EFFECT OF THE GENOTYPE ON THE POTENTIAL FOR THE PRODUCTION OF SHOOTS AND ADVENTITIOUS ROOTS IN <i>Eucalyptus grandis</i> Hill ex Maiden <i>IN VITRO</i>

Sobrosa, Rita de Cassia; Corder, Maisa Pimentel Martins

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Artigo Original • Floresta Ambient. 10 (1) • Jan-Mar 2003 •

EFFECT OF THE GENOTYPE ON THE POTENTIAL FOR THE PRODUCTION OF SHOOTS AND ADVENTITIOUS ROOTS IN Eucalyptus grandis Hill ex Maiden IN VITRO

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The response of explants in a system of tissue culture is dependent on the genotype of the material in culture. The genetic variation among and within open pollination families of a 27 years old population of Eucalyptus grandis, for proliferation of shoots and production of root in vitro was studied. The test was carried out in randomized blocks, with seven treatments (progenies), with five replications and eight explants for pots. Progenies showed highly significant differences in relation to the formation of shoots and roots. Genetic variations occurred among progenies with high variation of shoots formed. Wide genetic variations within progenies also occurred for the capacity of production of shoots. The effects of the genotype and of the enviroment were significant in the formation of shoots and roots. Through selection of families, the production of shoots and of adventitious roots can be increased to optimize the multiplication in vitro that specie.

Key words
micropropagation; genetic variation; in vitro rooting

Tabela 1 Número médio de gemas formadas mediante cultivo in vitro, em meio de multiplicação MS (Murashige & Skoog, 1962) modificado .em diferentes progênies de Eucalyptus. grandis, em três subcultivos.

Progênie	Subcultivo I	Taxa Progênie	de Subcultivo II	Progênie	Multiplicação Subcultivo III	Totais Progênie N^°gemas
P3	5,8 a	P10	5,2 a	P10	6,8 a	P10 525
P6	5,8 a	P3	5,2 a	P6	6,4 a b	P6 519
P10	5,5 a b	P6	5,2 a	P3	6,0 b	P3 487
P2	5,0 b	P2	4,8 a b	P2	5,2 c	P2 378
P1	4,4 c	P4	4,4 b c	P4	4,3 d	P4 280
P4	4,2 c	P9	4,1 c	P9	4,3 d	P1 268
P9	3,9 c	P1	4,1 c	P1	4,2 d	P9 254
C.V(% )	5,8	C.V(% )	5,2	C.V(% )	6,6
DMS	0,58	DMS	0,49	DMS	0,71

Tabela 2 Análise da capacidade de enraizamento e formação de calos, aos 10 e aos 20 dias de cultivo, em gemas alongadas de progênies de Eucalyptus grandis.

Progênie	Enraizamento aos 10 dias (%)	Progênie	Formação calos aos 10 dias (%)	Progênie	Enraizamento aos 20 dias (%)	Progênie	Formação calos aos 20 dias (%)
P10	47 a	P1	23 a	P10	81 a	P9	41 a
P3	45 a	P4	23 a	P6	72 a b	P4	38 a
P6	38 a b	P2	19 a	P1	60 b c	P2	37 a
P1	36 a b	P3	13 a	P3	60 b c	P1	30 a b
P4	34 a b	P6	13 a	P2	53 c	P3	30 a b
P2	26 b	P9	12 a	P4	53 c	P6	19 b c
P9	24 b	P10	9,0 a	P9	49 c	P10	9,0 c
C.V(%)	26	C.V(%)	65	C.V(%)	14	C.V(%)	31
DMS	19	DMS	21	DMS	18	DMS	18

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