Impact of a chromosome X STR Decaplex in deficiency paternity cases

SHORT COMMUNICATION

Impact of a chromosome X STR Decaplex in deficiency paternity cases

Aluisio Trindade-Filho^III; Samuel Ferreira^III; Silviene F. Oliveira^IIIV

^ILaboratório de Antropologia Forense, Instituto de Medicina Legal, Polícia Civil do Distrito Federal, Brasília, DF, Brazil

^IIPrograma de Pós-Graduação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade de Brasília, Brasília, DF, Brazil

^IIIInstituto de Pesquisa de DNA Forense, Polícia Civil do Distrito Federal, Brasília, DF, Brazil

^IVLaboratório de Genética, Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil

^{Send correspondence to}

ABSTRACT

Deficiency paternity cases, characterized by the absence of the alleged father, are a challenge for forensic genetics. Here we present four cases with a female child and a deceased alleged father in which the analysis of a set of 21 or 22 autosomal STRs (AS STRs) produced results within a range of doubt when genotyping relatives of the alleged father. Aiming to increase the Paternity Index (PI) and obtain more reliable results, a set of 10 X-linked STR markers, developed by the Spanish and Portuguese Group of the International Society for Forensic Genetics (ISFG), was then added. Statistical analysis substantially shifted the results towards the alleged fatherhood in all four cases, with more dramatic changes when the supposed half-sister and respective mother were the relatives tested.

Keywords: forensic genetics, ChrX STR Decaplex, X-linked markers, deficiency paternity case, Paternity Index.

In the laboratory of the Brazilian Federal District Civil Police, about 11% of civil paternity investigation cases are characterized by "deficiency", i.e. the alleged father is absent. In such cases, close relatives make up for this absence by providing material for analysis, with the disadvantage that the Combined Paternity Index (PI) obtained is invariably lower than the index obtained if the alleged father is available for analysis. Furthermore, in some cases the exclusive use of autosomal STR markers may be insufficient to obtain PI values above an acceptable limit.

The introduction of X chromosome short tandem repeats (ChrX STR) in forensic genetics has a recent history (Szibor et al., 2003; Shin et al., 2005; Szibor, 2007). Markers located on this chromosome have a particular inheritance pattern: women are dizygous and men are hemizygous, with the latter receiving their single X from the mother. This makes these markers particularly suited in deficiency paternity cases. ChrX STRs can, however, only be used in paternity case disputes involving daughters, as there is no allele inherited by descent in a father-son relationship. As ChrX STRs are located on a single chromosome, investigators must take the proper precautions when using these markers for genotyping and need to take into consideration genetic linkage and possible linkage disequilibrium amongst them (Edelmann et al., 2004, Gomes et al., 2011; Pinto et al., 2011).

Here we present the use of this class of markers in four paternity deficiency cases, all including a female child and a deceased alleged father who was not analyzed. The number of subjects genotyped in each case was variable. Figure 1 displays the pedigrees of the families along with the probands that were identified by arrows. All individuals shown in the pedigrees were tested for autosomal (AS) and X STRs except for the putative uncle in family 1 and the putative grandfather in family 2, who were both genotyped only for AS STRs. All cases were previously analyzed for either 21 or 22 AS STRs using the AMPFISTR Identifiler^® (Applied Biosystems, CA, USA), Powerplex^® 16 System, GenePrint FFFL Multiplex kit and PowerPlex^® ES Monoplex System SE33 (Promega Corp., Madison, WI, USA) amplification systems. The results of the four cases failed to achieve PI values equal to or greater than 10,000, this being the cut off point set according to the laboratory of the Brazilian Federal District Civil Police internal protocol, considering for the alternative hypothesis an unrelated person (Table 1). Genotypes and frequencies of the allele for AS STRs transmitted by the deceased father are presented in Supplementary material Table S1, and the formulae used to calculate PIs, considering the profile configurations depicted with AS STRs in the four cases, are presented in Tables S2-S5).

These four cases were then analyzed with the X-Decaplex system, composed of 10 ChrX STRs: DXS8378, DXS9898, DXS7133, GATA172D05, GATA31E08, DXS7423, DXS6809, DXS7132, DXS9902 and DXS6789 (Gusmão et al., 2009). These STRs are located in three of the four linkage groups described by Szibor et al. (2003) for the X chromosome.

Briefly, the X STR laboratory analysis protocol was as follows: DNA was obtained from blood samples and/or oral swab employing the rapid NaOH extraction method (Richards et al., 1993). Genomic DNA (1 ng) was amplified in a 10 /L total volume of PCR reaction mix in a GeneAmp 9600 thermocycler (Perkin-Elmer). The products were loaded into a 3130xl Genetic Analyzer (Applied Biosystems, CA, USA). Data was collected by DataCollection v3.0 software and alleles were assigned using Gene-Mapper v3.2.

The statistical parameters analyzed were the Paternity Index (PI) and Probability of Paternity (PP), with the assumption of an a priori probability of 0.5 for the latter. Genotypes and frequencies of the allele for X-liked STRs transmitted by the deceased father are shown in Table S6. The formulae used when the putative grandmother was the genotyped relative (cases 1 and 2) can be seen in Table 2.

When a putative half-sister was genotyped to reconstruct the putative father's haplotype (cases 3 and 4), statistical analyses were performed according to the formulae proposed by Ayres and Powley (2005). The formulae were modified in order to exclude sub-structure parameters (Table 3). In case 4, the haplotype of the alleged father could not be reconstructed at three markers (DXS8378, DXS7423 and DXS6789), where the alleged half-sister and her mother shared the same heterozygous genotype. For each of these three markers, the formula fa/fa+fb was used to estimate the probability of the paternal obligatory allele, also identified in the allelic profile of the daughter, as being part of the haplotype of the alleged father. In this formula, fa denotes the frequency of occurrence of the paternal obligatory allele identified in both the genotype of the daughter and the genotype of the putative half-sister, and fb denotes the other allele, identified in the profile of the latter. The value obtained was used as the probability of the alleged father has forwarded the paternal mandatory allele for the daughter, in the hypothesis of prosecution (HP) for each marker. The allelic frequencies used were the weighted average frequencies of four Brazilian populations obtained from Gusmão et al., 2009. A Microsoft Office 2007 Excel Worksheet (Microsoft Corporation) was used to perform the calculations of PI and PP for both classes of markers. In case 3, the genotypes of the putative grandmother and the one motherless alleged half-sister were used only to identify the paternal allele of the putative deceased father in a locus where the other biological half-sister and her mother shared the same heterozygous genotype.

A first result was that we observed no inconsistencies by genotyping the four cases with the X-Decaplex system. Table 1 shows the statistical evaluation of the four cases for each class of marker separately, and for both classes together. In cases 1 and 2, the paternity index obtained by adding the ChrX-STRs data (4.19 for case 1 and 51.35 for case 2) still remained substantially lower than the cut off point according to the laboratory of the Brazilian Federal District Civil Police internal protocol. It should be pointed out that in case 1, the AS STRs produced a PI of only 0.0054 and in case 2, despite an initial PI of 10.01, the mother of the tested female was not available for analysis.

In case 3, the PI based on 21 autosomal markers reached 7,000. In order to raise the PI to 10,000, the respective samples were amplified for the autosomal marker SE33. An inconsistency in a repeat unit was, however, found between the putative father's rebuilt genotype and that of the tested child. The inconsistency was interpreted as a mutation, this moving the PI to 56. By incorporating additional information from X-Decaplex it was possible to achieve a final PI close to 9,000,000, consistent with paternity. The most dramatic change occurred in case 4, where a PI of less than 1,000 achieved by genotyping 21 autosomal STRs rose to a final PI close to 1,500,000,000 after joining the PI of the 10 ChrX markers.

Due to inheritance patterns, STRs located on chromosome X are well suited to evaluate cases of deficiency paternity. In these scenarios, the product rule depends on the absence of a linkage disequilibrium between the loci tested in the population of interest (Gusmão et al., 2009). In a joint study carried out by the Spanish and Portuguese ISFG Working Group (ISFG), no linkage disequilibrium was observed between the loci of the X-Decaplex system in 15 Ibero-American populations, four of which were from Brazil (Gusmão et al., 2009). Additionally, no linkage disequilibrium was observed in the Brazilian Federal District population, which includes the tested individuals (Trindade-Filho, unpublished data).

If we assume that the individuals analyzed are inserted within a population in linkage equilibrium, then the 10 loci of the X Decaplex system could be considered in the statistical evaluation for all four cases. In cases 3 and 4, the linkage equilibrium alone allowed the authors to use the product rule to estimate the frequency of occurrence of the reconstructed haplotype of the alleged father.

For cases 1 and 2, the estimate of the frequency of occurrence of the reconstructed haplotype of the alleged father is more complex. When ChrX markers are investigated in a deficiency paternity case, the putative grandmother plays the same role as the putative father when autosomal STRs are used, and the statistical evaluation should be made under the same principle if two STRs are closely linked. Here we refer to the principle that says: if two markers do not segregate independently, the product rule can get undermined. This is the picture in cases 1 and 2, as the 10 X markers are located in three of the four linkage groups above mentioned. Nevertheless, we followed the guidance of Gill et al. (2012) who argue that if one assumes a linkage equilibrium at the population level, the effects of linkage in pedigrees spanning a few generations in forensic caseworks has no effect at all, unless at least one individual is involved in at least two transmissions of genetic material, either as a parent or a child, and that individual is a double heterozygote at the loci in question. And this are not the issues in our pedigrees.

Our results are in agreement with the literature and demonstrate the usefulness of ChrX STRs in selected cases of deficiency paternity (Edelmann et al., 2004; Aquino et al., 2009), especially if biological daughters of the alleged father and their mothers are available for reconstructing his haplotype. If the profile of the alleged father can be reconstructed, it should be emphasized that the haplotype from ChrX markers is more informative than the genotype from autosomal markers (Szibor et al., 2003), considering the same allele frequencies and degree of polymorphism among the loci tested.

Acknowledgments

We would like to thank Eliza Cerveira for her valuable discussion of the paper and English revision.

Supplementary Material

The following online material is available for this article:

Table S1 -Genotypes for AS-STRs and frequencies of the paternal obligatory alleles (APO) identified in the profile of the daughter

Table S2 -Formulae to calculate PI considering the profile configurations depicted with AS STRs, in case 1.

Table S3 -Formulae to calculate PI considering the profile configurations depicted with AS STRs, in case 2.

Table S4 -Formulae to calculate PI considering the profile configurations depicted with AS STRs, in case 3.

Table S5 -Formulae to calculate PI considering the profile configurations depicted with AS STRs, in case 4.

Table S6 -Genotypes for X-liked-STRs and frequencies of the paternal obligatory alleles (APO) identified in the profile of the daughter.

This material is available as part of the online article from http://www.scielo.br/gmb.

Received: November 26, 2012

Accepted: October 14, 2013.

Associate Editor: Maria Rita Passos-Bueno

License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

• Aquino J, Peixe C, Silva D, Tavares C and Carvalho EF (2009) A X-chromosome STR hexaplex as a powerful tool in deficiency paternity cases. Forensic Sci Int Genet Suppl Ser 2:45-46.
• Ayres KL and Powley WM (2005) Calculating the exclusion probability and paternity index for X-chromosomal loci in the presence of substructure. Forensic Sci Int 149:201-203.
• Edelmann J, Lessig R, Klintschar M and Szibor R (2004) Advantages of X chromosomal microsatellites in deficiency paternity testing: Presentation of cases. Int Congr Ser 1261:257-259.
• Gill P, Phillips C, McGovern C, Bright J and Buckleton J (2012) An evaluation of potential allelic association between the STRs vWA and D12S391: Implications in criminal casework and applications to short pedigrees. Forensic Sc Int Genet 6:477-486.
• Gomes C, Magalhães M, Amorim A, Alves C, Pinto N and Gusmão L (2011) How useful is your X in discerning pedigrees? Forensic Sci Int Genet Suppl Ser 3:161-162.
• Gusmão L, Sánchez-Diz P, Alves C, Gomes I, Zarrabeitia MT, Abovich M, Atmetlla I, Bobillo C, Bravo L and Builes J (2009) A GEP-ISFG collaborative study on the optimization of an X-STR Decaplex: Data on 15 Iberian and Latin American populations. Int J Legal Med 123:227-234.
• Pinto N, Gusmão L and Amorim A (2011) X-chromosome markers in kinship testing: A generalisation of the IBD approach identifying situations where their contribution is crucial. Forensic Sci Int Genet 5:27-32.
• Richards B, Skoletsky J, Shuber AP, Balfour R, Stern RC, Dorkin HL, Parad RB, Witt D and Klinger KW (1993) Multiplex PCR amplification from the CFTR gene using DNA prepared from buccal brushes/swabs. Hum Mol Genet 2:159-163.
• Shin SH, Yu JS, Park SW, Min GS and Chung KW (2005) Genetic analysis of 18 X linked short tandem repeat markers in Korean population. Forensic Sci Int 147:35-41.
• Szibor R (2007) X-chromosomal markers: Past, present and future. Forensic Sci Int Genet 1:93-99.
• Szibor R, Krawczak M, Hering S, Edelmann J, Kuhlisch E and Krause D (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67-74.

Publication Dates

• Publication in this collection
  09 Dec 2013
• Date of issue
  2013

History

• Received
  26 Nov 2012
• Accepted
  14 Oct 2013