Synthesis and Trypanocidal Activity of 7 , 2 ’-Dioxygenated Isoflavones and Oxypropanolamine Derivatives

7,2’-Di-hidroxiisoflavona apresentou atividade contra tripomastigotas sanguíneos do Trypanosoma cruzi, agente etiológico da doença de Chagas, o que motivou a síntese de derivados anfifílicos de potencial utilização como quimioprofilático para esterilização de sangue para transfusão. Esta substância foi obtida por desmetilação da 7-hidroxi-2’-metoxiisoflavona, sintetizada via desoxibenzoína, seguindo-se eterificação com epicloridrina e abertura do anel oxirânico com dietilamina para levar à 7-oxipropanolamina, a qual, por tratamento com iodeto de metila, forneceu o sal de amônio correspondente. Nos ensaios in vitro com tripomastigotas sanguíneos do T. cruzi, a 7-hidroxi-2’-metoxiisoflavona foi inativa e a 7-oxipropanolamina foi mais ativa do que a 7,2’-di-hidroxiisoflavona. Já o sal de amônio, não só não eliminou completamente o parasita do sangue como causou lise total dos eritrócitos. Portanto, o sal de amônio não é adequado para o uso como profilático na transmissão do T.cruzi por transfusão sangüínea. A atividade tripanossomicida da 7,2’-di-hidroxiisoflavona e do seu derivado 7oxipropanolamina estimulam a avaliação in vivo destas substâncias como potenciais quimioterápicos para tratamento da doença de Chagas, sendo necessários alguns gramas destas substâncias que poderão ser obtidos pelos processos simples descritos neste trabalho.


Introduction
Chagas ' disease is a serious health problem in Central and South America.It has been estimated that 16-18 million   people are infected in Latin America and 100 million are exposed to the risk of infection. 1,3Despite the identification of the hemoflagellate protozoan Trypanosoma cruzi as the etiologic agent of this disease in 1909, only two drugs were marketed, in the period of 1975 to 1994, for the clinical treatment of the infection, nifurtimox (nitrofuran) and benznidazole (nitroimidazole) which are effective only in the acute phase and in recent chronic infection.
Benznidazole is actually the only available drug since nifurtimox has had its commercialization discontinued. 3In Brazil, transmission of T. cruzi by insect vectors is under control and transmission by blood transfusion is now an important mechanism of infection. 2Gentian violet is recommended as prophylatic drug in blood banks of endemic areas, but there are some restrictions on its use. 3 The lack of effective drugs for treatment of Chagas' disease and to prevent blood transmission creates a need to find new trypanocidal agents. 41][12] However, the water insolubility of most of these compounds is a serious limitation to their use for sterilization of blood for transfusion.Considering previous results on the trypanocidal effect of isoflavonoids 11 we decided to carry on the synthesis of 7,2'-dioxygenated isoflavones and oxypropanolamines derivatives for in vitro assays against bloodstream trypomastigotes of T. cruzi.

Results and Discussion
The oxypropanolamine 6 and its ammonium salt 7, an amphiphilic cationic derivative, were prepared as shown in Figure 1.The 7-hydroxy-2'-methoxyisoflavone (3) was synthesized by the Wähälä and Hase one-pot procedure that starts with appropriately substituted phenols and phenylacetic acids via intermediate deoxybenzoins (1,2diarylethanones). 13 Previously, the cyclization of hydroxydeoxybenzoins to the corresponding isoflavones was carried on by reaction with boron trifluoride etherate and methanesulphonyl chloride in DMF. 14 In the one-pot procedure for the synthesis of polyhydroxyisoflavones the intermediate deoxybenzoins are obtained by Friedel-Crafts reaction using boron trifluoride etherate as both catalyst and solvent, the cyclization occurring in situ by addition of DMF and mesyl chloride. 13The cyclization reaction of deoxybenzoin is also catalyzed by boron trifluoride, which is believed to form a complex with 2-hydroxyarylketones.The complexation deactivates the polyoxygenated aromatic ring preventing ring formylation and consequent polymerization while the methylene function is activated for formylation. 13Therefore, the isoflavone 3 was prepared in good yield (83%) from resorcinol (1) and 2'methoxyphenylacetic acid (2) (Figure 1).Demethylation of 3 with aluminium chloride 15 gave the 7, 2'-dihydroxyisoflavone (4) (84% yield).The oxypropanolamine 6, that would lead to the ammonium salt 7, was synthesized by a reaction sequence that started with the preparation of the glycidic ether 5 (40% yield) from the corresponding phenoxide ion formed in situ, by treatment of 7,2'dihydroxyisoflavone (4) with NaH and subsequent reaction with epichlorohydrin. 16The selective etherification of the 7-hydroxyl group has occurred as expected since it is the most acidic in an isoflavone molecule due to the conjugation with the carbonyl group. 15Subsequently, the oxirane ring opening of 5 with diethylamine yielded, as expected, 17 the aryloxypropanolamine 6 (51% yield) that by reaction with methyl iodide, in acetone, gave the desired amphiphilic cationic derivative, the ammonium salt 7 (21% yield).
The isoflavones 3 and 4 are known compounds and were characterized by their melting points and spectroscopic data that are in agreement with those previously reported. 18All the other substances obtained are novel chemical entities and have had their structures confirmed by IR, MS, 1 H and 13 C NMR spectral data.The assignments were based on 2D NMR experiments that were particularly useful for confirming the location of the glycidic ether chain of 5 at C-7 and not in C-2'.Two-dimensional nuclear Overhauser effect spectroscopy (NOESY) revealed the dipolar cross relation between both oxymethylenes protons ( H-1"a,b) of the side chain to the aromatic protons H-6 and H-8.Coincidentally, the signals of H-5 ' and of H-3 ' were overlapped with the ones of H-8 and H-6, respectively, but a correlation between H-5' and hydrogens of the side chain would not be possible if it was located at C-2'.The n.O.e enhancement of signals of the hydrogens close to the oxymethylenes protons ( H-1''a,b), among others, were measured by NOEDIFF experiments and are shown in Table 1.The decoupled 13 C NMR spectrum of compounds 5-7 presented characteristic signals for the carbons of the side chain that were assigned via DEPT and HMQC contour plot (Table 2).Different signals were observed for each methyl (δ 8.1 and 8.1) and methylene (δ 58.5 and 58.2) carbons of the two Nethyl groups, as expected for the ammonium salt 7.Moreover, the signals of the carbons linked to the nitrogen are downfield shifted compared to the corresponding carbons of 6 (Table 2).
All the synthesized compounds were evaluated for in vitro activity against the bloodstream trypomastigote form of T. cruzi.A compound was considered as active when it caused a complete elimination of parasites from blood and inactive when parasites were still observed at the microscope after 24h at 4 o C (Table 3).All the compounds were assayed at three concentrations at which 3, 5 and 7 were inactive.The 7,2'-dihydroxyisoflavone (4) showed activity in the two higher concentrations while the 2'-O-methyl derivative (3) and the 7-O-glycidic ether 5 were inactive in all the assayed concentrations.The oxypropanolamine 6 was more active than the isoflavone 4. It was significantly effective in eliminating the parasite from blood up to the concentration of 1.13 μmol L -1 and was inactive at 0.56 μmol L -1 but it caused partial lysis of the erythrocytes even at the lowest assayed concentration.The higher activity of 6 in relation to 4 can be related to its more hydrophyllic character and possibly to the presence of its protonated form in the medium of the test.With the ammonium salt 7 a reduction in the number of T. cruzi trypomastigotes was observed and it caused a complete lysis of the erythrocytes.Therefore this compound is not useful as chemoprophylatic agent for preventing the transmission of T. cruzi by blood transfusion.On the other hand the effects of compounds 4 and 6 suggest further studies evaluating their potential use as chemotherapeutic agents in the treatment of Chagas' disease.The next step should be in vivo assays in mice experimentally infected with T. cruzi and will require gram quantities of these a positive control, gentian violet (130 μg mL -1 ); negative controls, TCM199, TCM199 + DMSO; + inactive, presence of bloodstream trypomastigotes; -active, absence of bloodstream trypomastigotes; L partial lysis of erythrocytes; TL total lysis of erythrocytes; ND not determined.
compounds that can be prepared by the simple procedures described in this paper.

Biossay
Bloodstream trypomastigotes of T. cruzi Y strain were obtained from adult male albino mice acutely infected on day 7.Each compound (at two times the molecular mass/ 100) was dissolved in 200 μL of dimethyl sulfoxide (DMSO) plus 2.0 mL of tissue culture medium199 (TC199) containing 10% heat-inactivated fetal bovine serum.Aliquots of each solution were mixed with equal volume of blood from acutely infected mice.Final working concentrations were 4.50 μmol L -1 , 2.25 μmol L -1 , 1.13 μmol L -1 and 0.56 μmol L -1 .A parasite density of 2×10 5 per 100 μL of blood was calculated for each flat-bottom test tube, as described previously. 11Control tubes were included for TCM199, TCM199 plus DMSO and gentian violet (130 μg mL -1 ).The tubes were incubated at 4 o C for 24 h.Thereafter, 5 μL of each suspension was examined microscopically for the presence of motile parasites.

Chemical synthesis
Melting points are uncorrected.NMR spectra were obtained on a BRUKER AVANCE DRX400 spectrometer with TMS as internal standard.Infrared spectra were recorded on a Shimadzu IR 408 instrument (Departamento de Química, Universidade Federal de Minas Gerais).Mass spectra were determined on a VG Autospec instrument (LEMAR-LPN-PADETEC -Universidade Federal do Ceará).DMF was distilled from phosphorus pentoxide and stored in the presence of 3Å molecular sieves.BF 3 .OEt 2 was distilled from calcium hydride.Organic extracts were dried over anhydrous sodium sulfate.The starting compound 1 was obtained from Merck and 2 from Aldrich.

Table 1 .
Quantitative Overhauser nuclear effect (%) in the 1 H NMR spectrum of 5

Table 2 .
13C NMR data for the side chain of compounds 5, 6 and 7 (100 MHz)

Table 3 .
In vitro activities of compounds 3-7 against bloodstream trypomastigotes of Trypanosoma cruzi Y strain a