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A retrospective study: physicochemical properties of the flower honey from the Black Sea region of Turkey in different years

Abstract

Honey has a remarkable commercial value as a natural product for producers. This study aimed to examine the differences in the physicochemical properties of the flower honey produced in Kastamonu, a province in the Black Sea Region of Turkey, in different years to see if they changed and if the physicochemical properties met the quality standards for honey. To this end, the honey samples (Apis mellifera) produced in Kastamonu province were studied. The samples were collected from 40 producers of honey between 2016 and 2019. We carried out the quality analysis according to the Turkish Food Codex Communiqué on Honey and compared it with the EU standards. The results showed that fructose/glucose ratio, total fructose and glucose (%), diastase (shade), proline (mg/kg), electrical conductivity (ms/cm), HMF (mg/kg), and acidity (meq/kg) did not change during four years while the moisture content differed significantly. In other words, the quality of the studied samples remained stable in four years. The majority of the studied honey samples obtained from the Black Sea Region of Turkey were compatible with the Turkish Food Codex Communiqué on Honey and the EU standards.

Keywords:
Black Sea Region; honey; physicochemical parameters; quality; Turkey

1 Introduction

Honey is consumed all over the world for therapeutic, nutritional, and medicinal purposes (Yaqub et al., 2020Yaqub, G., Khalid, M., Ikram, A., & Sohail, A. (2020). Monitoring and risk assessment due to presence of metals and pesticides residues in honey samples from the major honey producing forest belts and different brands. Food Science and Technology, 40(Suppl. 1), 331-335. http://dx.doi.org/10.1590/fst.01919.
http://dx.doi.org/10.1590/fst.01919...
). Honey is a natural product of honey bees (Apis mellifera) used for different purposes since ancient times (Ajibola et al., 2012Ajibola, A., Chamunorwa, J. P., & Erlwanger, K. H. (2012). Nutraceutical values of naturalhoney and its contribution to human health and wealth. Nutrition & Metabolism, 9(1), 61. http://dx.doi.org/10.1186/1743-7075-9-61. PMid:22716101.
http://dx.doi.org/10.1186/1743-7075-9-61...
) and is produced from the nectar collected by worker bees from flowering plants, and then transformed, dehydrated, and stored in honeycombs (Krell, 1996Krell, R. (1996). Value-added products from beekeeping (FAO Agricultural Services Bulletin, No. 124). Rome: Food and Agriculture Organization of the United Nations.). Honey is an excellent food supply that includes sugars, other nutrients, and phytochemicals such as vitamins, minerals, amino acids, organic acids, phenolics, and aromatic compounds (Silva et al., 2016Silva, P. M., Gauche, C., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Honey: chemical composition, stability and authenticity. Food Chemistry, 196, 309-323. http://dx.doi.org/10.1016/j.foodchem.2015.09.051. PMid:26593496.
http://dx.doi.org/10.1016/j.foodchem.201...
; Singh & Bath, 1997Singh, N., & Bath, P. K. (1997). Quality evaluation of different types of Indian honey. Food Chemistry, 58(1), 129-133. http://dx.doi.org/10.1016/S0308-8146(96)00231-2.
http://dx.doi.org/10.1016/S0308-8146(96)...
).

Generally, the approximate pH of honey is 4.0 (Ouchemoukh et al., 2007Ouchemoukh, S., Louaileche, H., & Schweitzer, P. (2007). Physicochemical characteristics and pollen spectrum of some Algerian honeys. Food Control, 18(1), 52-58. http://dx.doi.org/10.1016/j.foodcont.2005.08.007.
http://dx.doi.org/10.1016/j.foodcont.200...
). Several physicochemical properties show the differences in the chemical composition of honey (Bogdanov et al., 1999Bogdanov, S., Lüllmann, C., & Martin, P. (1999). Honey quality, methods of analysis and international regulatory standards: review of the work of the International Honey Commission. Mitteilungen aus Lebensmitteluntersuchung und Hygiene, 90(1), 108-125.), and its sensorial, chemical, physical, and microbiological characteristics are investigated to determine its quality. The main criteria are moisture content, ash content, electrical conductivity, diastase activity, free acidity, 5- hydroxymethyl furfuraldehyde (HMF) content, and reducing and non-reducing sugars (Gomes et al., 2010Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P., & Estevinho, L. (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology, 48(2), 544-548. http://dx.doi.org/10.1016/j.fct.2009.11.029. PMid:19909782.
http://dx.doi.org/10.1016/j.fct.2009.11....
). Honey production has developed well in Turkey thanks to its geographical and climatic conditions. As an important agricultural activity, the beekeeping has a history of thousands of years in Turkey (Kahraman et al., 2010Kahraman, T., Buyukunal, S. K., Vural, A., & Altunatmaz, S. S. (2010). Physico-chemical properties in honey from different regions of Turkey. Food Chemistry, 123(1), 41-44. http://dx.doi.org/10.1016/j.foodchem.2010.03.123.
http://dx.doi.org/10.1016/j.foodchem.201...
). The Black Sea Region ranks the first in terms of production. In 2016, 1,786,996 tons of honey was produced globally and Turkey ranked the second after China with 105,532 tons (Food and Agriculture Organization, 2020Food and Agriculture Organization – FAO, FAOSTAT Livestock. (2020). Primary production of honey, natural: top 10 producers. Rome: FAO.). Turkey has favorable conditions for honey production like climate, topographical structure, and a wide variety of plant flora (Gül & Pehlivan, 2018Gül, A., & Pehlivan, T. (2018). Antioxidant activities of some monofloral honey types produced across Turkey. Saudi Journal of Biological Sciences, 25(6), 1056-1065. http://dx.doi.org/10.1016/j.sjbs.2018.02.011. PMid:30174502.
http://dx.doi.org/10.1016/j.sjbs.2018.02...
; Sari & Ayyildiz, 2012Sari, E., & Ayyildiz, N. (2012). Biological activities and some physicochemical properties of sunflower honeys collected from the Thrace region of Turkey. Pakistan Journal of Biological Sciences: PJBS, 15(23), 1102-1110. http://dx.doi.org/10.3923/pjbs.2012.1102.1110. PMid:24261112.
http://dx.doi.org/10.3923/pjbs.2012.1102...
; Tezcan et al., 2011Tezcan, F., Kolayli, S., Ulusoy, H. S. E., & Erim, F. B. (2011). Evaluation of organic acid, saccharide composition and antioxidant properties of some authentic Turkish honeys. Journal of Food and Nutrition Research, 50, 33-40.). The appearance, nutritional value, composition, and flavor of honey are affected by its botanical origins and the geographic location of beehives (Gül & Pehlivan, 2018Gül, A., & Pehlivan, T. (2018). Antioxidant activities of some monofloral honey types produced across Turkey. Saudi Journal of Biological Sciences, 25(6), 1056-1065. http://dx.doi.org/10.1016/j.sjbs.2018.02.011. PMid:30174502.
http://dx.doi.org/10.1016/j.sjbs.2018.02...
; Tezcan et al., 2011Tezcan, F., Kolayli, S., Ulusoy, H. S. E., & Erim, F. B. (2011). Evaluation of organic acid, saccharide composition and antioxidant properties of some authentic Turkish honeys. Journal of Food and Nutrition Research, 50, 33-40.).

The composition of honey can vary depending on its origin. For example, the mineral sources primarily originate from its raw materials (nectar and honeydew) and the pollen grains (Madejczyk & Baralkiewicz, 2008Madejczyk, M., & Baralkiewicz, D. (2008). Characterization of Polish rape and honeydew honey according to their mineral contents using ICP-MS and F-AAS/AES. Analytica Chimica Acta, 617(1-2), 11-17. http://dx.doi.org/10.1016/j.aca.2008.01.038. PMid:18486635.
http://dx.doi.org/10.1016/j.aca.2008.01....
; Pohl et al., 2011Pohl, P., Sergiel, I., & Prusisz, B. (2011). Direct analysis of honey for the total content of Zn and its fractionation forms by means of flame atomic absorption spectrometry with solid phase extraction and ultrafiltration approaches. Food Chemistry, 125(4), 1504-1509. http://dx.doi.org/10.1016/j.foodchem.2010.10.077.
http://dx.doi.org/10.1016/j.foodchem.201...
). Carbohydrates (glucose and fructose) account for about 80-95% of the matrix, while the total mineral content in honey should not exceed 1%. The second most important content in honey is water, and its ratio depends on several environmental factors during production such as weather and humidity (Alvarez-Suarez et al., 2010Alvarez-Suarez, J. M., Tulipani, S., Romandini, S., Bertoli, E., & Battino, M. (2010). Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism, 3(1), 15-23. http://dx.doi.org/10.1007/s12349-009-0051-6.
http://dx.doi.org/10.1007/s12349-009-005...
; Bogdanov et al., 2008Bogdanov, S., Jurendic, T., Sieber, R., & Gallmann, P. (2008). Honey for nutrition and health: a review. Journal of the American College of Nutrition, 27(6), 677-689. http://dx.doi.org/10.1080/07315724.2008.10719745. PMid:19155427.
http://dx.doi.org/10.1080/07315724.2008....
). Besides, water content affects the basic physical characteristics of honey, such as viscosity, maturity, and crystallization (Machado De-Melo et al., 2018Machado De-Melo, A. A., Almeida-Muradian, L. B., Sancho, M. T., & Pascual-Maté, A. (2018). Composition and properties of Apis mellifera honey: a review. Journal of Apicultural Research, 57(1), 5-37. http://dx.doi.org/10.1080/00218839.2017.1338444.
http://dx.doi.org/10.1080/00218839.2017....
). Viscosity of ripe honey is higher, with water content of below 22%, leading to non growth of microorganisms and longer shelf life of ripe honey (Aparna & Rajalakshmi, 1999Aparna, A. R., & Rajalakshmi, D. (1999). Honey-its characteristics, sensory aspects, and applications. Food Reviews International, 15(4), 455-471. http://dx.doi.org/10.1080/87559129909541199.
http://dx.doi.org/10.1080/87559129909541...
), and significantly improving the re-ripe honey market acceptance and increasing the beekeepers’ profit given the the production rate (Guo et al., 2019Guo, N., Wang, Q., Shen, L., Wang, L., & Zhao, Y. (2019). An effective and economic method to produce re-ripe honey with honeybee colonies. Food Science and Technology, 39(Suppl. 2), 510-516. http://dx.doi.org/10.1590/fst.23618.
http://dx.doi.org/10.1590/fst.23618...
). It is known that fermentation is another factor that affects the honey composition when moisture exceeds 18%, especially after a long storage time (Bogdanov et al., 2008Bogdanov, S., Jurendic, T., Sieber, R., & Gallmann, P. (2008). Honey for nutrition and health: a review. Journal of the American College of Nutrition, 27(6), 677-689. http://dx.doi.org/10.1080/07315724.2008.10719745. PMid:19155427.
http://dx.doi.org/10.1080/07315724.2008....
). Proteins, minerals, enzymes, vitamins, organic acids, and phenolic compounds are minor components of honey (Pohl et al., 2011Pohl, P., Sergiel, I., & Prusisz, B. (2011). Direct analysis of honey for the total content of Zn and its fractionation forms by means of flame atomic absorption spectrometry with solid phase extraction and ultrafiltration approaches. Food Chemistry, 125(4), 1504-1509. http://dx.doi.org/10.1016/j.foodchem.2010.10.077.
http://dx.doi.org/10.1016/j.foodchem.201...
). Protein, enzyme, and vitamin contents of honey are obtained from the pollen contents and secretions of honeybees (Apis mellifera) (Draiaia et al., 2014Draiaia, R., Rezki, A. R., Khalil, B. n., & Chefrour, E. (2014). Quality of some Algerian honey: study of botanical and some physicochemical parameters. Middle East Journal of Scientific Research, 22(9), 1363-1371.; Krell, 1996Krell, R. (1996). Value-added products from beekeeping (FAO Agricultural Services Bulletin, No. 124). Rome: Food and Agriculture Organization of the United Nations.). Honey includes a few amino acids, and proline is recognized as the major component (Serra Bonvehí & Escolà Jordà, 1997Serra Bonvehí, J., & Escolà Jordà, R. (1997). Nutrient composition and microbiological quality of honeybee-collected pollen in Spain. Journal of Agricultural and Food Chemistry, 45(3), 725-732. http://dx.doi.org/10.1021/jf960265q.
http://dx.doi.org/10.1021/jf960265q...
). The acidity of honey is due to its organic acid contents generating its characteristic flavor (Krell, 1996Krell, R. (1996). Value-added products from beekeeping (FAO Agricultural Services Bulletin, No. 124). Rome: Food and Agriculture Organization of the United Nations.). Hydroxymethylfurfural (HMF) is generally used to evaluate the freshness of honey as its amount is limited in freshly harvested honey. It is mainly produced from sugars degradation, basically from fructose, because it is more labile than sucrose and glucose. The amount of HMF increases during the processes of handling, extraction, and storage (Al-Farsi et al., 2018Al-Farsi, M., Al-Belushi, S., Al-Amri, A., Al-Hadhrami, A., Al-Rusheidi, M., & Al-Alawi, A. (2018). Quality evaluation of Omani honey. Food Chemistry, 262, 162-167. http://dx.doi.org/10.1016/j.foodchem.2018.04.104. PMid:29751904.
http://dx.doi.org/10.1016/j.foodchem.201...
; Visquert et al., 2014Visquert, M., Vargas, M., & Escriche, I. (2014). Effect of postharvest storage conditions on the colour and freshness parameters of raw honey. International Journal of Food Science & Technology, 49(1), 181-187. http://dx.doi.org/10.1111/ijfs.12296.
http://dx.doi.org/10.1111/ijfs.12296...
).

The purpose of this study was to investigate the differences in the physicochemical properties of flower honey (Apis mellifera) produced in the Black Sea Region of Turkey between 2016 and 2019 to see if they changed over the years and if the physicochemical properties met the quality standards for honey.

2 Materials and methods

This research was carried out on the honey samples (Apis mellifera) produced in Kastamonu, a province in Turkey. The samples were collected from 40 producers of honey in Kastamonu, a province located in the Black Sea Region, the northern part of Turkey, between 2016 and 2019. These producers were the members of the Turkish Association of Beekeepers. The samples were collected using 200-gram glass jars every year in August. The samples were stored at room temperature in a dark place until the analyses were carried out, taking approximately one month. We carried out the quality analysis according to the Turkish Food Codex Communiqué on Honey and the EU standards, which included sugar content analysis (Fructose/Glucose ratio, Total Fructose, and Glucose), diastase activity, proline, electrical conductivity, moisture, Hydroxymethylfurfural (HMF), and acidity (Turkey, 2012Turkey, Ministry of Agriculture and Forestry. (2012, July 27). Turkish food codex communiqué on honey-2012/58. Official Journal.).

2.1 Statistical analysis

Statistical analyses were carried out using SPSS (v.23, IBM Corp., Armonk, NY). Descriptive statistics were presented in frequency (n) and percentage (%) for the categorical variables and median with minimum and maximum values for the numeric variables. The Kolmogorov-Smirnov test was used to test the normality of the research variables. The variables with a significance level above 0.05 were accepted to be normally distributed. The t-test, Wilcox, one-way Anova, and Kruskal-Wallis tests were used to examine the physicochemical properties of flower honey in terms of demographics variables.

3 Results and discussion

As can be seen in Table 1, the mean fructose/glucose ratio was found as 1.15(0.24), the mean total fructose and glucose (%) as 64.54 (13.95), the mean diastase (shade) as 14.4 (12.67), the mean proline (mg/kg) as 464.69(218.14), the mean electrical conductivity (ms/cm) as 0.54 (0.38), the mean moisture contents as 17.32(3.91), the mean HMF content (mg/kg) as 18.61(8.14), and the mean acidity content as 24.53(9.89).

Table 1
The mean values of physicochemical parameters.

As can be seen in Table 2, there is a statistically significant difference in moisture content (p-value =.009) in four years while there is no statistically significant difference in the fructose/glucose ratio, electrical conductivity (ms/cm), and the contents of total fructose and glucose (%), diastase (shade), proline (mg/kg), HMF (mg/kg), and acidity (meq/kg).

Table 2
The difference in the physicochemical quality measures of the honey samples in over the course of 4 years (n=40).

To estimate the mean of the samples, a confidence interval on the mean is estimated. Table 3 shows the confidence level of the mean values of the variables. The results showed that the confidence intervals of the variables were as follows: fructose/glucose ratio (0.9-1.4), (1.08, 1.26); total fructose and glucose (%)>60, (65.54, INF); diastase (shade)>8, (11.74, INF); proline (mg/kg)>300, (406.51, INF); electrical conductivity (ms/cm)<0.8, (-INF, 0.55); moisture (%)<20, (-INF, 18.29); HMF (mg/kg)<40, (-INF, 21.42); and acidity (meq/kg)<50, (-INF, 27.17).

Table 3
The confidence level of the mean values of the variables.

Table 3 shows that the samples met the standards for total fructose and glucose, diastase (shade), proline, electrical conductivity, moisture, HMF (mg/kg), and acidity; but not for fructose/glucose ratio. In the current study, we investigated 40 honey samples collected from the Black Sea Region of Turkey. The findings showed that fructose/glucose ratio, total fructose and glucose (%), diastase (shade), proline (mg/kg), electrical conductivity (ms/cm), HMF (mg/kg), and acidity (meq/kg) did not change in four years while the moisture content was the only variable which significantly differed in four years. The quality of the studied samples remained stable over the course of four years of study. The honey samples met the quality standards for total fructose and glucose, diastase (shade), proline, electrical conductivity, moisture, HMF (mg/kg), and acidity but not for fructose/glucose ratio. Several studies revealed that differences in climate, processing, storage conditions, and maturity affect the quality and biochemical properties of honey (Silva et al., 2016Silva, P. M., Gauche, C., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Honey: chemical composition, stability and authenticity. Food Chemistry, 196, 309-323. http://dx.doi.org/10.1016/j.foodchem.2015.09.051. PMid:26593496.
http://dx.doi.org/10.1016/j.foodchem.201...
). International Honey Commission proposed some quality criteria for honey, including several parameters such as moisture content, sugar content, mineral containment, acidity, electrical conductivity, and HMF. The quality and authenticity of honey are crucial for preserving the food safety and generating favorable effects on the health of consumers (Bogdanov, 2009Bogdanov, S. (2009). Harmonised methods of the International Honey Commission (pp. 1-63). İstanbul: IHC.). Honey production is regulated by the Turkish Food Codex Communiqué on Honey in Turkey, one of the world's largest honey producers. The provisions of this regulation are similar to those in the international regulations, and it has been updated according to the latest developments announced by the international authority. Several specific laboratories in Turkey have updated their standards for evaluating honey according to international and Turkish Food Codex Communiqué on Honey.

Honey is a natural and healthy product with a changing commercial value depending on its botanic and geographic origins (Trifković et al., 2017Trifković, J., Andrić, F., Ristivojević, P., Guzelmeric, E., & Yesilada, E. (2017). Analytical methods in tracing honey authenticity. Journal of AOAC International, 100(4), 827-839. http://dx.doi.org/10.5740/jaoacint.17-0142. PMid:28527183.
http://dx.doi.org/10.5740/jaoacint.17-01...
). The stability of honey against fermentation and granulation can be determined by measuring the amount of water in honey (Dyce, 1975Dyce, E. J. (1975). Producing finely granulated or creamed honey. In E. Crane (Ed.), Honey: a comprehensive survey (pp. 293-306). London: Heinemann.). Saxena et al. (2010)Saxena, S., Gautam, S., & Sharma, A. (2010). Physical, biochemical and antioxidant properties of some Indian honeys. Food Chemistry, 118(2), 391-397. http://dx.doi.org/10.1016/j.foodchem.2009.05.001.
http://dx.doi.org/10.1016/j.foodchem.200...
showed that the moisture content of honey, which is one of the descriptive quality parameters, ranged from 10.5% to 18.2% for fresh honey (Saxena et al., 2010Saxena, S., Gautam, S., & Sharma, A. (2010). Physical, biochemical and antioxidant properties of some Indian honeys. Food Chemistry, 118(2), 391-397. http://dx.doi.org/10.1016/j.foodchem.2009.05.001.
http://dx.doi.org/10.1016/j.foodchem.200...
). The acceptable limit for moisture content is 20% (Kędzierska-Matysek et al., 2016Kędzierska-Matysek, M., Florek, M., Wolanciuk, A., & Skałecki, P. (2016). Effect of freezing and room temperatures storage for 18 months on quality of raw rapeseed honey (Brassica napus). Journal of Food Science and Technology, 53(8), 3349-3355. http://dx.doi.org/10.1007/s13197-016-2313-x. PMid:27784929.
http://dx.doi.org/10.1007/s13197-016-231...
). In our study, all the honey samples complied with the commonly accepted levels for moisture content. The moisture content of honey varies depending on the degree of maturity reached in the hive, the moisture content of the original plant, and the harvest season (Finola et al., 2007Finola, M. S., Lasagno, M. C., & Marioli, J. M. (2007). Microbiological and chemical characterization of honeys from central Argentina. Food Chemistry, 100(4), 1649-1653. http://dx.doi.org/10.1016/j.foodchem.2005.12.046.
http://dx.doi.org/10.1016/j.foodchem.200...
). Besides, the variations in moisture content can be due to the floral origins and composition of honey (Malika et al., 2005Malika, N., Mohamed, F., & Chakib, E. A. (2005). Microbiological and physicochemical properties of Moroccan honey. International Journal of Agriculture and Biology, 7(5), 773-776.), and moisture content can vary from year to year, which is consistent with the result reached in our study that showed a variation in moisture content in 4 years. Derebaşı et al. (2014)Derebaşı, E., Bulut, G., Col, M., Güney, F., Yaşar, N., & Ertürk, Ö. (2014). Physicochemical and residue analysis of honey from Black Sea region of Turkey. Fresenius Environmental Bulletin, 23(1), 10-17. found that the electrical conductivity (mS/cm) of honey samples varied from 0.12 to 2.42 with a mean value of 0.48 ± 0.03 mS/cm, very similar to the mean electrical conductivity (0.54 mS/cm) found in our study. The mean electrical conductivity (mS/cm) of the honey samples in the current study was below the maximum limit determined by TFC and EU standards (0.8 mS/cm).

Honey contains a small proportion of organic acids, which occur due to aerobic and anaerobic fermentation and give a characteristic taste to honey (Tezcan et al., 2011Tezcan, F., Kolayli, S., Ulusoy, H. S. E., & Erim, F. B. (2011). Evaluation of organic acid, saccharide composition and antioxidant properties of some authentic Turkish honeys. Journal of Food and Nutrition Research, 50, 33-40.).

In this study, the free acidity of the honey samples varied between 10.2 meq/kg and 45.6 meq/kg with a mean value of 24.53 meq acid/kg. The study samples did not exceed the limit determined by TFC and EU (<50 meq acid/kg), indicating that all the honey samples were fresh. Our study results are similar to those found in the studies by Gül (2008)Gül, A. (2008). Türkiye’de üretilen bazı balların yapısal özelliklerinin gıda güvenliği bakımından araştırılması (Doktora tezi). Fen Bilimleri Enstitüsü, Hatay. and Yılmaz & Küfrevioğlu (2001). The mean value obtained in our study was higher than that found by Russo Almeida (1997)Russo Almeida, P. A. (1997). Honey of transmontane Terra Quente: some chemical parameters of honey from transmontane Terra Quente. Apicultor, 5, 29-35. (16.50 meq acid/kg) but lower than the mean value of 31.62 meq acid/kg reported by Sunay (2006)Sunay, A. E. (2006). Problem of antibiotic residues in honey. Uludag Bee Journal, 4, 143-148.. Al-Khalifa & Al-Arify (1999)Al-Khalifa, A. S., & Al-Arify, I. A. (1999). Physicochemical characteristics and pollen spectrum of some Saudi honeys. Food Chemistry, 67(1), 21-25. http://dx.doi.org/10.1016/S0308-8146(99)00096-5.
http://dx.doi.org/10.1016/S0308-8146(99)...
, Azeredo et al. (2003)Azeredo, L. C., Azeredo, M. A. A., Souza, S. R., & Dutra, V. M. L. (2003). Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80(2), 249-254. http://dx.doi.org/10.1016/S0308-8146(02)00261-3.
http://dx.doi.org/10.1016/S0308-8146(02)...
, and Sari & Ayyildiz (2012)Sari, E., & Ayyildiz, N. (2012). Biological activities and some physicochemical properties of sunflower honeys collected from the Thrace region of Turkey. Pakistan Journal of Biological Sciences: PJBS, 15(23), 1102-1110. http://dx.doi.org/10.3923/pjbs.2012.1102.1110. PMid:24261112.
http://dx.doi.org/10.3923/pjbs.2012.1102...
reported that the total acidity of the samples varied between 32.3 meq/kg and 72.2 meq/kg, 2.8 meq/kg and 39.5 meq/kg, and 16.0 meq/kg and 39.5 meq/kg, respectively (Al-Khalifa & Al-Arify, 1999Al-Khalifa, A. S., & Al-Arify, I. A. (1999). Physicochemical characteristics and pollen spectrum of some Saudi honeys. Food Chemistry, 67(1), 21-25. http://dx.doi.org/10.1016/S0308-8146(99)00096-5.
http://dx.doi.org/10.1016/S0308-8146(99)...
; Azeredo et al., 2003Azeredo, L. C., Azeredo, M. A. A., Souza, S. R., & Dutra, V. M. L. (2003). Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80(2), 249-254. http://dx.doi.org/10.1016/S0308-8146(02)00261-3.
http://dx.doi.org/10.1016/S0308-8146(02)...
; Sari & Ayyildiz, 2012Sari, E., & Ayyildiz, N. (2012). Biological activities and some physicochemical properties of sunflower honeys collected from the Thrace region of Turkey. Pakistan Journal of Biological Sciences: PJBS, 15(23), 1102-1110. http://dx.doi.org/10.3923/pjbs.2012.1102.1110. PMid:24261112.
http://dx.doi.org/10.3923/pjbs.2012.1102...
).

The acidity which is a remarkable characteristic of the flavor, associated with the maturation state of honey (Braga et al., 2020Braga, D. C., Liberato, M. D. C. T. C., Lima, V. L. F., & Araújo, J. A. M. No. (2020). Analytical study of the physicochemical characteristics from Melipona subnitida D. honey in adequation to Brazilian law. Food Science and Technology, 40(Suppl. 1), 217-221. http://dx.doi.org/10.1590/fst.08919.
http://dx.doi.org/10.1590/fst.08919...
) did not change in our study over the course of 4 years. The variation of acidity among different samples which was reported in the literature may be due to the variations in floral type of plants (Küçük et al., 2007Küçük, M., Kolaylı, S., Karaoğlu, Ş., Ulusoy, E., Baltacı, C., & Candan, F. (2007). Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry, 100(2), 526-534. http://dx.doi.org/10.1016/j.foodchem.2005.10.010.
http://dx.doi.org/10.1016/j.foodchem.200...
) and harvest season (Singh & Bath, 1997Singh, N., & Bath, P. K. (1997). Quality evaluation of different types of Indian honey. Food Chemistry, 58(1), 129-133. http://dx.doi.org/10.1016/S0308-8146(96)00231-2.
http://dx.doi.org/10.1016/S0308-8146(96)...
). Honey is a natural product and contains a highly concentrated, complex solution of sugar. However, adulteration of honey with cheap sugar syrup is a common problem, mainly by the addition of sucrose. The addition of fructose or glucose may alter the fructose/glucose ratio (Gül & Pehlivan, 2018Gül, A., & Pehlivan, T. (2018). Antioxidant activities of some monofloral honey types produced across Turkey. Saudi Journal of Biological Sciences, 25(6), 1056-1065. http://dx.doi.org/10.1016/j.sjbs.2018.02.011. PMid:30174502.
http://dx.doi.org/10.1016/j.sjbs.2018.02...
; Trifković et al., 2017Trifković, J., Andrić, F., Ristivojević, P., Guzelmeric, E., & Yesilada, E. (2017). Analytical methods in tracing honey authenticity. Journal of AOAC International, 100(4), 827-839. http://dx.doi.org/10.5740/jaoacint.17-0142. PMid:28527183.
http://dx.doi.org/10.5740/jaoacint.17-01...
). In this study, the mean glucose and fructose contents of the honey samples varied between 0.73 and 79.00%, which exceeded the minimum required level of 60% according to the international quality regulations. It was reported in the literature that glucose and fructose contents of honey samples varied between 49.7% and 73.5% (Al-Khalifa & Al-Arify, 1999Al-Khalifa, A. S., & Al-Arify, I. A. (1999). Physicochemical characteristics and pollen spectrum of some Saudi honeys. Food Chemistry, 67(1), 21-25. http://dx.doi.org/10.1016/S0308-8146(99)00096-5.
http://dx.doi.org/10.1016/S0308-8146(99)...
; Azeredo et al., 2003Azeredo, L. C., Azeredo, M. A. A., Souza, S. R., & Dutra, V. M. L. (2003). Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80(2), 249-254. http://dx.doi.org/10.1016/S0308-8146(02)00261-3.
http://dx.doi.org/10.1016/S0308-8146(02)...
; Ozcan & Olmez, 2014Ozcan, M. M., & Olmez, C. (2014). Some qualitative properties of different monofloral honeys. Food Chemistry, 163, 212-218. http://dx.doi.org/10.1016/j.foodchem.2014.04.072. PMid:24912718.
http://dx.doi.org/10.1016/j.foodchem.201...
), which is consistent with our study results, showing that the samples met the standards for total fructose and glucose.

On the other hand, the fructose/glucose ratio, which indicates the crystallization of honey, was found to be between 0.60 and 1.67 with a mean value of 1.15, which showed that the samples did not tend to crystallize faster and were less viscous (F/G > 1.0). So, the honey samples did not meet the quality standard for the fructose/glucose ratio.

It is known that the composition of honey depends on not only the diversity of flowers but also some other factors such as environmental factors, harvesting climate, processing, and storage technology (Kaškonienė & Venskutonis, 2010Kaškonienė, V., & Venskutonis, P. R. (2010). Floral markers in honey of various botanical and geographic origins: a review. Comprehensive Reviews in Food Science and Food Safety, 9(6), 620-634. http://dx.doi.org/10.1111/j.1541-4337.2010.00130.x.
http://dx.doi.org/10.1111/j.1541-4337.20...
; Ozcan‐Sinir et al., 2020Ozcan‐Sinir, G., Copur, O. U., & Barringer, S. A. (2020). Botanical and geographical origin of Turkish honeys by selected‐ion flow‐tube mass spectrometry and chemometrics. Journal of the Science of Food and Agriculture, 100(5), 2198-2207. http://dx.doi.org/10.1002/jsfa.10244. PMid:31901138.
http://dx.doi.org/10.1002/jsfa.10244...
).

Diastase activity varies depending on several factors such as exposure to high temperatures and long storage periods, which inactivate diastase (Ünal & Küplülü, 2006Ünal, C., & Küplülü, Ö. (2006). Chemical quality of strained honey consumed in Ankara. Veterinary Journal of Ankara University, 53, 1-4.). In our study, there was no change in the diastase activity in four years.

Different diastase values were reported from different countries in the literature, from 10.9 to 13.9 in Brazil and from 3.3 to 13.9 in Saudi Arabia (Al-Khalifa & Al-Arify, 1999Al-Khalifa, A. S., & Al-Arify, I. A. (1999). Physicochemical characteristics and pollen spectrum of some Saudi honeys. Food Chemistry, 67(1), 21-25. http://dx.doi.org/10.1016/S0308-8146(99)00096-5.
http://dx.doi.org/10.1016/S0308-8146(99)...
; Azeredo et al., 2003Azeredo, L. C., Azeredo, M. A. A., Souza, S. R., & Dutra, V. M. L. (2003). Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80(2), 249-254. http://dx.doi.org/10.1016/S0308-8146(02)00261-3.
http://dx.doi.org/10.1016/S0308-8146(02)...
). Sahinler et al. (2001)Sahinler, N., Sahinler, S., & Gül, A. (2001). Hatay yöresi ballarının bilesimi ve biyokimyasal analizi. M.K.Ü Ziraat Fakultesi Dergisi, 6, 93-108. and Sari & Ayyildiz (2012)Sari, E., & Ayyildiz, N. (2012). Biological activities and some physicochemical properties of sunflower honeys collected from the Thrace region of Turkey. Pakistan Journal of Biological Sciences: PJBS, 15(23), 1102-1110. http://dx.doi.org/10.3923/pjbs.2012.1102.1110. PMid:24261112.
http://dx.doi.org/10.3923/pjbs.2012.1102...
reported that the diastase value of the honey samples collected from Turkey varied within the range of 1-23 and 17.9-38.5, respectively (Sahinler et al., 2001Sahinler, N., Sahinler, S., & Gül, A. (2001). Hatay yöresi ballarının bilesimi ve biyokimyasal analizi. M.K.Ü Ziraat Fakultesi Dergisi, 6, 93-108.; Sari & Ayyildiz, 2012Sari, E., & Ayyildiz, N. (2012). Biological activities and some physicochemical properties of sunflower honeys collected from the Thrace region of Turkey. Pakistan Journal of Biological Sciences: PJBS, 15(23), 1102-1110. http://dx.doi.org/10.3923/pjbs.2012.1102.1110. PMid:24261112.
http://dx.doi.org/10.3923/pjbs.2012.1102...
). Diastase value should not be lower than 8 according to the legal regulation. In our study, the mean diastase values of the honey samples was found to be 14.4, which means that the samples in our study met the quality standards. We assumed that high diastase values in our study show that honey samples were not exposed to high temperature.

Previous studies reported change of protein values of honey samples between 0.33% and 1.2% (Azeredo et al., 2003Azeredo, L. C., Azeredo, M. A. A., Souza, S. R., & Dutra, V. M. L. (2003). Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80(2), 249-254. http://dx.doi.org/10.1016/S0308-8146(02)00261-3.
http://dx.doi.org/10.1016/S0308-8146(02)...
; Ozcan‐Sinir et al., 2020Ozcan‐Sinir, G., Copur, O. U., & Barringer, S. A. (2020). Botanical and geographical origin of Turkish honeys by selected‐ion flow‐tube mass spectrometry and chemometrics. Journal of the Science of Food and Agriculture, 100(5), 2198-2207. http://dx.doi.org/10.1002/jsfa.10244. PMid:31901138.
http://dx.doi.org/10.1002/jsfa.10244...
; Ozcan & Olmez, 2014Ozcan, M. M., & Olmez, C. (2014). Some qualitative properties of different monofloral honeys. Food Chemistry, 163, 212-218. http://dx.doi.org/10.1016/j.foodchem.2014.04.072. PMid:24912718.
http://dx.doi.org/10.1016/j.foodchem.201...
). Honey contains low amounts of amino acids, and proline is a major component (Serra Bonvehí & Escolà Jordà, 1997Serra Bonvehí, J., & Escolà Jordà, R. (1997). Nutrient composition and microbiological quality of honeybee-collected pollen in Spain. Journal of Agricultural and Food Chemistry, 45(3), 725-732. http://dx.doi.org/10.1021/jf960265q.
http://dx.doi.org/10.1021/jf960265q...
). The mean proline content of the honey samples collected from the Black Sea region of Turkey was 464.69 mg/kg, which met the standard for proline content i.e. above 300 mg/kg.

The HMF mean value of the honey samples was found to be 18.61 mg/kg. Azeredo et al. (2003)Azeredo, L. C., Azeredo, M. A. A., Souza, S. R., & Dutra, V. M. L. (2003). Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80(2), 249-254. http://dx.doi.org/10.1016/S0308-8146(02)00261-3.
http://dx.doi.org/10.1016/S0308-8146(02)...
reported that HMF values of the honey samples varied between 3.24 mg/kg and 4.12 mg/kg. The internationally acknowledged regulations state that HMF content should not exceed 40 mg/kg (Aloisi, 2010Aloisi, P. V. (2010). Determination of quality chemical parameters of honey from Chubut (Argentinean Patagonia). Chilean Journal of Agricultural Research, 70(4), 640-645. http://dx.doi.org/10.4067/S0718-58392010000400015.
http://dx.doi.org/10.4067/S0718-58392010...
), and all the honey samples in our study complied with this level. Contrary to our study results, Ünal & Küplülü (2006)Ünal, C., & Küplülü, Ö. (2006). Chemical quality of strained honey consumed in Ankara. Veterinary Journal of Ankara University, 53, 1-4. reported a HMF value of 74.51 mg/kg for the flower honey produced in Ankara, considerably higher than the international standard limit.

Enzymatic inactivation occurs during a long time heating process, and HMF is formed by fructose degradation. Moreover, exposure to high temperature for a long time can cause nutritional loss in honey (Ozcan & Olmez, 2014Ozcan, M. M., & Olmez, C. (2014). Some qualitative properties of different monofloral honeys. Food Chemistry, 163, 212-218. http://dx.doi.org/10.1016/j.foodchem.2014.04.072. PMid:24912718.
http://dx.doi.org/10.1016/j.foodchem.201...
). Our results showed that the honey samples collected in all years were not exposed to high temperature. HMF content is regarded as a parameter determining the freshness of honey samples because it is not found in fresh honey and increases during processing of the product (Derebaşı et al., 2014Derebaşı, E., Bulut, G., Col, M., Güney, F., Yaşar, N., & Ertürk, Ö. (2014). Physicochemical and residue analysis of honey from Black Sea region of Turkey. Fresenius Environmental Bulletin, 23(1), 10-17.). In our study, no variation was observed in the HMF content with passage of time. Braga et al. (2020)Braga, D. C., Liberato, M. D. C. T. C., Lima, V. L. F., & Araújo, J. A. M. No. (2020). Analytical study of the physicochemical characteristics from Melipona subnitida D. honey in adequation to Brazilian law. Food Science and Technology, 40(Suppl. 1), 217-221. http://dx.doi.org/10.1590/fst.08919.
http://dx.doi.org/10.1590/fst.08919...
found higher divergence of some patterns such as HMF, moisture, and acidity as compared to the the legislation standards.

International honey standards are given in the European Honey Directive and in the Codex Alimentarius Standard for Honey (Bogdanov et al., 1999Bogdanov, S., Lüllmann, C., & Martin, P. (1999). Honey quality, methods of analysis and international regulatory standards: review of the work of the International Honey Commission. Mitteilungen aus Lebensmitteluntersuchung und Hygiene, 90(1), 108-125.). The honey quality standards in the draft CL 1998/12-S of the Codex Alimentarius and the EU Draft 96/0114 (CNS) are as follows: moisture content ≤ 21 g/100 g, reducing sugars content ≥ 65 g/100 g, sucrose content ≤5 g/100 g, acidity ≤ 40 meq/kg, diastase activity ≥ 8, hydroxymethylfurfural content ≤ 40 mg/kg, and electrical conductivity ≤ 0.8 mS/cm; and honey control laboratories recommend a minimum proline content of 180 mg/kg (Bogdanov et al., 1999Bogdanov, S., Lüllmann, C., & Martin, P. (1999). Honey quality, methods of analysis and international regulatory standards: review of the work of the International Honey Commission. Mitteilungen aus Lebensmitteluntersuchung und Hygiene, 90(1), 108-125.), which are somewhat in line with the values in the Turkish Food Codex Communiqué on Honey.

4 Conclusion

It was concluded that only moisture content changed significantly in four years. The quality of the samples remained stable in four years. The quality standards for honey, such as total fructose and glucose, diastase (shade), proline, electrical conductivity, moisture, HMF (mg/kg), and acidity were met; but the standard for fructose/glucose ratio was not met in the samples. Most of the honey samples obtained from the Black Sea Region of Turkey were compatible with the standards stipulated in the Turkish Food Codex Communiqué on Honey and the Codex Alimentarius Standard for Honey. The authenticity and commercial value of honey should be evaluated based on the internationally accepted regulations to protect both the consumer and the market.

  • Practical Application: This study gives useful insights into whether the physicochemical properties and quality of flower honey differ depending on the year of production.

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Publication Dates

  • Publication in this collection
    28 Apr 2021
  • Date of issue
    2022

History

  • Received
    21 Nov 2020
  • Accepted
    18 Jan 2021
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