The present work consists of the development and validation of HPLC analytical methodology for the evaluation of 7-hydroxy-4',6-dimethoxyisoflavone in cells suspension and callus culture of Dipteryx odorata. The validation parameters: analytical curve, linearity, precision, recovery, detection limit and quantification limit were determined and they demonstrated that the analytical procedures for the detection and quantification for this isoflavone follow the requeriments of regulatory RE899/03- National Health Surveillance Agency (ANVISA) and could be applied to cells culture quality control of this vegetal species.
CLAE/DAD; Dipteryx odorata; isoflavona
