH value was measured using a pH meter (SG7, Mettler Toledo, OH, USA) (Soares et al., 2013).

Color analysis

Color parameters of L, a, and b were determined by a portable colorimeter (CSM5, PCE Instruments, Southampton Hampshire, UK). Measurements were performed on 5 different points of the surface (Chinnaswamy & Hanna, 1988). The change of the color in reference to the starting values before the storage was expressed as ∆E value and calculated according to the Formula 1 given below.

$\Delta E=\sqrt{{\left(L1–L2\right)}^2+{\left(a1–a2\right)}^2+{\left(b1–b2\right)}^2}$ (1)

Free fatty acids

Meatball samples were homogenized by a disperser (T18 Ultra-Turrax, IKA, Germany) at ambient temperature in the presence of n-hexane at a ratio of 1:2 (w/v). Then, the mix was filtrated through a coarse filter paper and the solvent was removed by a rotary evaporator. Approximately 0.5 g of oil sample was weighed and 25 mL of diethyl ether:ethanol (1:1, v/v) was added to dissolve the oil. 2-3 drops of 1% phenolphthalein indicator were added on this mix. Then, neutralization was achieved by titration by ethanolic solution of 0.1 M potassium hydroxide until a permanent pink color was observed. The amount of free fatty acids (FFAs) was calculated as oleic acid according to the Formula 2 given below (American Oil Chemists Society, 1989).

$$FFAs\left(\%oleicacid\right)=\left[\left(V-S\right)\times 2.805\right]/W$$ (2)

V: 0.1 M KOH used in titration of the sample (mL)

S: 0.1 M KOH used in titration of the blind (mL)

W: The amount of oil sample (g)

Conversion factor: 2.805

Total volatile basic nitrogen

Total volatile basic nitrogen (TVB-N) analysis was performed according to Olgunoğlu (2007). About 10 g of homogenized meatball sample was weighed into the kjeldahl tube. After adding 1 g of MgO and 100 mL of distilled water, it was placed in the distillation apparatus. 10 mL of 3% boric acid, 8 drops of methyl red and 100 mL of distilled water were added to the flask into where the distillate was collected. The instrument was operated until there was approximately 200 mL of distillate. The collected distillate was titrated with 0.1 N HCl and the consumption was recorded. TVB-N value was calculated according to the Formula 3 given below.

$$TVB-N\left(mgofTVB-Nin100gsample\right)=\left(V\times 1.4\times 100\right)/W$$ (3)

V: The amount of 0.1 N NaOH used in titration (mL)

W: The amount of the sample (g)

Thiobarbituric acid reactive substances

About 10 g of homogenized meatball sample was transferred into kjeldahl tubes and then 97.5 mL of distilled water and 2.5 mL of 4 N HCl solution were added to the tubes. Glass beads were added to prevent foaming during distillation. Distillation was continued until approximately 200 mL of distillate was obtained. 5 mL of the obtained distillate was transferred to a glass-capped test tube and 5 mL of 0.02 M TBA reagent was added. Mixing was performed by capping the tube and using a vortex. For the blank, 5 mL of TBA reagent and 5 mL of distilled water were added to another test tube and passed through the same process. These mixtures prepared in tubes were kept in a boiling water bath for 35 minutes, left to cool, and the absorbance was determined at a wavelength of 538 nm using a spectrophotometer. The amount of thiobarbituric acid reactive substances (TBARS) is calculated according to Formula 4 given below (Tarladgis et al., 1960).

$$TBARS\left(mgmalonaldehydesin100gsample\right)=Absorbance\times 7.8$$ (4)

Texture profile analysis

Texture Profile Analysis (TPA) was performed by using a texture analyzer (TA-XT II, Texture Technologies, USA) with a 125 mm diameter flat-surface cylindrical head. Meatball samples were prepared in 2 × 2 × 2 cm cubes and a TPA graph was obtained by applying 30% (6 mm) compression in both compression cycles. The compression speed was 1 mm/s, and the movement speed before and after the test was 2 mm/s. TPA parameters such as hardness, adhesiveness and springiness were calculated from the TPA graph obtained (Bourne, 2002).

2.4 Statistical analysis

The raw data obtained were organized as mean ± standard deviation of at least triplicate measurements. One-way ANOVA was utilized to determine if there is a significant difference between the means. Duncan test was used to determine which pairs are significantly different at a 95% confidence limit using SPSS program (SPSS Statistic 20, IBM, USA).

3 Results and discussion

3.1 Physical parameters

Change in pH values of coated samples is shown in Table 1. The initial pH of the sample was 6.22 ± 0.01. The pH of the control peaked on the 3rd day of storage but started to decrease afterwards. In case of coated samples, the pH constantly dropped during the storage. This is most likely due to the increase in concentration of organic acids because of the microbial and enzymatic activity. Enzymes from microorganisms that metabolize compounds in meat products will overhaul carbohydrates into lactic and acetic acid, which decreases the pH (Reynaldi et al., 2019). Microorganisms on meatballs may be originated from the surrounding environment, processing equipment and ingredients used in the formulation. Although the decreases in pH value were statistically insignificant (P>0.05), RE50 showed the most limited change in pH with an average value of 5.97 at the end of storage compared to other coated samples. This may be associated with the high antioxidant capability of rosemary extract used in the coating formulation.

Table 1
Change in color and pH of coated meatballs during storage.

Parameter	Sample	Initial	Day 1	Day 2	Day 3	Day 7	Day 10
L	C	48.50 ± 0.90Bab	49.98 ± 1.29Cb	49.31 ± 1.18Bab	46.01 ± 1.77Ba	49.31 ± 1.26Bab	48.83 ± 1.03Aab
	PS	47.04 ± 0.71ABa	47.94 ± 1.24BCa	46.90 ± 1.79ABa	43.56 ± 1.68Aa	46.84 ± 1.82ABa	46.53 ± 1.66Aa
	RE25	47.91 ± 1.15Ba	42.86 ± 1.89Aa	45.91 ± 1.43ABa	43.60 ± 1.79Aa	48.48 ± 1.64Ba	46.75 ± 1.72Aa
	RE50	45.51 ± 1.93Aa	45.24 ± 1.32ABa	42.71 ± 1.81Aa	43.24 ± 1.74Aa	40.82 ± 1.99Aa	44.09 ± 1.55Aa
a	C	10.26 ± 1.23Aab	8.77 ± 1.91Aa	9.99 ± 1.21Aab	11.36 ± 0.47Abc	12.56 ± 1.41ABc	13.04 ± 0.18Ac
	PS	9.91 ± 1.06Aab	8.12 ± 1.63Aa	10.67 ± 0.81Ab	11.45 ± 0.60Ab	11.08 ± 0.64Ab	12.15 ± 0.85Ab
	RE25	12.11 ± 1.17Aa	9.08 ± 1.74Aa	10.06 ± 1.32Aa	10.14 ± 1.77Aa	11.29 ± 0.86Aa	12.76 ± 0.93Aa
	RE50	9.94 ± 0.91Aab	9.56 ± 1.04Aa	11.28 ± 0.89Ab	10.68 ± 1.08Aab	13.21 ± 1.05Bc	13.43 ± 0.47Ac
b	C	25.56 ± 1.93Aa	21.26 ± 1.39Aa	24.13 ± 1.59Aa	25.79 ± 1.21Aa	26.05 ± 1.13Aa	25.50 ± 1.10Aa
	PS	25.67 ± 1.80Aa	24.65 ± 0.95Aa	28.55 ± 1.54Ba	27.49 ± 1.02Aa	25.61 ± 1.33Aa	27.85 ± 1.29Aa
	RE25	26.68 ± 1.86Aa	22.41 ± 1.17Aa	27.69 ± 1.21Ba	25.98 ± 1.54Aa	25.47 ± 1.39Aa	28.12 ± 1.15Aa
	RE50	26.47 ± 1.92Aa	26.20 ± 1.23Aa	28.26 ± 1.02Ba	25.00 ± 1.21Aa	26.69 ± 1.60Aa	27.54 ± 0.58Aa
∆E	C	-	7.18 ± 1.12Aa	3.89 ± 0.43Aa	4.58 ± 1.85Aa	5.09 ± 1.79Aa	4.57 ± 1.95Aa
	PS	-	6.12 ± 1.90Aab	3.51 ± 1.02Aa	4.42 ± 1.25Aab	3.71 ± 1.86Aa	7.15 ± 1.42Ab
	RE25	-	10.53 ± 1.22Aa	6.62 ± 1.93Aa	7.51 ± 1.55Aa	7.95 ± 1.31Aa	6.25 ± 1.34Aa
	RE50	-	4.51 ± 2.45Aa	4.32 ± 2.01Aa	5.27 ± 1.89Aa	7.45 ± 1.62Aa	4.61± 1.63Aa
pH	C	6.22 ± 0.01Ac	6.26 ± 0Be	6.24 ± 0.01Bd	6.24 ± 0.01Bd	5.98 ± 0.01Ab	5.71 ± 0.01Aa
	PS	6.26 ± 0Bd	6.25 ± 0.01Bd	6.22 ± 0.01ABcd	6.19 ± 0.03Ac	6.09 ± 0.04Bb	5.92 ± 0.04Ba
	RE25	6.27 ± 0.01Be	6.26 ± 0Bde	6.21 ± 0.01Acd	6.17 ± 0.02Ac	5.98 ± 0.05Ab	5.97 ± 0.05Ba
	RE50	6.28 ± 0Cc	6.24 ± 0.01Abc	6.21 ± 0.01Abc	6.16 ± 0.03Ab	6.03 ± 0.06ABa	5.90 ± 0.09Ba

• Values are given as mean ± standard deviation. Different lowercase letters in the same row indicate significant difference during storage while different uppercase letters in the same column indicate significant difference among samples separately for each parameter (P<0.05).

In previous studies, Reynaldi et al. (2019) examined the effect of garlic extract, which is added to a gelatin-based edible coating to preserve the meatballs and extend their shelf life. They observed that pH value of the coated samples was higher (pH 7.0-7.2 range) compared to the uncoated control while edible coatings prevented increase in the acidity. Pakpahan et al. (2019) investigated some of the quality parameters of meatballs coated by oleoresin-based coating. They determined that pH of uncoated control varied between 5.28 and 6.26 while pH of coated samples varied between 5.90 and 6.28 and pH varied significantly among the samples (P<0.05). Vital et al. (2018) examined the effect of an alginate-based edible coating containing natural antioxidants (rosemary and oregano essential oils) on beef stored for 14 days. In this context, some of its physical and chemical properties were checked during storage. It was concluded that there was no significant difference in pH values between the coated samples (P>0.05), but the pH was slightly higher in the control. Compared to Vital et al. (2018), in our study, it was determined that the pH value of the control is higher than the coated samples after 10 days of storage. In addition, it can be said that the pH value of the samples coated with rosemary in our study is higher than the pH value reported by Vital et al. (2018) at the end of the 7th day. This may be due to the concentration of rosemary in the coatings.

The color characteristics of meat and meat products increase the visual preference of the products. Color characteristics increase the attractiveness of foods and affect consumer choice (Kılınççeker, 2013). Changes in L, a, b and ΔE values of meatball samples during storage are given in Table 1. While the L value significantly indicates the brightness in all samples, the differences between samples are insignificant (P>0.05). In other samples, the L value decreased during storage, while the L value of the control sample increased at the end of storage. The positive a value indicating mild redness showed a statistically significant increase during cold storage (P<0.05). Also, at the end of storage (10th day), differences between samples were found to be significant (P<0.05). The positive b value indicating mild yellowness increased during storage, but this increase and the differences between samples were statistically insignificant (P>0.05). Changes occurred in ΔE value during storage. The color difference between the samples was compared with the samples analyzed on day 0. Visible color differences are detected in the coated meatballs and these differences continued during storage. Statistically significant fluctuations were found in the color difference between samples during storage (P<0.05). In color measurement, ΔE value indicates the color difference. If the ΔE value is greater than 2.3, there is a noticeable color difference. Looking at the results, it is seen that there is a noticeable color change in all samples. The color difference (∆E) given in Table 1 was calculated for a better evaluation of the color change in the samples. When the results were evaluated, the ∆E value decreased significantly (P<0.05) until the 7th day with the effect of potassium sorbate in the PS sample, but it peaked again on the 10th day. However, changes in ∆E value of other coated samples (samples RE25 and RE50) were not found to be significant (P>0.05). This indicates that all coating processes are successful in limiting the color change of meatballs during storage. In his study, Gerçek (2012) investigated the effect of coating with gelatin containing laurel and thyme oil on the quality of fish fillets stored at +4 °C for 15 days, and concluded that it has a positive effect on the color values. Antoniewski et al. (2007) investigated the effect of salmon fillets on shelf life by coating them with gelatin. It was concluded that the gelatin coating acts as a barrier to oxygen passage during storage, preventing the decrease in color quality of the fillets.

3.2 Free fatty acids

Free fatty acid content is a measure of quality deterioration of meat products and it is produced due to bacterial enzymatic lipolysis through the breakdown of fat into fatty acids and triglycerides (Khare et al., 2016). Changes in FFA amount during storage are given in Table 2. If it is necessary to make a general evaluation for the analysis results, the FFA amount of all samples increased during the storage period and these increases were found to be statistically significant (P<0.05). Similar to the change in color difference (ΔE) of sample PS, the amount of FFA remained lower than the other samples until the 7th day, and peaked on the 10th day. Accordingly, it was determined that the FFA amount of the PS sample was lower until the 7th day compared to the control group, and the coating had a slight effect. Although statistically significant differences were observed between the groups during the entire storage period (P<0.05), it was found that the control group had a lower FFA amount on the 10th day and the coating did not have a significant effect on FFA.

Table 2
Changes in the content of FFAs, TBARS and TVB-N during storage.

Parameter	Sample	Initial	Day 1	Day 2	Day 3	Day 7	Day 10
FFAs	C	0.90 ± 0.10Aa	0.97 ± 0.04Aa	1.23 ± 0.06Ab	1.24 ± 0.20Ab	1.52 ± 0.13Bc	1.43 ± 0.10Abc
	PS	0.95 ± 0.05Aa	1.18 ± 0.09Bb	1.28 ± 0.03Abc	1.42 ± 0.07Ac	1.32 ± 0.10Abc	1.62 ± 0.11Ad
	RE25	0.97 ± 0.06Aa	1.14 ± 0.02Bb	1.28 ± 0.04Ac	1.26 ± 0.06Abc	1.37 ± 0.06ABc	1.66 ± 0.14Ad
	RE50	1.00 ± 0.04Aa	1.18 ± 0.02Bb	1.23 ± 0.08Ab	1.23 ± 0.06Ac	1.49 ± 0.06Bcd	1.60 ± 0.12Ad
TBARS	C	0.30 ± 0.01Ade	0.32 ± 0.01Ae	0.26 ± 0.03Ac	0.22 ± 0.03Ab	0.12 ± 0.02Aa	0.29 ± 0.01Bd
	PS	0.37 ± 0.01Bb	1.09 ± 0.10Cc	0.31 ± 0Aab	0.28 ± 0ABab	0.28 ± 0.12Aa	0.26 ± 0.01Aab
	RE25	0.38 ± 0.01Bc	0.66 ± 0.01Bd	0.66 ± 0.02Bb	0.29 ± 0.02Cb	0.16 ± 0.03Aa	0.36 ± 0.01Cc
	RE50	0.37 ± 0.01Bc	0.31 ± 0Ab	0.31 ± 0.06Ab	0.38 ± 0.06Dc	0.19 ± 0.01Aa	0.36 ± 0Cbc
TVB-N	C	16.53 ± 0.94Aa	17.56 ± 1.42Aa	21.78 ± 1.70Ab	20.84 ± 1.31Ab	21.08 ± 1.17Ab	19.48 ± 1.04Aab
	PS	17.72 ± 0ABa	18.47 ± 0ABab	21.20 ± 1.08Abc	23.27 ± 1.13Ac	21.31 ± 1.21Abc	21.24 ± 1.21ABbc
	RE25	19.07 ± 0.81Ca	20.84 ± 0ABab	21.30 ± 0.47Aab	21.33 ± 1.42Aab	22.10 ± 1.57Ab	21.16 ± 0.81ABab
	RE50	21.19 ± 0.95Da	20.45 ± 1.24Ba	22.16 ± 0.77Aa	22.64 ± 1.02Aa	21.52 ± 1.68Aa	22.89 ± 1.04Ba

• Values are given as mean ± standard deviation. Different lowercase letters in the same row indicate significant difference during storage while different uppercase letters in the same column indicate significant difference among samples separately for each parameter (P<0.05).

Khare et al. (2016) observed changes in the quality parameters of chicken meat nuggets samples, on which cinnamon oil and chitosan enriched coatings were applied, during storage. The results showed that the FFA content increased significantly during storage and there was no significant difference between applications. In the study conducted by Gerçek (2012), it was determined that the samples coated with gelatin by adding laurel and thyme oil showed lower total volatile basic nitrogen, peroxide and free fatty acid values. Noor et al. (2018) investigated the effect of bioactive edible film containing Asparagus racemosus, which has antioxidant and antimicrobial effects, on meat quality. In edible films with antioxidant and antioxidant effects, lower FFA content was observed in all storage intervals compared to the control. Similarly, in our study, it was found that the amount of FFA in the coated samples remained lower until the 7th day. However, on the 10th day, the amount of FFA increased compared to the control. This may indicate that the effect of the coating on the samples is about 7 days.

3.3 Thiobarbituric acid reactive substances

Microbial spoilage along with chemical deterioration especially lipid oxidation is considered as one of the main factors limiting the shelf life of muscle foods (Khare et al., 2016). Lipid peroxidation was measured in terms of thiobarbituric acid (TBA) and was given in Table 2. It has been observed that TBA value increases and decreases during storage. When the results were compared, statistical differences were found between the samples (P<0.05), but these differences were not positively realized. The TBA value of group PS progressed at lower levels on the 10th day compared to the control. However, other groups in which the coating application was applied reached higher TBA values during storage compared to the control group.

In the study conducted by Song et al. (2011), the effect of edible coating containing different antioxidants on the quality and shelf life of cooled sea bream fish (Megalobrama amblycephala) was investigated. It was observed that the TBA values of all samples increased continuously during storage. However, it was determined that the TBA value of the uncoated samples was significantly lower (P<0.05). This showed that the coating containing antioxidant effectively prevents lipid oxidation. The study by Ruan et al. (2019) investigated the effect of edible coating on fresh pork quality and shelf life. TBA values of samples were measured as lipid oxidation index and it was determined that it increased continuously during storage. Compared to the uncoated samples, it was found that the TBA values of the coated samples decreased significantly during storage (P<0.05). In our study, it was determined that the TBA value of the PS sample at the end of storage was lower than the control. This may be due to the antimicrobial effect of potassium sorbate.

3.4 Total volatile basic nitrogen

Meat freshness is very important for acceptance by end-consumers. However, the widespread adoption of TVB-N for interpretation of freshness is more limited in meats than in seafood. TVB-N is produced by the degradation of proteins and amines. The pathways of its formation are related to the activity of endogenous enzymes and disrupting bacteria on methylated amines. As a result, TVB-N content generally increases with meat products storage times, and this increase is consistent with other degradation biomarkers (Bekhit et al., 2021). When the results determined by us are examined, TVB-N values increased with the progress of storage and these increases occurred regularly on some days and irregularly on some days. These increases were found to be statistically significant (P<0.05). While the TVB-N value of the control group progressed at lower levels, the coated samples were found to have higher levels of TVB-N compared to the control.

In the study conducted by Andevari & Rezaei (2011), the effect of coating with cinnamon oil containing gelatin on quality during storage of trout fillet in refrigerator conditions (4 ± 1 °C) for 20 days was investigated. In the study, it was observed that gelatin films containing cinnamon oil decreased the TVB-N level. Kim et al. (2018) investigated the effect of edible antimicrobial coating on extending the shelf life of shrimp (Litopenaeus vannamei) stored at 4 °C. The measured TVB-N value as an indicator of decay increased significantly during storage in all uncoated and coated samples (P<0.05). However, the increase in the amount of TVB-N in the samples coated with antimicrobial film was found to be rather limited compared to the control. TVBN value was observed during storage in the study conducted by Ruan et al. (2019) in which the effect of edible coating on pork quality was investigated. TVBN value increased during storage, but it was determined that this increase was limited in coated samples. The coating is thought to act as an oxygen barrier on samples, restricting oxidation and protein degradation caused by microorganisms.

3.5 Textural features

TPA results are given in Table 3. It is seen that hardness was lower in all coated samples at the end of the 10th day of storage compared to that of the control. Apparently, the coatings showed a softening effect. Cohesiveness was the highest in the control and the lowest in the PS sample although these differences were not statistically significant (P>0.05). The adhesiveness value of all coated samples is very low compared to the control. The lowest adhesiveness value was determined in the PS25 sample. The differences observed in the adhesiveness values were found to be statistically significant (P<0.05). The highest spring rate was found in PS25 sample (0.68%) and lowest in RE sample (0.55%). The differences observed in springing values were also found to be statistically significant (P<0.05). At the end of storage, it was determined that the highest chewiness and gumminess values were in the control sample. The lowest chewiness was detected in the RE sample (219.34) and the lowest gumminess was detected in the PS25 sample (261.05). When evaluated in general, it was seen that the hardness, cohesiveness, adhesiveness, chewiness and gumminess values of the control sample were higher at the end of storage. It was found that the adhesiveness and springiness state were better in the coated samples. It was determined that the coating at the end of storage prevents the adhesiveness of the samples from increasing and retains the flexibility, especially in the RE sample.

Table 3
Textural properties of the samples.

	Sample	Initial	Day 1	Day 2	Day 3	Day 7	Day 10
Hardness	C	701.96 ± 54.74Ab	612.43 ± 74.72Ab	697.60 ± 135.80Aa	815.77 ± 110.05ABa	1098.67 ± 79.52Ca	993.76 ± 299.98BCa
	PS	641.30 ± 43.23Aab	633.46 ± 20.57Ab	576.63 ± 38.01Aa	603.53 ± 30.29Aa	1150.95 ± 399.44Ba	744.68 ± 44.05Aa
	RE25	587.22 ± 44.47Ba	457.87 ± 8.37Aa	746.33 ± 80.52Ca	664.34 ± 48.45BCa	870.45 ± 40.78Da	674.74 ± 54.70BCa
	RE50	718.48 ± 70.42Ab	619.62 ± 120.32Ab	580.32 ± 100.65Aa	849.76 ± 235.49Aa	724.50 ± 135.85Aa	695.16 ± 138.20Aa
Cohesiveness	C	0.50 ± 0.01Aa	0.57 ± 0.07Aa	0.73 ± 0.37Aa	0.50 ± 0.01Aa	0.47 ± 0.02Aa	0.87 ± 0.40Aa
	PS	0.59 ± 0.05Aab	0.57 ± 0.01Aa	0.59 ± 0.00Aa	0.53 ± 0.06Aa	0.52 ± 0.05Aab	0.54 ± 0.01Aa
	RE25	0.65 ± 0.08ABb	0.66 ± 0.04Aba	0.73 ± 0.18Ba	0.60 ± 0.07ABa	0.55 ± 0.05Ab	0.57 ± 0.02ABa
	RE50	0.60 ± 0.01Aab	0.77 ± 0.31Aa	0.62 ± 0.02Aa	0.59 ± 0.04Aa	0.60 ± 0.03Ab	0.63 ± 0.02Aa
Adhesiveness	C	-134.45 ± 7.95Aa	-399.74 ± 110.85Ba	-426.94 ± 52.75Bb	-346.43 ± 2.66Bc	-309.18 ± 3.84Bb	-627.15 ± 89.47Cd
	PS	-144.53 ± 25.66Aa	-406.57 ± 4.57Ea	-353.04 ± 19.05Db	-277.96 ± 3.82Bb	-288.16 ± 1.53Bb	-317.53 ± 13.87Cb
	RE25	-311.02 ± 9.29Cb	-401.45 ± 20.76Da	-251.08 ± 38.73Ba	-133.84 ± 41.36Aa	-137.97 ± 15.93Aa	-132.22 ± 5.90Aa
	RE50	-352.55 ± 34.99BCb	-399.04 ± 27.30BCa	-174.96 ± 69.37Aa	-264.37 ± 22.33ABb	-331.81 ± 154.24BCb	-426.47 ± 46.39Cc
Springiness	C	0.54 ± 0.01Aa	0.60 ± 0.08ABa	0.57 ± 0.04ABa	0.58 ± 0.01ABa	0.55 ± 0.01ABa	0.62 ± 0.02Bb
	PS	0.58 ± 0.02Aba	0.62 ± 0.01Ba	0.59 ± 0.05ABa	0.60 ± 0.02Ba	0.60 ± 0.01Bb	0.55 ± 0.01Aa
	RE25	0.75 ± 0.02Dc	0.69 ± 0.03BCa	0.73 ± 0.03CDb	0.62 ± 0.03Aa	0.65 ± 0.01ABc	0.68 ± 0.03BCc
	RE50	0.66 ± 0.05ABb	0.63 ± 0.03ABa	0.71 ± 0.02Bb	0.69 ± 0.06Bb	0.67 ± 0.02ABc	0.60 ± 0.05Aab
Chewiness	C	190.11 ± 10.24Aa	212.44 ± 57.42Aa	282.06 ± 123.85ABab	236.49 ± 28.29Aa	286.46 ± 37.61ABa	589.09 ± 35.67Ba
	PS	219.90 ± 27.45Aa	222.44 ± 6.77Aa	199.35 ± 21.26Aa	195.00 ± 31.53Aa	357.03 ± 100.1Ba	219.34 ± 17.70Aa
	RE25	286.78 ± 34.14Bb	207.26 ± 19.77Aa	397.17 ± 80.93Cb	245.69 ± 28.78ABa	315.08 ± 26.96Ba	261.05 ± 24.54ABa
	RE50	284.51 ± 40.28Ab	312.29 ± 174.71Aa	252.41 ± 43.72Aab	335.28 ± 51.99Ab	287.86 ± 32.77Aa	263.21 ± 25.52Aa
Gumminess	C	349.63 ± 22.43Aa	350.52 ± 55.79Aa	491.22 ± 199.25ABa	408.85 ± 53.48Aab	518.05 ± 57.13ABa	942.11 ± 60.51Ba
	PS	378.40 ± 58.19Aa	357.74 ± 3.33Aa	340.92 ± 24.22Aa	322.80 ± 45.28Aa	593.88 ± 168.06Ba	400.97 ± 31.57Aa
	RE25	382.50 ± 53.62ABa	301.51 ± 21.55Aa	542.05 ± 104.81Ca	398.57 ± 54.01ABab	482.38 ± 41.97BCa	382.16 ± 29.32ABa
	RE50	428.03 ± 50.76Aa	497.68 ± 294.55Aa	356.24 ± 51.34Aa	491.10 ± 110.47Ab	430.30 ± 59.42Aa	439.73 ± 77.28Aa

• Values are given as mean ± standard deviation. Different uppercase letters in the same row indicate significant difference during storage while different lowercase letters in the same column indicate significant difference among the samples separately for each parameter (P<0.05).

Tosati et al. (2017) examined the effect of edible film, which is an antimicrobial agent carrier, on the textural properties of sausages. At the end of storage at 10 °C for 30 days, it was determined that the hardness and springiness values were higher in the uncoated sample, just as in our study. The cohesiveness value was almost the same in the coated and uncoated samples, while the chewiness value was significantly higher in the coated sample (P>0.05). In the study conducted by Gallego et al. (2020), the effect of gelatin coating enriched with antioxidant tomato by-products on pork quality was investigated. At the end of storage at 4 °C for 13 days, unlike our study, the hardness, cohesiveness and chewiness values were determined in the coated samples with the highest, elasticity in the best control and the highest adhesiveness values in the control, as in our study. In the study by Piedrahíta Márquez et al. (2019), fish fillets were treated with an edible coating enriched with antioxidant and antimicrobial substances. When the results of the TPA analysis performed at the end of 20 days of cold storage were examined, it was found that while adhesiveness increased, hardness and gumminess values decreased. At the end of storage, it was determined that the springiness was the highest in the coated samples, as in our study.

4 Conclusions

This study is among few studies on use of edible coatings for muscle foods. Although the results are not strongly conclusive, promising effects have been observed in terms of color preservation, suppressed protein deterioration and improved textural quality. Based on the results, it may be concluded that edible active coatings may help with the preservation of meatballs and extension of shelf life when applied properly in product-specific formulations. It was observed that coating with potassium sorbate (PS) showed a significant suppressive effect on TVB-N formation during storage. In addition, the coatings led to limited changes in pH values. Considering the TPA results, it was observed that hardness and stickiness were lower and the springiness was higher in coated samples compared to the control. It is, therefore, concluded that coating formulations studied resulted in improved textural quality. Significant differences were observed in color due to the color of coating itself but the coatings, in general, limited the variations in color to some extent. Further research is needed to determine proper formulation of edible coatings that may work for each group of food. In future research, different bioactive ingredients and/or functional compounds may be incorporated with various carrier polymers to develop effective edible coatings.

• Practical Application: The presented work concludes that edible and active coatings may successfully be used in preservation of muscle foods. However, further studies are needed to optimize such formulations for superior release of active ingredients and also, for better preservation of the quality. Nevertheless, meat and seafood processing industry may find the data presented in this study useful and utilize edible and active coatings for shelf-life extension and/or for improving the nutritional and bioactive value of such products.

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Publication Dates

• Publication in this collection
  28 June 2021
• Date of issue
  2022

History

• Received
  27 Apr 2021
• Accepted
  13 May 2021