FIA-FAAS Method for Tannin Determination Based on a Precipitation Reaction with Hemoglobin

Um sistema em fluxo acoplado a espectrômetro de absorção atômica com chama (FIA-FAAS) foi desenvolvido para determinação de taninos em amostras de feijão guandu, explorando a reação de precipitação entre os taninos e proteínas. Extratos obtidos pela sonicação das amostras com solução de metanol 50% (v/v), foram introduzidos no sistema e induzidos à reação com hemoglobina, sendo o precipitado produzido pela reação retido em um filtro localizado no percurso analítico. Solução 1% (m/v) dodecil sulfato de sódio, fluindo em sentido inverso ao fluxo de filtração, foi empregado para solubilizar e conduzir o complexo tanino-hemoglobina ao detector, espectrômetro de absorção atômica com chama, para quantificação dos íons ferro presentes na estrutura da hemoglobina. Uma solução de ácido tânico foi utilizada para construção da curva analítica. O método proposto apresentou freqüência analítica de 30 amostras por hora, desvio padrão relativo de 9,7 % (n=10) e limite de quantificação 0,27 mg L de ácido tânico.


Introduction
The term tannin originated from "tanning", an expression which refers to tan animal skin. 1 This is possible because tannins link to collagen, which is the protein present in animal skin. 2 Thus, the ability to link and precipitate proteins is a property that defines tannins.For this reason, the reaction between tannins and protein has been used in many analytical methods for tannin determination, [3][4][5][6] especially when results that measure the correlation with the nutritional value of food are desired. 7oreover, this reaction is very specific, which minimizes interference effects in an analytical method.
Tannin-protein complexes are dissolved by detergents, that break hydrophobic interaction between them, or by high pH values that ionize hydroxyphenolic groups so that it becomes unable to maintain the hydrogen bonding. 4sually tannin-protein precipitates are dissolved using sodium dodecyl sulfate. 7ate-Smith 6 proposed the use of hemoglobin for tannin determination as a chromoprotein in a spectrophotometric methodology.Later, Schultz et al. 8 suggested this reaction for quantitative analysis of tannins and described some advantages (water soluble and some buffering ability) in using hemoglobin, compared to other proteins.On the other hand, these methods were considered difficult for routine application because they require large amounts of fresh blood and some colored compounds from samples or blood could seriously interfere in the mesurements. 7low methods have proven to be suitable for many analytical applications due to low sample and reagent consumption, high sample throughput and the feasibility of their implementation.With this kind of system, a large number of chemical manipulations may be carried out in a contamination-free system with good precision. 9The combination of flow methods and atomic spectrometry is successful because, in these system, interference effects are overcome, firstly by the exploitation of some inherent feature of flow injection (FI), such as the use of a continuous carrier stream or micro-sample volume and secondly, since interferences may be alleviated by the implementation of some pretreatment chemistry in the FI format.Many of the limitations from which atomic spectrometry procedures suffer could be overcome by the separation of the analyte species from the unwanted matrix species.Often such separation procedures also result in increased analyte mass flux into the atom source, with consequent improvements in sensitivity and detection limits. 10n this work the advantages of quantifying tannins by exploring their precipitation reaction with proteins was investigated.Color interference was prevented by the use of flame atomic absorption spectrometry (FAAS) as measurement technique.A flow injection 9 procedure was developed to automate the determinations and to improve the reaction conditions.The main parameters involved in the determination were evaluated and the system was applied to pigeon pea (Cajanus cajan [L] Millsp) samples.

Experimental
Instrumental A freeze drier (Savant model Novalyphe NL 150, New York, USA), and a freezer mill (Spex Certiprep model 6750, Metushen, NJ, USA), which operated at liquid nitrogen temperature, were used for sample drying and grinding, respectively.An ultrasound bath (Unique model USC 1400) and a centrifuge (Fanem model 204 NR, São Paulo, Brazil) were used to, respectively extract and separate the tannin from pigeon pea samples.A spectrophotometer (Femto 432, São Paulo, Brazil) was used to determine nitrogen for achieving the stoichiometric relationship between tanninhemoglobin and to perform the manual comparative assay.
The flow setup presented in Figure 1 comprised a model ISM 761 peristaltic pump (Ismatec, Zürich, Switzerland) furnished with Tygon pumping tubes and an automatic injector-commutator (Micronal, model B-352, São Paulo, Brazil), to introduce sample and hemoglobin solutions into the system.A controlled-temperature bath (Fanem, São Paulo, Brazil) was used to ensure a constant temperature (40 o C) to speed up the reaction.
The manifold, built with 0.8 mm i.d.polyethylene tubing having a non-collapsible wall and Perspex  Yshaped connectors, was coupled to a Varian SpectrAA-800 atomic absorption spectrometer (Varian, Mulgrave, Australia).An iron hollow cathode lamp from the same manufacturer was used for measurements of absorption signals, working with 5 mA of current.The analytical wavelength was the 248.3 nm iron resonance line, selected with a spectral band pass of 0.2 nm.The background was corrected with a deuterium lamp.An air/acetylene flame (13.5:2L min -1 ) was used for all measurements, which were based on peak area measured for 30 sec.

Solutions
All solutions were prepared from analytical grade reagents, and distilled, deionized water was used throughout.
In the flow diagram in Figure 1, the extraction solution (ES) was prepared by mixing absolute methanol with water 50 % (v/v). 6,7emoglobin solution (Hb) was freshly prepared by dissolution of 0.1 g of bovine hemoglobin (Sigma, St. Louis, MO, USA) in 100.0 mL of 0.05 mol L -1 Tris/HCl buffer, pH 7.0.The Tris-HCl buffer solution was prepared by solubilization of 6.05 g of tris-(hydroxymethyl) aminomethane (Reagen, Rio de Janeiro, Brazil) in approximately 800 mL of H 2 O.The pH was adjusted with 0.10 mol L -1 HCl solution to 7.0.The final volume was then made up to 1000 mL with water.Carrier solution (C) was prepared by dissolution of 0.1 g of tannic acid (Sigma) in 100.0 mL of Tris/HCl buffer (0.05 mol L -1 , pH 7.00).Diluent solution (D) was prepared by dissolution of 1.0 g sodium dodecyl sulfate (SDS -Synth, Diadema SP, Brazil) in 100.0 mL of water.
The analytical standard solutions (0.00, 200.0, 400.0, 600.0 and 800.0 mg L -1 ) were prepared by appropriate dilutions of the stock solution containing 5.0 g L -1 of tannic acid in ES medium.

Samples
Pigeon pea (Cajanus cajan [L] Millsp) leaves and pod samples were obtained from a genetic improvement experiment. 11Leaves samples, termed L1 -L6, and pods, termed P1 -P3, were harvested under ice and washed with cold distilled water.The samples were then freeze-dried after freezing with liquid nitrogen at -196 °C.The dried samples were ground in the freezer mill, after 3 min of precooling with 2 min of grinding.Three grinding cycles were performed and a cooling step of 1 min was applied between the cycles.
A mass of 0.2 g (dry weight) of ground samples received 10 mL of the extraction solution (ES).The mixture was sonicated in an ultrasonic bath for 30 min, followed by 20 min of centrifugation at 693 g.

Flow system
At the position indicated in the flow-setup in Figure 1, the sample extract (L 1 , 175 cm, about 875 µL), and hemoglobin solution (L 2 , 175 cm, about 875 µL) were aspirated.By switching the injector, the sample extract and hemoglobin aliquots were introduced into the carrier streams (C, 2.0 mL min -1 each), flowing into a confluence point.This sample zone passed through the reaction coil (B, 150 cm, about 750 µL) immersed in a water bath at 40 °C.Afterwards, the flow containing tannin-hemoglobin complex passed into a filter (F) built into glass tubing (20 cm length and 1.5 mm i.d.), filled with glass wool, where the precipitate was retained.Again switching the injector, diluent solution (D) passed through the filter, dissolving and conducting the complex to the FAAS, where Fe ions from hemoglobin were quantified.The tannin amount can then be indirectly determined.The system was experimentally optimized based on factorial plans and response surface methodologies. 12

Stoichiometric relationship determination
The stoichiometric relation between hemoglobin and tannic acid was determined through total nitrogen analysis in the precipitate formed by reaction between them.Tannic acid (1.0 g L -1 ) and hemoglobin solution (1.0 g L -1 ) were mixed at different ratios: 1:0.5, 1:1, 1:1.25, 1:1.5, 1:2, 1:3, and 1:4 (tannic acid: hemoglobin).The resulting precipitates were filtered using quantitative paper filter (Whatman 540, 8 µm and 12.5 cm diameter).Each filter paper containing the precipitate was placed in a 100 mL digestion vessel and 15 mL of concentrated H 2 SO 4 plus 0.7 g of K 2 SO 4 /CuSO 4 (1:10) catalytic mixing were added.The vessel was then transferred to a heating block and carefully heated until the beginning of the reaction.After that, the temperature was increased up to 400 °C until formation of a colorless solution, after which heating was maintained for 1 h.The blank solution was obtained by digestion of filter paper containing no sample.After digestion, the acid solutions were evaporated to obtain nearly 2 mL, cooled and then diluted to 50 mL with water.Nitrogen amount was determined in each solution by the Berthelot reaction in a previously described flow procedure. 13

Manual assay
The comparative manual assay was based on the method proposed by Schultz et al., 8 with a few modifications.Commercial bovine hemoglobin was used, instead of fresh blood.
In a centrifuge tube, 1.0 mL of sample extract, 1.0 mL of water and 1.0 mL of hemoglobin solution (1.0 g L -1 ) were mixed in a vortex mixer for 20 min and decanted.The samples were then centrifuged for 20 min at 693 g.The precipitate was separated and dissolved with 4.0 mL of 5% (w/v) SDS solution.The resulting colored solution was analyzed in the spectrophotometer at 578 nm.

Results and Discussion
The results of tannin concentration obtained using the proposed FIA-FAAS method and that obtained by using the manual assay are shown in Table 1.The results obtained with the later method were different than those obtained with the flow approach.This occurred because there were small differences among the standard absorbances in the manual procedure, which causes a lesser slope (m = 9.35 x 10 -5 ) in the analytical curve, compared with that obtained by the FIA-FAAS method (m = 5.3 x 10 -4 ).This small difference between absorbance values could induce erroneous results, mainly owing the small amount of tannin present in pigeon pea plants.
To explain the sensitivity differences, the stoichiometric relationship between tannic acid and hemoglobin was evaluated.The results are presented in Figure 2. Greater amounts of nitrogen were detected in the precipitate obtained by mixing one part of tannic acid and two parts of hemoglobin, suggesting that this is the best condition for the tannin-hemoglobin reaction.
According to Spencer et al., 14 the association of proteins and polyphenols is mainly a superficial phenomenon, and the bond efficiency is due to the fact that polyphenols are multidentate ligands able to bond simultaneously with more than one site on the protein surface through different phenolic groups.Hagerman and Butler 15 suggested that the tannin-protein interaction occur through hydrogen bonds between donor phenolic groups and the acceptor peptide.Based on these evaluations, a probable model to explain tannic acid-hemoglobin interactions is: 2 Hb (aq) + 1 tannic acid (aq) ↔ Hb-tannic acid-Hb ↓ Thus, hydrogen atoms from phenolic groups of tannic acid act as electron-donor species, and a hydrogen atom from hemoglobin as acceptor, producing a more hydrophobic compound with higher molar mass, which precipites.This stoichiometric relationship, and some information about the hemoglobin structure, could explain the differences between the sensitivity of the manual spectrophotometric assay and the FIA-FAAS method.
The hemoglobin molecule is made up of four polypeptide chains (Alpha 1, Beta 1, Alpha 2, Beta 2), non-covalently bonded to each other.Each chain holds a heme group containing one Fe 3+ ion.Consequently, there are four heme-iron complexes in a hemoglobin molecule (Figure 3).The heme-iron complexes are red, and are responsible for the red color of hemoglobin . 16 both evaluated methods, the same amount of tannic acid and hemoglobin reagent was used.Thus, for each mol of Hb-TA-Hb formed, two mols of hemoglobin are responsible for the color measured in the spectrophotometric assay, while in FIA-FAAS, for the same complex, eight mols of iron are responsible for the analytical signal in FAAS.Considering this relationship, FIA-FAAS could be four times more sensitive than the manual spectrophotometric assay, a very important characteristic, especially for samples with low amounts of tannin.
The parameters in the flow system, which interfere directly in the analytical signal, were optimized using chemometric methodologies, and the best conditions found are outlined in Figure 1.The addition of 0.1 mg L -1 of tannic acid in carrier solution improved the crystal seeding Table 1.Tannin concentration (g kg -1 of tannic acid) determined by the proposed system (FI) and by the manual approach in leaves (L1-L6) and pods (P1 -P3) of pigeon pea samples

Figure 3 .
Figure 3. On the left, scheme of hemoglobin molecule represented by four polypeptide chains (Alpha 1, Beta 1, Alpha 2, Beta 2) and heme groups represented by spheres.On the right, a two-dimensional drawing of heme coordinated to a histidine residue, which is part of the hemoglobin protein. 18