Effects of medications and laser on induced tooth movement and associated root resorption: Four key points

Contact address: Alberto Consolaro E-mail: consolaro@uol.com.br Root resorption during orthodontic treatment is a frequent phenomenon, but should not be considered normal or physiological. In numerous clinical cases of orthodontic treatment, apical root resorption is practically inevitable, although predictable when predictive factors are carefully defined.2 Although predictive factors are used for analysis, treatment planning often indicates a very high probability of root resorption, which, in such cases, is inevitable and, as long as predicted and explained to the patient, should not be classified as iatrogenic, as it is the biological cost to pay for the esthetic and functional benefits of orthodontic treatment. Studies have sought ways to speed up tooth movement and reduce orthodontic treatment time. Several factors have been investigated to find ways to reduce the frequency and severity of root resorption during orthodontic treatment. In this search, mediEffects of medications and laser on induced tooth movement and associated root resorption: Four key points orthodontic insight

Root resorption during orthodontic treatment is a frequent phenomenon, but should not be considered normal or physiological.In numerous clinical cases of orthodontic treatment, apical root resorption is practically inevitable, although predictable when predictive factors are carefully deined. 2 Although predictive factors are used for analysis, treatment planning oten indicates a very high probability of root resorption, which, in such cases, is in-evitable and, as long as predicted and explained to the patient, should not be classiied as iatrogenic, as it is the biological cost to pay for the esthetic and functional beneits of orthodontic treatment.
Studies have sought ways to speed up tooth movement and reduce orthodontic treatment time.Several factors have been investigated to find ways to reduce the frequency and severity of root resorption during orthodontic treatment.In this search, medi-

Efects of medications and laser on induced tooth movement and associated root resorption: Four key points orthodontic insight
The following four fundamental points on the use of experimental models will be described to ensure an accurate evaluation of the efects of medication and laser therapy on induced tooth movement and associated root resorption: (1) If the objective is to check the efect on root resorption, the forces experimentally applied must produce a lesion on the cementoblast layer in all specimens; (2) If the objective is to optimize induced tooth movement and reduce treatment time without side efects, the forces experimentally applied should not produce a lesion in the cementoblast layer in any specimen; (3) The laser therapy operator, the person administering medication and the person that places appliances should not know which animals will efectively receive the test treatment, and the control groups should receive placebo treatments; (4) CT and microscopic analysis of the specimens should be random, and the group to which the specimen belongs should not be identiied to ensure that the person reading images and the pathologists are not inluenced in their evaluation of phenomena.These measures will ensure that results are more reliable and easier to extrapolate to orthodontic clinical practice.cations and other tools to achieve those objectives are investigated, such as the use of phototherapy, particularly laser and its variables.
The experimental model of induced tooth movement developed by Henler and Nanda 13 is the one most frequently used worldwide to evaluate the efect of several factors associated with orthodontic clinical practice, and was revised by Ren et al. 21in 2004.In 2009, Fracalossi et al 6 suggested a standardization of cross-sectional cuts of murine molars for the analysis of phenomena associated with induced tooth movement, and Santamaria Jr 22 described a practical way to quantify it.Still in 2009, Maldonado 19 investigated the efect of medications, particularly analgesic and anti-inlammatory drugs, on induced tooth movement and the frequency and severity of associated induced tooth movement.
The use of phototherapy, particularly laser light therapy, in induced tooth movement has raised the interest of clinicians and researchers.In 2013, Vasconcelos, 25 in his doctorate thesis, found that the use of lower level laser did not affect root resorption induced by orthodontic treatment.

THE FOUR FUNDAMENTAL POINTS IN THE CONCEPTION AND DESIGN OF AN EXPERIMENTAL MODEL
Several experimental studies about this topic have not described whether they followed these four fundamental points during study design, planning, method choice and discussion of results: 1. Cementoblast death.During tooth movement, the root is protected against inlammatory tooth resorption by cementoblasts, which do not have receptors for mediators that interfere with bone remodeling.
If the objective of a study is to investigate the effect of medications or phototherapy on the frequency and severity of root resorption, the forces of the experimental model should efectively and conirmedly produce lesions on the cementoblast layer, thus eliminating this root protection.The lesion on this layer, and the consequent death of cementoblasts, is the key phenomenon to initiate inlammatory root resorption.
If the experimental model, randomly and in an uncontrolled way, does not ensure that cementoblasts will die in all animals, results about the protective efect of a medication or phototherapy may be compromised.Teeth with preserved cementoblasts does not undergo inlammatory tooth resorption with or without the application of medication or phototherapy.

The preservation of cementoblasts. During desired, ideal and normal induced tooth movement, cementoblasts should be preserved.
Cell stress and inlammation of the periodontal ligament should not afect cementoblasts during orthodontic movement.The forces applied should not result in the death of cementoblasts when the objective is to test the optimization of tooth movement by means of medication or phototherapy.
Medications and phototherapy does not increase or decrease cementoblast resistance to anoxia induced by the compression of periodontal blood vessels during orthodontic movement.When there is direct and excessive compression of periodontal structures, or even when the tooth being moved applies force to the alveolar bone, medications and phototherapy does not avoid the death of cementoblasts.
When medications and phototherapy are tested to optimize tooth movement and shorten treatment time, the experimental model, as well as the intensity and concentration of forces, should efectively preserve the Efects of medications and laser on induced tooth movement and associated root resorption: Four key points integrity of the cementoblasts layer.Excessive force induces hyalinization of the periodontal ligament and root resorption, which are not part of the desired optimization, that is, tooth movement without collateral damage to periodontal and root tissues.

3.
Applications should be blinded.The phototherapy (laser, for example) operator, as well as the operator that places appliances for tooth movement, should not know which animals will efectively receive the test treatment.Applications should be performed using identical devices; for example, a unit that applies light and the other that does not, but that is a perfect imitation of a laser unit.
Likewise, the person that administer medications, as well as the operator that places the appliances for tooth movement, should not know whether they are administering or not the test drug or a placebo to the animals.The control group should equally receive a mock liquid medication or tablet so that the persons that administer the medication and feed the animals do not know which animals are in the experimental group.
Only at the end of the experiments should the specimens be identiied, grouped and separated according to experimental conditions to which they were submitted, so that there is no other inluence than the experimental variable under evaluation.

Examiners should not know what animal received or not the test.
As the specimens are prepared for CT or microscopic analysis, the pathologist or radiologist should not know which specimens are in the control or experimental group.Random examination of each specimen, not separated into groups, should be conducted according to previously established criteria.Examiners should be free of any type of inluence when they start examining each specimen separately.
Specimens should be identiied, grouped, separated and compared only ater the results are recorded.Therefore, only the variable under test will justify or not the diferences found.

FINAL CONSIDERATIONS
The use of medications and phototherapy in orthodontic movement should be evaluated carefully and free of interests of any nature, particularly commercial interests.To ensure that the environment is rigorously controlled and free of inluences, such as those due to human subjectivity, we described four fundamental points that should characterize any study that uses experimental models of induced tooth movement and associated root resorption: 1. Cementoblast death.If the objective is to check the efect on root resorption, the forces experimentally applied must produce a lesion on the cementoblast layer of all specimens.

The preservation of cementoblasts.
If the objective is to optimize induced tooth movement and reduce treatment time without side efects, the forces experimentally applied should not injure the cementoblast layer of any specimen.

3.
Applications should be blinded.The person applying phototherapy or administering medication and the operator that places appliances should not know which animals will efectively receive the test treatment, and specimens in the control group should receive mock treatments.

Evaluators should not know what animal received or not the test.
CT and microscopic analysis of the specimens should be random, and the group to which the specimen belongs should not be identiied, to ensure that radiologists and pathologists are not afected in their evaluation of phenomena.
These measures will ensure that results are free of subjective inluences from investigators and, at the same time, are more reliable and easier to extrapolate to clinical orthodontic practice.