A simple and rapid liquid chromatography-ultraviolet detection (LC-UV) method has been developed and validated for quantifying (5Z,E)-3-[2-(4-chlorophenyl)-2-oxoethyl]-5-(1H-indol-3-ylmethylene)-thiazolidine-2,4-dione (PG15) in rat plasma. A C18 reversed phase column provided chromatographic separation of the analyte which was followed by UV detection at 385 nm. The method involves precipitation of PG15 from plasma and isocratic elution with methanol:water (90:10, v/v). Total elution time was 7.5 min. The proposed method was validated and showed linear correlation in the range of 62.5 to 4000 ng mL-1. The within- and between-day precision, expressed as the relative standard error, were found to be less than 15 and 10 %, respectively, for all the concentrations investigated. The accuracy, measured using the quality control samples, was in the range of 86.1-114.9 %. The applicability of the validated method was tested in a pre-clinical pharmacokinetic study of the thiazolidinone PG15.
PG15; LC-UV; rat plasma; pharmacokinetics
