Three culture media in combination with distinct accessions, crossing generations and periods of time after artificial pollination were evaluated in order to identify more efficient protocols to recover interspecific hybrids between Lycopersicon esculentum and L. peruvianum. Both type of media for seed plating and the interval time for fruit harvest after artificial pollination had significant influence on the recovery of interspecific hybrids. The HLH medium gave the best results. The interval between 25 and 35 days after the artificial pollination was found to be the ideal for recovering interspecific hybrids. For acclimation, the L. esculentum and L. peruvianum plants were evaluated as well as plants of the interspecific hybrids (F1) and those from the RC1 and RC2 generations. This process was affected by the size of the buds used in the seedling transfer before acclimation as well as by the accesses and the time period over which the plantlet was in the test tube. The hybrids presented a better acclimation ability. The ideal period ranged between 26 and 35 days after transferring the seedling to the test tube. The acclimation accomplished under mild environmental conditions improved the plantlet survival.
culture media; interespecific hybrids; acclimatization
