Cellular proliferation markers in peripheral and central fibromas: a comparative study

Garcia, Bruna Gonçalves; Caldeira, Patrícia Carlos; Johann, Aline Cristina Batista Rodrigues; Sousa, Suzana Cantanhede Orsini Machado de; Caliari, Marcelo Vidigal; Carmo, Maria Auxiliadora Vieira do; Mesquita, Ricardo Alves

doi:10.1590/1678-7757201302116

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Journal of Applied Oral Science

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Original Articles • J. Appl. Oral Sci. 21 (2) • Mar-Apr 2013 • https://doi.org/10.1590/1678-7757201302116 copy

Cellular proliferation markers in peripheral and central fibromas: a comparative study

Authorship SCIMAGO INSTITUTIONS RANKINGS

Objective:

To perform a comparative study of the cellular proliferation in the peripheral and central fibromas.

Material and Methods:

Immunohistochemistry for PCNA and the AgNOR technique were performed in 9 cases of peripheral odontogenic fibroma (POF), in 4 cases of odontogenic fibroma (OdF), in 8 cases of peripheral ossifying fibroma (PEOF) and 7 cases of ossifying fibroma (OsF). The Kruskal-Wallis and Mann-Whitney tests were used for the statistical analyses.

Results:

Mesenchymal component of the central lesions presented a higher mean number of AgNOR per nucleus and PCNA index than did the peripheral lesions (P≤0.05). The mean number of AgNOR per nucleus in the epithelial component proved to be higher in the OdF than in the POF (P≤0.05). The mesenchymal and epithelial components presented similar mean numbers of AgNOR per nucleus and PCNA index in the OdF, as well as a similar mean number of AgNOR per nucleus in the POF.

Conclusions:

The mesenchymal component may well play a role in the differences between the biological behaviour of the central lesions as compared to the peripheral lesions. Moreover, considering that the epithelial and mesenchymal components in odontogenic fibromas presented a similar proliferation index, more research is warranted to understand the true role of the epithelial components, which are believed to be inactive in nature, as well as in the development and biological behaviour of these lesions.

AgNORs; Odontogenic tumours; Ossifying fibroma; PCNA

	POF mean (min/max)	OdF mean (min/max)	P value^a a Mann-Whitney test. * Statistically significant values (P≤0.05)	PEOF mean (min/max)	OsF mean (min/max)	P value^a a Mann-Whitney test. * Statistically significant values (P≤0.05)
Mean number of AgNOR per nucleus^b b Kruskal-Wallis test: H=113880, p=0.0098	1.26 (1.10/1.50)	1.49 (1.40/1.70)	0.0136^*	1.25 (1.10/1.40)	1.48 (1.30/1.70)	0.0428^*
AgNOR area (µm2)^c c Kruskal-Wallis test: H=115380 p=0.0091	1.46 (1.40/1.90)	1.21 (1.10/1.40)	0.0308^*	1.62 (1.39/1.86)	1.25 (1.00/1.53)	0.0128^*
IP^d d Kruskal-Wallis test: H=162612 p=0.0010	43.6 (11.6/58.2)	61.2 (56.0/67.6)	0.0087^*	47.6 (40.2/55.6)	57.1 (50.8/62.8)	0.0038^*

	POF mean (min/max)	PEOF mean (min/max)	P value^a a Mann-Whitney test. * Statistically significant values (P≤0.05)	OdF mean (min/max)	OsF mean (min/max)	P value^a a Mann-Whitney test. * Statistically significant values (P≤0.05)
Mean number of AgNOR per nucleus	1.26 (1.10/1.50)	1.25 (1.10/1.40)	0.9233	1.49 (1.40/1.70)	1.48 (1.30/1.70)	0.5708
AgNOR area (µm²2 Allison RT, Spencer S. Nucleolar organizer regions in odontogenic cysts and ameloblastomas. Br J Biomed Sci. 1993;50:309-12.)	1.46 (1.40/1.90)	1.62 (1.39/1.86)	0.0922	1.21 (1.10/1.40)	1.25 (1.00/1.53)	0.8501
IP	43.6 (11.6/58.2)	47.6 (40.2/55.6)	0.8099	61.2 (56.0/67.6)	57.1 (50.8/62.8)	0.2193

	ePOF mean (min/max)	eOdF mean (min/max)	P value^a a Mann-Whitney test. * Statistically significant values (P≤0.05)
Mean number of AgNOR per nucleus	1.25 (1.07/1.51)	1.45 (1.40/1.51)	0.0372^* * Statistically significant values (P≤0.05)
AgNOR area (µm²2 Allison RT, Spencer S. Nucleolar organizer regions in odontogenic cysts and ameloblastomas. Br J Biomed Sci. 1993;50:309-12.)	1.47 (1.30/1.65)	1.23 (1.07/1.48)	0.0449^* * Statistically significant values (P≤0.05)
IP	78.5 (34.2/99.1)	78.45 (45.8/94.6)	0.7576

	mPOF mean (min/max)	ePOF mean (min/max)	P value^a a Mann-Whitney test. * Statistically significant values (P≤0.05)	mOdF mean (min/max)	eOdF mean (min/max)	P value^a a Mann-Whitney test. * Statistically significant values (P≤0.05)
Mean number of AgNOR per nucleus	1.26 (1.10/1.50)	1.25 (1.07/1.51)	0.8946	1.49 (1.40/1.70)	1.45 (1.40/1.51)	0.5637
AgNOR area (µm²2 Allison RT, Spencer S. Nucleolar organizer regions in odontogenic cysts and ameloblastomas. Br J Biomed Sci. 1993;50:309-12.)	1.46 (1.40/1.90)	1.47 (1.30/1.65)	0.7573	1.21 (1.10/1.40)	1.23 (1.07/1.48)	0.5637
IP	43.6 (11.6/58.2)	78.5 (34.2/99.1)	0.0071^* * Statistically significant values (P≤0.05)	61.2 (56.0/67.6)	78.45 (45.8/94.6)	0.2482

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[1] Corresponding address: Patrícia Carlos Caldeira - Universidade Federal de Minas Gerais - Faculdade de Odontologia - Departamento de Clínica, Patologia e Cirurgia Odontológicas - Av. Antônio Carlos, 6627, Sala 3201 - Pampulha - 31.270-901 - Belo Horizonte - MG - Brasil - Phone: +55 31 34092476 - Fax: +55 31 34092430 - e-mail: pat_caldeira@yahoo.com.br