<i>In Vitro</i> Rooting and Multiplication of <i>Myrcianthes pungens</i> (O. Berg) D. Legrand

Souza, Luana dos Santos de; Campos, Samanta Siqueira de; Avrella, Eduarda Demari; Fior, Claudimar Sidnei; Schwarz, Sérgio Francisco

doi:10.1590/2179-8087.111917

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ORIGINAL ARTICLE, Wood Science and Technology • Floresta Ambient. 27 (1) • 2020 • https://doi.org/10.1590/2179-8087.111917 copy

In Vitro Rooting and Multiplication of Myrcianthes pungens (O. Berg) D. Legrand

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The propagation of guabijuzeiro (M. pungens) by seed is originating plants with high genetic variability and a long juvenile period. Therefore, the objective was to study the multiplication and rooting in vitro of guabijuzeiro in order to get information to enable the clonal propagation. In the multiplication, apical and nodal segments were used, taken from in vitro plantlets, combined with 6-Benzylaminopurine (BAP), naphthaleneacetic acid (NAA) and gibberellic acid (GA). In the rooting, NAA and IBA (indolebutyric acid) were used. The results showed that in the multiplication there was superiority in media with BAP, while rooting was not satisfactory in media with NAA or IBA. Thus, it is possible to multiply M. pungens in vitro using BAP phytoregulator in the multiplication stage. However, the rooting stage needs more tests to induce the production of roots and facilitate acclimatization of the plants obtained in vitro.

Keywords:
environmental economics; forest genetics and improvement; wood preservation

Treatments		Shoots (%)	No. leaves	Height (cm)	No. shoots/expl.
Apical	Control	18 c	2.33 c	0.23 c	0.5 c
	BAP	73 a	7.35 a	0.55 a	1.2 a
	BAP + NAA	57 b	5.58 b	0.46 b	0.8 b
Nodal	Control	12 c	2.17 c	0.13 c	0.3 c
	BAP	80 a	11.63 a	0.82 a	1.6 a
	BAP + NAA	58 b	5.45 b	0.44 b	0.9 b
p value	Explant	0.914	0.206	0.497	0.974
	Phytoregulator	< 0.001	< 0.001	< 0.001	< 0.001
	Explant × Phytoregulator	0.597	0.070	0.050	0.177
CV (%)		9.13	13.6	4.88	29.3

Treatments		Survival (%)	Oxidation (%)	No. leaves
Apical	BAP + ANA	67 b	37 b	6.8 b
Apical	BAP	90 a	9 a	17.6 a
Nodal	BAP + ANA	51 b	33 b	6.5 b
Nodal	BAP	89 a	9 a	18.3 b
p value	Phytoregulator	< 0.001	0.002	< 0.001
	Explant	0.269	0.965	0.895
	Explant × Phytoregulator	0.312	0.563	0.716
CV (%)		25.8	20.6	28.9

Treatments		No. shoots/expl.	No. leaves	Height (cm)
Apical	BAP	3.00 a	23.9 a	0.75 Ba
Apical	3 FIT	1.65 b	10.4 b	0.56 Ba
Nodal	BAP	3.60 a	19.7 a	1.00 Aa
Nodal	3 FIT	1.90 b	13.3 b	0.94 Aa
p value	Phytoregulator	< 0.001	< 0.001	0.429
	Explant	0.509	0.727	0.016
	Explant × Phytoregulator	0.621	0.042	0.529
CV (%)		27.9	26.2	20.4

Treatments (mg L^-1)		Survival (%)	Callus (%)
IBA	0.2	100.00 Aa	16.58 Ba
	0.4	91.58 Aab	16.50 Ba
	0.6	77.67 Bb	11.00 Ba
NAA	0.2	91.67 Aa	44.08 Ab
	0.4	100.00 Aa	86.00 Aa
	0.6	100.00 Aa	88.83 Aa
p value	Phytoregulator	0.109	0.00
	Concentration	0.369	0.012
	Phytoregulator × Concentration	0.027	0.003

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E-mail: floram@ufrrj.br

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[1] CORRESPONDENCE TOEduarda Demari Avrella Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 7.712, CEP 91540-000, Porto Alegre, RS, Brasil e-mail: dudademari@hotmail.com