Comparative study of 1,2-dimethylhydrazine and azoxymethane on the induction of colorectal cancer in rats

Estudo comparativo das substâncias 1,2-dimetil-hidrazina e azoximetano na indução de câncer colorretal em ratos

Mario Jorge Jucá Bruno Carneiro Bandeira Davi Silva Carvalho Antenor Teixeira Leal About the authors

The induced colorectal carcinogenesis in rodents has a long history and currently uses the substances 1,2-dimethylhydrazine and azoxymethane.

Objective:

The aim of this study was to compare the inductive effect of the substances azoxymethane and 1,2-dimethylhydrazine in colorectal carcinogenesis.

Method:

30 randomly chosen male Wistar rats were divided into four groups. G1 group was treated with 1,2-dimethylhydrazine and C1 was its control group; G2 group was treated azoxymethane and C2 was its control group. The animals were weekly weighed until euthanasia, when their intestines were removed, processed and analyzed by an experienced pathologist.

Results:

Among the control groups (C1 and C2) no histologic changes were observed; moderate dysplasia was detected in G2 group; hyperplasia, mild dysplasia, severe dysplasia and carcinoma were observed in G1 group. When this study compared the cost of the substances, 1,2-dimethylhydrazine was more than 50 times less expensive than azoxymethane.

Conclusion:

Azoxymethane is able to promote histological changes consistent with colorectal carcinogenesis. 1,2-Dimethylhydrazine produced neoplasia and dysplasia, and, compared to the azoxymethane, was more efficient in the induction of colorectal cancer.

Colorectal cancer; Experimental model; Carcinogenesis; Azoxymethane; 1,2-Dimethylhydrazine


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