Using amniotic membrane for anal sphincter repair in animal model

Uso de membrana amniótica para reparo de esfíncter anal em modelo animal

Ahmad Izadpanah Mohammad Rezazadehkermani Mohammadali Ghaderi Salar Rahimikazerooni Alireza Safarpour Kaynoosh Homayouni Maral Mokhtari Alimohammad Bananzadeh About the authors

ABSTRACT

PURPOSE:

Sphincter repair is the primary management for fecal incontinence especially in traumatic causes. Regardless of progression in the method and material of sphincter repair, the results are still disappointing. This study evaluates the efficacy of using amniotic membrane during sphincteroplasty regarding its effects in healing of various tissues.

METHODS:

Rabbits undergone sphincterotomy and after three weeks end to end sphincteroplasty was done. Animals divided to three groups: classic sphincteroplasty, sphincteroplasty with fresh amniotic membrane and sphincteroplasty with decellularized amniotic membrane. Three weeks after sphincteroplasty animals were sacrificed and sphincter complex was sent for histopathologic evaluation. Sphincter muscle diameter and composition of sphincter was evaluated. Before sphincterotomy, before and after sphincteroplasty electromyography of sphincter at the site of repair were recorded.

RESULTS:

No statistical significant difference was seen between groups even in histopathology or electromyography.

CONCLUSION:

Although amniotic showed promising effects in the healing of different tissue in animal and human studies it was not effective in healing of injured sphincter.

Keywords:
Anal sphincter; Sphincteroplasty; Amniotic membrane; Electromyography; Fecal incontinence

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