Germinação e crescimento inicial de três espécies de leguminosas em substrato de área minerada: efeitos na alocação de biomassa e nodulação

Figueiredo, Maurílio Assis; Messias, Maria Cristina Teixeira Braga; Leite, Mariangela Garcia Praça; Kozovits, Alessandra Rodrigues

doi:10.1590/2236-8906e162024

conceived and designed the research · performed experiments · analyzed data · wrote and edited the manuscript

schoolUniversidade Federal de Ouro Preto, Postgraduate Program in Crustal Evolution and Natural Resources, Campus Morro do Cruzeiro, Bauxita, 35402-206 Ouro Preto, MG, Brazil

0000-0003-2095-4680

person Maria Cristina Teixeira Braga Messias

wrote and edited the manuscript

schoolUniversidade Federal de Ouro Preto, Department of Biodiversity, Evolution and Environment, Campus Morro do Cruzeiro, Bauxita, 35402-206 Ouro Preto, MG, Brazil

0000-0003-0300-9038

person Mariangela Garcia Praça Leite

supported resources for laboratory analysis · wrote and edited the manuscript

schoolUniversidade Federal de Ouro Preto, Department of Geology, Campus Morro do Cruzeiro, Bauxita, 35402-206 Ouro Preto, MG, Brazil

0000-0002-9789-3786

person Alessandra Rodrigues Kozovits

supported resources for laboratory analysis · wrote and edited the manuscript

schoolUniversidade Federal de Ouro Preto, Department of Biodiversity, Evolution and Environment, Campus Morro do Cruzeiro, Bauxita, 35402-206 Ouro Preto, MG, Brazil

0000-0002-7312-0076

personCorresponding author: emailmaurilioafigueiredo@ufop.edu.br

¹

Part of the PhD thesis of the first Author

Associate Editor:

Nelson Augusto dos Santos Júnior

Conflict of interests

The authors declare that they have no conflict of interest regarding the publication of this article.

Figuras (5)
Tabelas (2)

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Figure 1 Germinability (mean ± SD) of S. reniformis, C. mucronata and C. coriaceum in bauxite mine substrate in the treatments: control (Contr), chemical scarification (Chem) and mechanical scarification (Mech.). Different letters above the columns of each species, mean significant differences between treatments. Tukey Test (p value <0.05).

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Figure 2 Total dry biomass (a), root/shoot ratio of dry biomass (b) and dry biomass of nodules (c) (mean ± SD) of S. reniformis, C. mucronata and C. coriaceum after 70 days of planting in pots with treatments: 1:1 mixtures of mine substrate plus commercial substrate (MS + CS), addition of 3.3% of commercial substrate to mine substrate (CS), addition of 6.6% topsoil to mine substrate (TS), mine substrate (MS). Different uppercase and lowercase letters above the columns indicate, respectively, significant differences among treatments in each species and among species in the same treatment. Mann-Whitney (p value <0.05). Only one S. reniformis individual from the TS treatment had one nodule (1.2 mg).

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Figure S1 Dry biomass gain percentage of Senna reniformis, Chamaecrista mucronata and Centrosema coriaceum species after 70 days of planting in pots with treatments: 1: 1 mixture of mine substrate plus commercial substrate - MS + CS; addition of 3.3% of commercial substrate to mine substrate - CS; addition of 6.6% topsoil to mine substrate - TS; mine substrate - MS. Different letters represent significant differences between treatments (p value <0.05).

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Figure S2 Seedlings of the three species under study in a mine substrate germination experiment (a). Detail of Senna reniformis (b), Chamaecrista mucronata (c) and Centrosema coriaceum (d) individuals 70 days after planting in pots in the mine substrate plus commercial substrate treatment. S. reniformis (e) and C. coriaceum (f) individuals 70 days after planting in pots.

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Figure S3 Details of nodules of nitrogen-fixing bacteria in Centrosema coriaceum (a) and Chamaecrista mucronata roots (b). Comparison of images showing the difference in investment in root biomass in C. mucronata plants. Plant grown in the 1: 1 mixture of mine substrate plus commercial substrate (c) and plant grown in mine substrate (d).

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Table S1 Fertility of substrates topsoil -TS, commercial substrate - CS, mine substrate: MS, mine substrate and commercial substrate: MS + CS. Total concentration of chemical elements and percentage grain-size fractions in the mine substrate. SB: sum of bases, t: effective cation exchange capacity, T: cation exchange capacity in pH 7.0, V: base saturation, m: aluminum saturation, OM: organic matter, P-rem: remnant phosphorus, CS: coarse sand, FS: fine sand, S: silt, C: clay.

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Table S2 Average and standard deviation of number of leaves (No. of leaves), shoot length, root extension (determined by Winrhizo), dry shoot and root biomass, percentage of plants with nodules (PN (%)) and average number of nodules per plant (No. of nodules) in the different treatments. 1: 1 mixture of mine substrate plus commercial substrate - MS + CS; addition of 3.3% of commercial substrate to mine substrate - CS; addition of 6.6% topsoil to mine substrate - TS; mine substrate - MS. Data followed by different letters in the same column represent significant differences between treatments.

Fertility Parameters and units of measure						Total concentration of chemical elements mg/kg)				Grain size (%)
		TS	CS	MS	MS+CS	MS		MS		MS
pH		4.52	6.37	4.88	6.27	Ca	218	Al	147561	CS	10.2
P	mg/dm³	5.10	627	0.40	290	Mg	518	As	71	FS	4.8
K	mg/dm³	35	1545	0.00	753	K	354	Ba	29	S	5.8
Ca²⁺	cmolc/dm³	0.49	7.35	0.23	5.22	P	396	Co	19	C	79.2
Mg ²⁺	cmolc/dm³	0.24	8.78	0.02	4.97	S	243	Cr	346
Al3+	cmolc/dm³	0.78	0.00	0.09	0.00	Cu	17	Na	241
H+Al	cmolc/dm³	9.20	4.20	3.30	3.90	Fe	174518	Ni	49
SB	cmolc/dm³	0.82	20.1	0.25	12.1	Mn	410	Sc	14
t	cmolc/dm³	1.60	20.1	0.34	12.1	Mo	5	Sr	59
T	cmolc/dm³	10.0	24.3	3.55	16.0	Zn	36	Th	36
V	%	8.20	82.1	7.00	75.7			Ti	14095
m	%	48.8	0.00	26.5	0.00			V	325
OM	dag/kg	3.59	32	1.07	9.13			Y	24
N	dag/kg	0.11	0.54	0.03	0.19			Zr	459
P-rem	mg/L	35.5	45.0	13.3	29.0

		Length (cm)		Dry Biomass (g)		Nodules
	No of leaves	Shoot	Root	Shoot	Root	PN(%)	No of nodules
Senna reniformis
MS+CS	5.25 ± 1.09a	15.80 ± 1.79a	604 ± 210a	0.70 ± 0.22a	0.16 ± 0.07a	0.00	0.00 ± a
CS	4.63 ± 0.48a	8.25 ± 1.30b	333 ± 90b	0.18 ± 0.08a	0.08 ± 0.04b	0.00	0.00 ± a
TS	3.38 ± 0.48b	6.25 ± 0.43c	222 ± 65b	0.11 ± 0.03a	0.06 ± 0.03b	12.5	0.13 ± 0.33a
MS	3.25 ± 0.43b	6.38 ± 0.70c	382 ± 84b	0.13 ± 0.03b	0.09 ± 0.03b	0.00	0.00 ± a
Chamaecrista mucronata
MS+CS	9.43 ± 3.02a	27.9 ± 6.69a	211 ± 86a	0.47 ± 0.18a	0.08 ± 0.03a	14.2	0.14 ± 0.35b
CS	6.86 ± 1.64ab	16.3 ± 5.62b	243 ± 44a	0.21 ± 0.08b	0.06 ± 0.02a	100	6.00 ± 3.46a
TS	6.00 ± 1.29b	13.0 ± 3.42bc	226 ± 65a	0.18 ± 0.08b	0.07 ± 0.02a	100	4.67 ± 2.05a
MS	3.86 ± 0.99b	8.00 ± 2.51c	318 ± 113a	0.12 ± 0.03b	0.07 ± 0.02a	100	4.57 ± 2.13a
Centrosema corieaceum
MS+CS	4.38 ± 0.99a	11.6 ± 2.18a	447 ± 186a	0.30 ± 0.13a	0.08 ± 0.04a	25	0.38 ± 0.70c
CS	3.75 ± 0.83a	8.38 ± 1.11b	461 ± 152a	0.13 ± 0.04b	0.07 ± 0.02ab	100	6.38 ± 3.90a
TS	2.43 ± 0.49b	7.00 ± 1.41bc	333 ± 92a	0.06 ± 0.01c	0.04 ± 0.01b	14.2	0.14 ± 0.35c
MS	2.13 ± 0.33b	5.13 ± 0.60c	382 ± 102a	0.05 ± 0.02c	0.04 ± 0.01b	100	2.88 ± 1.05b

Germinação e crescimento inicial de três espécies de leguminosas em substrato de área minerada: efeitos na alocação de biomassa e nodulação

RESUMO