Use of radiographic images for rapid and non-destructive assessment of crambe seed quality

Ribeiro, Alessandra da Silva; Neri, Tássia Fernanda Santos; Medeiros, André Dantas de; Milagres, Carla do Carmo; Silva, Laércio Junio da

doi:10.1590/2317-1545v43239136

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ARTICLE • J. Seed Sci. 43 • 2021 • https://doi.org/10.1590/2317-1545v43239136 copy

Use of radiographic images for rapid and non-destructive assessment of crambe seed quality

Uso de imagens radiográficas para avaliação rápida e não destrutiva da qualidade de sementes de crambe

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract:

Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ^® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.

Index terms:
Crambe abyssinica; image analysis; relative density; seed radiography

ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br

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[1] *Corresponding author
E-mail: carlacmilagres@gmail.com

Structure		Characteristic	Description
Seed	Embryo
AreaSeed	AreaEmb	Area (mm²)	Area of the seed or embryo.
PerSeed	PerEmb	Perimeter (mm)	Perimeter of the seed or embryo - the outline of each seed in the image.
CircSeed	CircEmb	Circularity	Seed or embryo circularity - the shape of the seed being 1, completely roundly, and 0, not roundly.
RelDensSeed	RelDensEmb	Relative Density (gray.pixel^-1)	Mean gray in the seed or embryo - the mean value of each pixel comprising the seed in a monochromatic image, ranging from 0 (black) to 255 (white).
IntDenSeed	IntDenEmb	Integrated density (gray.mm².pixel^-1)	Product of the area of the seed or embryo with the relative density.
SolSeed	SolEmb	Solidity	Relation between area and convex area. Regular shape = 1, irregular shape < 1.
Filling		Seed Filling	Percentage of area of the embryo in relation to the area of the seed (%).

Seed Lot	Area	Perimeter	Circularity	Relative density	Integrated density	Solidity	Seed filling
Unit	mm²	mm		gray.pixel^-1	gray.mm².pixel^-1
Seed
1	7.37 b^a	11.38 a	0.75 b	89.32 c	664 b	0.976 c	50.07 a
2	6.70 d	9.90 c	0.86 a	94.16 b	631 b	0.983 b	51.42 a
3	6.61 d	9.82 c	0.86 a	98.03 a	648 b	0.983 b	52.47 a
4	7.23 b	10.20 b	0.87 a	87.22 c	632 b	0.984 a	42.24 c
5	6.96 c	10.18 b	0.85 a	99.95 a	697 a	0.982 b	51.02 a
6	7.06 c	10.25 b	0.85 a	100.72 a	710 a	0.983 b	52.12 a
7	7.24 b	10.42 b	0.84 a	101.88 a	737 a	0.982 b	53.06 a
8	7.27 b	10.30 b	0.86 a	101.52 a	738 a	0.983 b	52.33 a
9	7.82 a	10.63 b	0.87 a	91.84 b	718 a	0.984 a	45.28 b
10	7.16 b	10.27 b	0.85 a	97.00 a	695 a	0.983 b	51.35 a
Fc	8.65*	12.79*	26.69*	13.51*	10.76*	25.64*	32.49*
CV	3.28	2.35	1.65	2.98	3.63	0.09	2.48
Embryo
1	3.69 a	8.21 a	0.69 a	146.54 b	549 b	0.958 a	-
2	3.44 b	7.82 b	0.71 a	147.65 b	509 c	0.960 a	-
3	3.47 b	7.69 b	0.74 a	150.02 b	522 c	0.965 a	-
4	3.05 c	8.21 a	0.59 b	144.71 b	451 d	0.927 b	-
5	3.55 b	8.25 a	0.67 a	154.30 a	549 b	0.958 a	-
6	3.68 a	8.24 a	0.69 a	153.34 a	564 b	0.963 a	-
7	3.84 a	8.27 a	0.72 a	154.04 a	592 a	0.966 a	-
8	3.80 a	8.30 a	0.70 a	154.62 a	589 a	0.965 a	-
9	3.53 b	8.53 a	0.63 b	147.03 b	526 c	0.948 a	-
10	3.67 a	8.27 a	0.69 a	150.16 b	553 b	0.961 a	-
Fc	17.03*	5.86*	9.89*	6.48*	24.62*	10.40*	-
CV	3.07	2.45	4.07	1.88	3.07	0.76	-

Seed Lot	Ger	RadProt	MGT	MRPT	T₅₀	GSI	RPSI	Sync.
Unit	---% ---		---- days ----			--- Index ---
1	67 ba	83 b	4.53	2.17 b	3.94	7.71 b	26.64 a	0.68 a
2	78 b	95 a	4.60	2.62 b	3.97	8.67 b	19.55 a	0.51 b
3	92 a	99 a	4.23	2.41 b	3.60	10.96 a	21.67 a	0.70 a
4	26 d	33 d	4.86	3.61 a	4.17	2.77 d	5.09 b	0.33 b
5	90 a	99 a	4.24	2.57 b	3.64	10.75 a	20.66 a	0.70 a
6	93 a	99 a	4.27	2.55 b	3.63	11.05 a	20.52 a	0.66 a
7	86 a	97 a	4.35	2.55 b	3.70	10.08 a	20.17 a	0.60 a
8	90 a	100 a	4.44	2.82 b	3.80	10.29 a	18.86 a	0.50 b
9	48 c	56 c	4.67	3.46 a	3.99	5.28 c	9.19 b	0.41 b
10	88 a	93 a	4.37	2.80 b	3.77	10.15 a	17.88 a	0.54 b
Fc	27.93*	36.20*	1.91	4.70*	1.56	21.47*	7.97*	3.30*
CV	11.22	8.92	6.74	15.13	7.94	13.64	24.64	25

Seed lots	Hypocotyl length	Root length	Seedling length	Uniformity	Growth	Vigor	Corrected vigor
unit	----cm----			-------- index --------
1	1.04 ba	0.93 b	1.97 c	434 b	94 b	196 b	135 b
2	1.72 a	2.43 a	4.15 a	576 a	236 a	338 a	267 a
3	1.72 a	2.82 a	4.54 a	657 a	271 a	387 a	355 a
4	0.90 b	0.49 b	1.39 c	267 c	53 b	117 c	31 c
5	1.95 a	2.75 a	4.70 a	615 a	267 a	372 a	336 a
6	2.03 a	2.82 a	4.85 a	620 a	274 a	378 a	352 a
7	1.76 a	2.76 a	4.51 a	579 a	266 a	360 a	313 a
8	1.90 a	2.69 a	4.59 a	632 a	261 a	373 a	336 a
9	1.74 a	1.28 b	3.01 b	444 b	132 b	226 b	111 b
10	1.84 a	2.55 a	4.39 a	665 a	248 a	373 a	326 a
Fc	13.24*	7.56*	10.14*	16.23*	7.90*	15.09*	16.28*
CV	12.54	30.17	20.43	11.56	28.32	15.82	22.91