X-ray imaging and digital processing application in non-destructive assessing of melon seed quality

Medeiros, André Dantas de; Martins, Maycon Silva; Silva, Laércio Junio da; Pereira, Márcio Dias; León, Manuel Jesús Zavala; Dias, Denise Cunha Fernandes dos Santos

doi:10.1590/2317-1545v42229761

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ARTICLE • J. Seed Sci. 42 • 2020 • https://doi.org/10.1590/2317-1545v42229761 copy

X-ray imaging and digital processing application in non-destructive assessing of melon seed quality

Aplicação do teste de raios-X e processamento digital na avaliação não-destrutiva da qualidade de sementes de melão

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract:

Non-destructive and high throughput methods have been developed for seed quality evaluation. The aim of this study was to relate parameters obtained from the free and automated analysis of digital radiographs of hybrid melons’ seeds to their seeds’ physiological potential. Seeds of three hybrid melon (Cucumis melo L.) cultivars from commercial lot samples were used. Radiographic images of the seeds were obtained, from which area, perimeter, circularity, relative density, integrated density and seed filling measurements were generated by means of a macro (PhenoXray) developed for ImageJ^® software. After the X-ray test, seed samples were submitted to the germination test, from which variables related to the physiological quality of the seeds were obtained. Variability between lots was observed for both physical and physiological characteristics. Results showed that the use of the PhenoXray macro allows large-scale phenotyping of seed radiographs in a simple, fast, consistent and completely free way. The methodology is efficient in obtaining morphometric and tissue integrity data of melon seeds and the generated parameters are closely related to physiological attributes of seed quality.

Index terms:
automated image analysis; Cucumis melo L.; seed radiography; relative density

ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br

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[1] *Corresponding author
E-mail: medeiros.seeds@gmail.com

Seed lot	Area	Per.	Circ.	Rel. dens.	Int. dens.	Filling
	mm²	mm	Circ.	gray.pixel^-1	gray.mm².pixel^-1	%
	Cultivar Bazuca F1
1	34.56 b	29.69 d	0.496 a	94.29 a	gray.mm².pixel^-1	%	3261 b	99 a
2	35.29 b	31.28 c	0.460 b	92.15 a	3254 b	98 a
3	30.18 c	31.92 c	0.401 c	88.63 b	2678 c	98 a
4	30.79 c	39.14 b	0.295 d	85.73 b	2637 c	96 b
5	39.50 a	32.50 c	0.474 b	97.04 a	3833 a	98 a
6	38.12 a	41.35 a	0.309 d	87.27 b	3328 b	95 c
7	38.05 a	42.38 a	0.302 d	85.72 b	3263 b	95 c
8	37.34 a	40.07 b	0.317 d	89.58 b	3344 b	95 c
9	38.35 a	41.05 a	0.314 d	88.61 b	3399 b	95 c
10	30.97 c	33.11 c	0.389 c	80.00 c	2479 c	94 d
Fc	46.60^*	52.08^*	65.51^*	10.01^*	28.60^*	78.53^*
CV (%)	3.28	4.23	5.78	3.83	5.55	0.49
	Cultivar Goldex F1
12	43.11 a	37.04 c	0.408 a	95.1	4102 a	98 a
12	41.67 b	38.18 c	0.395 a	88.76	3706 b	97 a
13	43.50 a	36.67 c	0.420 a	91.19	3970 a	97 a
14	43.37 a	35.84 c	0.441 a	93.69	4062 a	98 a
15	43.80 a	36.07 c	0.445 a	89.28	3914 a	98 a
16	42.50 b	49.80 b	0.254 b	88.31	3762 b	94 c
17	41.93 b	50.32 b	0.243 b	86.32	3622 b	93 c
18	42.46 b	49.88 b	0.266 b	87.01	3707 b	93 c
19	43.89 a	46.89 b	0.287 b	92.89	4081 a	96 b
20	42.51 b	53.60 a	0.234 b	84.54	3603 b	93 c
Fc	4.11^*	30.40^*	25.24^*	1.50^ns	2.27^*	84.26^*
CV (%)	1.99	6.76	11.71	6.95	7.5	0.52
	Cultivar Pampa F1
21	29.19 d	37.17	0.349 b	90.78 b	2665 b	95 a
22	34.26 b	37.38	0.333 b	91.43 b	3136 a	94 b
23	32.52 c	34.67	0.356 b	97.97 a	3195 a	95 a
24	34.25 b	34.4	0.376 a	102.15 a	3502 a	96 a
25	35.17 b	35.03	0.382 a	99.89 a	3531 a	96 a
26	37.31 a	35.5	0.399 a	88.69 b	3313 a	96 a
27	36.67 a	34.59	0.409 a	89.66 b	3291 a	96 a
28	37.27 a	35.13	0.403 a	89.01 b	3324 a	96 a
29	38.00 a	35.9	0.399 a	89.40 b	3402 a	96 a
30	32.10 c	40.6	0.288 b	78.80 c	2532 b	95 b
Fc	42.50^*	1.04^ns	3.69^*	4.54^*	9.54^*	5.39^*
CV (%)	2.77	11.58	12.06	7.7	7.62	0.82

Seed lot	Germ.	Rad. pro.	GSI	RPS	Sync	SL	Unif.	Vigor	Corr. vigor
	%					mm	Index
	Cultivar Bazuca F1
1	95 a	100 a	6.33 a	11.8 b	1.00 a	9.24 c	892 a	770 b	731 b
2	94 a	100 a	6.27 a	14.2 a	1.00 a	9.50 c	871 a	768 b	722 b
3	95 a	97 a	6.18 a	12.0 b	0.90 b	9.41 c	868 a	763 b	725 b
4	86 b	90 b	5.54 b	10.9 b	0.84 b	8.08 d	790 b	678 c	583 c
5	97 a	98 a	6.30 a	11.4 b	0.88 b	9.47 c	882 a	772 b	749 b
6	94 a	99 a	5.97 a	11.2 b	0.69 c	12.56 a	835 b	857 a	806 a
7	85 b	100 a	5.23 b	10.6 b	0.60 c	11.45 b	817 b	809 b	688 b
8	95 a	99 a	5.90 a	10.0 b	0.61 c	12.35 b	856 a	860 a	817 a
9	93 a	99 a	5.57 a	11.0 b	0.54 d	11.86 a	866 a	848 a	789 a
10	64 c	76 c	3.75 c	7.4 c	0.47 d	8.96 c	660 c	644 c	412 d
Fc	9.15^*	23.38^*	16.44^*	13.73^*	17.67^*	28.30^*	12.46^*	14.05^*	52.51^*
CV (%)	8.15	3.65	7.55	9.3	13.8	6.53	5.2	5.58	5.36
	Cultivar Goldex F1
11	98 a	100 a	6.45 a	19.3 a	0.91 a	13.36 a	864 a	899 a	881 a
12	98 a	99 a	6.14 a	18.5 a	0.67 b	10.83 b	864 a	811 b	795 b
13	96 a	100 a	6.19 a	17.4 b	0.82 a	12.41 a	857 a	863 a	828 b
14	99 a	100 a	6.26 a	18.2 a	0.69 b	12.66 a	874 a	880 a	871 a
15	93 a	98 a	5.77 b	17.4 b	0.60 b	12.00 b	816 b	828 b	770 b
16	89 b	99 a	5.62 b	14.1 c	0.65 b	11.36 b	795 b	795 b	708 c
17	89 b	98 a	5.75 b	12.4 d	0.79 a	11.79 b	813 b	819 b	729 c
18	85 b	100 a	5.47 b	14.6 c	0.75 b	11.23 b	748 c	767 b	652 d
19	91 b	97 a	6.02 a	12.0 d	0.94 a	11.93 b	806 b	820 b	747 c
20	84 b	93 b	5.30 b	9.1 e	0.65 b	10.84 b	747 c	753 b	633 d
Fc	4.18^*	3.89^*	4.48^*	57.22^*	4.88^*	3.45^*	4.66^*	3.87^*	15.54^*
CV (%)	7.11	2.26	7.31	6.51	15.49	8.28	5.86	6.44	6.32
	Cultivar Pampa F1
21	96 a	100 a	6.24 a	19.4 a	0.88	11.77 c	849 b	836 b	803 b
22	94 a	97 b	5.98 b	18.4 b	0.78	11.50 d	809 b	807 c	759 c
23	97 a	100 a	6.29 a	19.7 a	0.86	12.96 b	816 b	861 b	836 b
24	100 a	100 a	6.61 a	20.0 a	0.94	14.54 a	917 a	967 a	967 a
25	98 a	100 a	6.44 a	18.0 b	0.92	13.93 a	898 a	936 a	918 a
26	91 b	99 a	5.88 b	19.0 a	0.78	12.11 c	778 c	813 c	740 c
27	87 b	99 a	5.55 b	19.2 a	0.71	11.41 d	758 c	778 c	677 d
28	97 a	100 a	6.28 a	18.8 a	0.79	12.43 c	825 b	848 b	822 b
29	97 a	100 a	6.35 a	17.6 b	0.87	12.25 c	840 b	849 b	823 b
30	92.5 b	100 a	6.00 b	10.7 c	0.81	10.70 d	815 b	782 c	719 c
Fc	4.05^*	2.69^*	4.93^*	62.49^*	1.83^ns	11.93^*	6.40^*	9.32^*	21.18^*
CV (%)	4.51	1.33	5.05	4.22	14.07	6.14	5.2	5.35	5.34