The pathogenic, physiological and morphological characterization of eight Pseudocercospora musae isolates were studied. To determine pathogenicity, the isolates were inoculated on banana (Musa spp.) leaves, using two methods. The most efficience in the reproduction of the symptoms was observed in the wound method where all isolates showed pathogenicity, while without wounds, only P-2 and P-4 isolates presented the ability to enter and colonize the host tissue. For the physiological characterization, the behavior of the eight isolates was studied in six culture media and three pH levels, during seven days of incubation at 25 °C, and alternated light. Coconut, potato dextrose agar, and oat media induced higher mycelial growth, while potato dextrose agar, MPA, V-8 juice and Czapek promoted higher production of conidia. The isolates of P. musae grew well at pH 4,5, and the sporulation was favoured at pH 6,5. The cultural characteristics of the isolates showed little variation in topography, coloration, and pigmentation of the colony, according to the medium and pH level used. The morphological characterization showed little, but not significant, variation in size and conidial length/width ratio.
