Alkaloid and other Chemical Constituents from Psychotria stachyoides Benth .

Os extratos orgânicos das folhas e raízes de Psychotria stachyoides forneceram o novo alcaloide indólico monoterpênico glicosilado N-desmetil-correantosida, além de bizantionosida B, a-amirina, éter metílico da alizarina, rubiadina, escopoletina, ácido barbinévico e uma mistura de b-sitosterol e estigmasterol glicosilados. A caracterização estrutural dos compostos isolados foi estabelecida com base na espectroscopia no infravermelho (IR), espectrometria de massa (MS) e particularmente ressonância magnética nuclear (RMN) 1D e 2D.


Introduction
Indole alkaloids represent the biggest single class of all alkaloids from plants.The major groups under investigation are the ones with monoterpene (C 10 ) or nor-monoterpene (C 9 ) moieties joined to the tryptamine, which are distributed on a wide range of different structures in the Apocynaceae, Loganiaceae, Nyssaceae and Rubiaceae families.The genus Psychotria (Rubiaceae) comprises more than 1000 species distributed in tropical regions worldwide.This genus is taxonomically complex, and is particularly characterized as a prolific source of bioactive polyindole and monoterpenoid indole alkaloids.Previous phytochemical studies of species of Psychotria from the Southern Brazil have revealed the remarkable presence of glycosylated indole monoterpenoid alkaloids. 1s part of the investigative efforts to find alkaloids from Rubiaceae species of the Northeastern Brazil flora, Psychotria stachyoides (subgroup Heteropsychotria), a shrub growing predominantly in tropical and sub-tropical forests from Brazil (where it is popularly named as "erva-d'anta"), 2 has been investigated.This work reports the isolation and structural characterization of the new glucosilated monoterpene indole alkaloid named as N-demethylcorreantoside (1) from the leaves of P. stachyoides, in addition to the known bizantionoside B and a-amyrin. 3,4Chemical investigation of the roots yielded alizarin methyl-ether, 5 rubiadine, 6 scopoletin, 7 barbinevic acid 8 and a mixture of b-sitosterol and stigmasterol glucosides. 9
The presence of the monoterpenoid unit was confirmed by analysis of the 1 H, 1 H COSY NMR data.This spectrum exhibited correlations for the hydrogens at d 5.74 (H-21) and 2.64 (H-20), which in turn, showed correlation to the hydrogen at d 5.85 (H-19).The strong correlations observed for the methine hydrogen at d 4.20 (H-3) and the methylene group at d 1.98 (H-14a) and 2.10 (H-14b) suggested that the secologanin unit was attached to the C-3 carbon, as reported for other similar monoterpenoid alkaloids. 10This suggestion was supported by the 13 C NMR spectrum along with the distortionless enhancement by polarization transfer (DEPT 135), that revealed 26 carbons atoms corresponding to three methylenes, one oxymethylene and one vinylidene, three methines, two hemiketal methynes and four oxymethines, six monohydrogenated sp 2 carbons, and six non-hydrogenated carbons.However, the confirmation of the structure of 1 was supported by the HMQC and HMBC (heteronuclear multiple quantum coherence and heteronuclear multiple bond coherence, respectively) experiments, that revealed the unequivocal assignments of its NMR data.Further evidences of a tetrahydro-b-carboline skeleton were possible by the observed long-range correlations in the HMBC spectrum, that showed the proton at d 3.10 (2 H-5) correlated to the carbons at d 50.6 (C-3) and 117.0 (C-7), while the proton at d 2.70 (2 H-6) showed correlations to the carbon at d 117.0 (C-7) and 136.0 (C-2).In particular, the linkage of the methylene carbon C-14 with C-3 was established by the key correlations of the methylene hydrogens at d 1.98 and 2.10 (2H-14) with the carbons at d 136.0 (C-2) and 50.6 (C-3).The glucosyl moiety positioned at C-21, was supported by the correlation between the anomeric hydrogen at d 4.82 (H-1') and the carbon at d 97.5 (C-21).The structure of 1 was finally elucidated by comparing the value of the deshielded signal relative to H-12 (d 8.15), to those reported for typical monoterpenoid indole alkaloids (d ca.7.6). 12This chemical shift difference to a more deshielded position provided the clue for the determination of a seven member lactam ring derived from the intramolecular condensation of the indole nitrogen (N-1) with the original carbonyl of the methyl ester of secologanin.Comparison of these spectral features with those related to correantoside, a monoterpene indole alkaloid previously isolated from P. correae, showed high similarity, 12 with exception of the N-methyl group at N-1, present in correantoside but missing in 1.
The relative stereochemistry of 1 was established by the nuclear Overhauser spectroscopy (NOESY) experiment.In particular by the diagnostic nuclear Overhauser effect (nOe) cross-peaks observed for the hydrogen at d 1.98 (H-14a) with the hydrogens at d 2.64 (H-20), and for the hydrogen at d 2.10 (H-14b) with the hydrogen at d 5.74 (H-21), in agreement with the relative stereochemistry of correantoside. 12rom the above evidences, compound 1 was identified and named as the new N-demethylcorreantoside.The chemical structure and the observed key nOe correlations for compound 1 are presented in Figure 1.

General experimental procedures
Infrared (IR) spectra were recorded on a Perkin-Elmer FT-IR 1000 spectrometer (Waltham, USA) using KBr pellets.The NMR spectra were performed on a Bruker Avance DRX 500 spectrometer, equipped with an inverse detection probehead and z-gradient accessory working at 500.13 ( 1 H) and at 125.77 MHz ( 13 C), respectively.All pulse sequences are standard in the Bruker XWIN-NMR software, and all experiments were conducted at room temperature.The samples were dissolved in CD 3 OD (0.6 mL) and transferred to 5 mm tubes.The 1 H and 13 C chemical shifts are expressed in the d scale and were referenced to residual CH 3 OH at d 3.31 for proton and at d 49.1 for carbon.High resolution mass spectra (HRMS) were recorded on an UltrOTOF-Q mass spectrometer (LC-IP-TOF model 225-07100-34-SHIMADZU) either by positive or negative ionization modes of the ESI source.High-performance liquid chromatography (HPLC) analyses were performed on a Shimadzu chromatographer (Japan) equipped with a ternary pump (Shimadzu LC-20AT) and UV detector (Shimadzu SPD-M20A), using Phenomenex RP-18 columns (analytical: 250 × 4.6 mm, 5 μm; semi-preparative: 250 × 10 mm, 10 μm).HPLC grade acetonitrile was purchased from Tedia Co. (São Paulo State, Brazil) and the HPLC grade water was obtained by a Milli-Q purification system (Millipore, Bedford, USA).Column chromatography was performed either over silica gel 60 (EMD, 70-230 mesh) or Sephadex LH-20 (Amersham Pharmacia Biotech, Uppsala, Sweden).Thin layer chromatography (TLC) was performed on precoated silica gel aluminum sheets (Merck) and the compounds were visualized by UV detection and by spraying with Dragendorff reagent or vanillin/perchloric acid/EtOH solution, followed by heating.

Plant material
Leaves and roots of Psychotria stachyoides Benth.were collected at the Pacoti County (Ceará State, Northeast of Brazil).Voucher specimens (# 31674) were deposited at the Herbário Prisco Bezerra (EAC) and identified by Dr. Piero Giuseppe Delpret from the Institut de Recherche pour le Développement-IRD, UMR AMAP, Montpellier, France.

Extraction and isolation
Dried leaves of P. stachyoides (2.7 kg) were exhaustively extracted with EtOH (3 × 9.0 L) at room temperature.Evaporation of the solvent under vacuum yielded the ethanol extract (130.0 g).The ethanol extract was treated with 10% aqueous HCl and the acid solution was extracted with CHCl 3 (3 × 150 mL).The aqueous phase was basified with NH 4 OH to pH 9-10 and extracted with EtOAc (3 × 150 mL).The organic fractions were combined and the solvent was evaporated to yield the alkaloid fraction (2.2 g).The alkaloid fraction (2.2 g) was chromatographed on Sephadex LH-20 by elution with MeOH to afford fourty two fractions (20 mL), that were combined into five resulting fractions after TLC analysis.Flash chromatography of fraction (2) (0.43 g) using CH 2 Cl 2 /MeOH/NH 4 OH (95:4:1) as eluent with increasing polarity yielded thirty one fractions (20 mL), which were pooled together in to four resulting sub-fractions according to TLC analysis.The sub-fraction (2)(4) (47.4 mg) was submitted to semi-preparative HPLC with a C 18 column and using CH 3 CN-H 2 O (7:3) TFA 0.2% system as eluent, to obtain 1 (4.9 mg) and bizantionoside B (6.3 mg).Successive flash chromatography from the non-alkaloidal fraction (3.0 g) by elution with hexane/EtOAc (9:1) yielded a-amyrin (37.0 mg).
The EtOH extract of the roots (50.4 g) was redissolved in a mixture of MeOH:H 2 O (1:1 v/v) and submitted to liquidliquid partition with hexane, CH 2 Cl 2 , EtOAc and n-BuOH to give the correspondent four fractions.The hexane fraction (3.9 g) was re-chromatographed on Si gel by elution with hexane/EtOAc mixtures with increasing polarity to afford eighteen sub-fractions (20 mL), these fractions were combined into eleven sub-fractions according to TLC analysis.Chromatography of fraction (5) (210.0 mg) by elution with hexane/EtOAc (3:1) yielded rubiadine (4.2 mg) and alizarin methyl ether (7.1 mg), respectively.The CH 2 Cl 2 fraction (2.6 g) was re-chromatographed on Si gel by elution with CH 2 Cl 2 /MeOH mixtures with increasing polarity, to give ninety two sub-fractions (20 mL), which were combined in nine resulting fractions according to TLC analysis.Fraction (2) yielded scopoletin (11.0 mg) and (4) yielded a mixture of b-sitosterol and stigmasterol glucosides (12.4 mg).Fraction (9) was re-chromatographed on Si gel by elution with hexane/EtOAc mixture with increasing polarity to yield barbinevic acid (20.8 mg).