1 |
Evaluate the prevalence of DENV, OROV, CHIKV, MAYV and ZIKV in patients with acute fever disease in the city of Puerto Maldonado (Peru) |
139 human serum samples |
DENV CHIKV ZIKV |
41 (29.5%) positive for arboviruses; 13 (9.35%) positive for CHIKV; nine (6.48%) positive for DENV; seven (5.03%) positive for ZIKV. The differentiation between arboviruses is precarious by clinic only |
2 |
Develop and evaluate a rapid molecular diagnostic test for ZIKV with the RT-LAMP methodology |
120 suspect samples, 90 serum/plasma and 99 urine |
ZIKV |
100% agreement between RT-LAMP and qRT-PCR, the limit of detection of qRT-PCR being the most sensitive |
3 |
Implement a new molecular method for detecting Alphavirus |
Virus strains obtained from various laboratories |
CHIKV |
Positivity in CHIKV samples. LoD greater than the reference test in the same methodology. Twelve virus species were tested and there was no cross-reactivity |
4 |
Develop and validate a qRT-PCR assay capable of detecting all CHIKV lineages |
RNA from virus culture and serum samples from Guatemala and Ecuador in July 2015 |
CHIKV |
Effective assay, mainly the strain circulating in South America. Compared to CDC USA PCR, 98% sensitivity and 100% specificity |
5 |
Implement a syndromic approach based on the use of a multiplex qPCR assay to facilitate rapid diagnosis of dengue-like syndromes in Reunion Island |
Virus strains from various laboratories and positive human plasma |
CHIKV DENV |
Evaluation and accuracy of the assay were satisfactory for CHIKV and DENV, offering reliable answers. It has been shown to be a cheaper option than simple tests for the same pathogens |
6 |
Investigate the usefulness of simple laboratory tests as predictive markers of confirmed DENV diagnoses, as well as to analyze the genotype and phylogenetics of the circulating strain in the dengue epidemic in Guang-Dong in 2014 |
1044 patients: 875 confirmed DENV cases (862 positive by NS1 and 13 by PCR) and 169 negative |
DENV |
Simple laboratory tests (leukopenia, thrombocytopenia, aminotransferases and activated thromboplastin time) are useful markers in the diagnosis of DENV |
7 |
Describe the results of analyzes of infections by arboviruses imported from Italy carried out by the National Reference Laboratory for Arboviruses (NRLA) at the Italian National Institute of Health from July 2014 to October 2015 |
Inpatient serum samples (n = 180) |
DENV CHIKV ZIKV |
Greater number of cases of DENV. There was an increase in CHIKV cases, and the first case of ZIKV was detected. In the latter, the PRNT technique was decisive for identification; the virus was negative in serology and PCR techniques (which proved to be sensitive and specific). However, only for a short period of time, limited to viremia in the body |
8 |
Review laboratory testing methodology to explore virus presence and immune response |
Blood, serum, urine, amniotic fluid, CSF, saliva, semen, breast milk and vaginal mucosa |
ZIKV |
Viremia level is low in the serum, from two to 10 days, which makes molecular diagnosis difficult and increases the number of false negatives. The case study demonstrated permanence of the virus in red blood cells for 81 days, suggesting that the use of whole blood may increase sensitivity |
9 |
Examine the diagnostic performance of real-time PCR for detection of ZIKV |
Serum and urine from individuals who travelled to Brazil, Dominican Republic and Suriname between 2015 and 2016 |
ZIKV |
Low level viremia has been shown to cause false negatives. This study found no difference between blood and urine viremias, but suggests the use of the two samples combined. Assays with NS1 are more specific and sensitive, while assays with NS3 and NS5 have low sensitivity and difficulty in specific probes, respectively |
10 |
Describe the results found by CDC USA on the efficiency of diagnostic kits for CHIKV |
Serum and plasma |
CHIKV |
For PCR, the NS2 target is more sensitive, but there is no publication on its validation. Other methodologies were effective and had no cross-reactions |
11 |
Develop and evaluate a real-time PCR assay to potentially be a point of care diagnostic tool for detecting CHIKV |
Human serum and plasma |
CHIKV |
The assay was shown to have a clinical sensitivity and specificity of 100%, a low limit of detection and only a cross- reaction with a little-known virus (O’nyong’nyong). With the use of a differentiated primer, the cross-reaction was extinguished |
12 |
Investigate and identify viral etiology and advise health authorities on implementing control measures to contain an outbreak |
77 human plasma samples, collected at Hospital Severino de Souto Siqueira, in Recife, PE, between 25 and 31 May 2015 |
DENV CHIKV ZIKV |
31 (40.2%) patients with ZIKV infection; nine (11.7%) patients infected with DENV; one (1.3%) patient positive for CHIKV IgM, but negative in the PCR test. Coinfection by ZIKV/DENV was proven in 2.6% of patients. The study highlights the need for differentiation between viruses to improve health control measures |
13 |
Develop a specific, sensitive and robust assay in the RT-LAMP methodology for diagnosis and differentiation between DENV serotypes 1-4 |
190 serum samples |
DENV serotypes 1-4 |
RT-LAMP results were satisfactory; this methodology was more effective than traditional PCR and real-time PCR. Results (RT-LAMP, RT-PCR, qRT-PCR): sensitivity 98.9%, 84.2% and 90.5%, respectively; specificity 100%, 93% and 100%, respectively |
14 |
Develop two RT-RPA assays to detect DENV 1-4 |
Human samples from outbreaks in Senegal and Thailand |
DENV serotypes 1-4 |
Results (RT-RPA and qRT-PCR): sensitivity 98% RNA Thailand and 72% RNA Senegal; 98% RNA Thailand and 94.4% RNA Senegal. Specificity 100% and 100%, respectively |
15 |
Update the clinical and laboratory diagnosis of fever by VZIK |
blood, saliva and urine. Amniotic fluid, CSF, placenta and umbilical cord in cases of neonatal infection |
VZIK |
qPCR detects acute infection within seven days of symptom onset. Blood is less sensitive than urine (15 days) and saliva. Third day of manifestation the best time for testing. In neonates with clinical symptoms and negative PCR, perform serology |
16 |
Report a case of unilateral diaphragmatic paralysis in a neonate with a confirmed diagnosis of congenital zika by amniotic fluid examination using RT-PCR and by CSF serology |
Amniotic fluid and CSF |
DENV CHIKV ZIKV |
Positive RT-PCR in amniotic fluid at week 29 of gestation. Negative in maternal and newborn serum after delivery (acute infection during pregnancy). IgM in newborn serum confirms intrauterine infection (IgM does not cross placental barrier) |
17 |
Suggest that NS1 Elisa tests have low sensitivity and produce false negative results and cross reactions with other arboviruses |
150 human serum samples which tested DENV negative by Elisa |
DENV |
33 Elisa negative samples were positive for qRT-PCR, ie 22% false negatives were truly positive; 75% were from DENV-4. Correlation with other studies in which secondary DENV infection produces false negatives in NS1 kits |
18 |
Evaluate and optimize a diagnostic test using the qRT-PCR methodology for the Flavivirus genus |
410 serum samples with acute fever, DENV negative by RT-PCR |
DENV |
qRT-PCR is more sensitive and specific than traditional PCR, in addition to allowing virus quantification. There was no amplification of other Flavivirus, indicating that there is no cross-reaction |
19 |
Develop and evaluate serological methods for diagnosis and research of CHIKV in Nicaragua |
260 acute and convalescent serum samples |
CHIKV |
qRT-PCR was used as the gold standard. Seven PCR positive samples were serologically negative; four PCR negative and serology positive samples may indicate serology cross- reactivity. Low sensitivity of serology in the acute phase, being high for PCR in the same phase |
20 |
Report the detection of DENV and ZIKV by qRT-PCR in a pre-symptomatic blood donor, in Ribeirão Preto, São Paulo |
A blood sample (plasma) from the donor |
DENV ZIKV |
Detection of ZIKV infection concurrently with DENV infection. 13 million copies/ml of ZIKV and 5 million copies/ml of DENV-4 were found in the same blood donor plasma |