Adaptation of the tetrazolium test for assessing the seed quality of <i>Crateva tapia</i> L.

Rodrigues, Marília Hortência Batista Silva; Alves, Edna Ursulino; Barbosa, Luana da Silva; Silva, Joyce Naiara da; Bezerra, Ana Carolina; Pádua, Guilherme Vinícius Gonçalves de

doi:10.1590/2317-1545v47296471

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ARTICLE • J. Seed Sci. 47 • 2025 • https://doi.org/10.1590/2317-1545v47296471 linkcopy

Adaptation of the tetrazolium test for assessing the seed quality of Crateva tapia L.

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ABSTRACT:

In the seed sector, demand for quick decisions is growing, especially for slow-germinating species such as Crateva tapia L., so the tetrazolium test stands out as a method capable of evaluating seed viability and vigor. The objective was to adapt the tetrazolium test to evaluate the viability and vigor of C. tapia seeds. A completely randomized design was used, with a 2 x 4 x 5 factorial scheme (two lots of seeds, four concentrations of 2, 3, 5 triphenyl tetrazolium chloride salt (0.025; 0.050; 0.075 and 0.1%) and five times of immersion (90; 180; 270; 360 and 450 minutes). The initial characterization of the lots was carried out (physical and physiological quality) and different concentrations and immersion periods were applied, classifying them into class I (viable and vigorous embryos), II (viable seeds) and III (non-viable). Lot I showed greater quality and vigor, confirmed by the 0.075% tetrazolium test for 270 minutes. The concentration of 0.1% made it difficult to interpret the results. Color varies between light pink and white, with the 0.075% tetrazolium salt concentration for 270 minutes of immersion being recommended to evaluate the viability of C. tapia seeds.

Index terms:
forest seeds; rapid test; trapiá; viability; vigor

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ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br

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[TFN1] Note. Means followed by the same lowercase letter in the column do not differ statistically from each other by Tukey test at 5% probability level; CV: coefficient of variation.

Lots	MC (%)	SDM (mg)	FGC (%)	GP (%)	GSI (index)	MGT (days^-1)	MGS (days^-1)
I	12.38 a	3.58 a	67 a	95 a	1.58 a	15.01 b	0.066 a
II	11.64 b	2.96 a	26 b	90 a	0.92 b	17.43 a	0.057 b
CV (%)	2.30	1.98	4.51	4.23	4.81	4.19	3.59
Lots	SL cm	RL cm	TL cm	SHDM g	RDM g	TDM g
I	7.93 a	9.71 a	17.14 a	0.0301 a	0.0159 a	0.046 a
II	5.01 b	9.21 a	14.72 b	0.017 b	0.0121 a	0.0293 b
CV (%)	6.92	5.13	5.14	10.41	16.78	11.38

		Mean Squares
		Class I	Class II	Class III
			(%)
Source of variation	DF
Lot (L)	1	61308.900**	313.600**	66585.600**
Concentration (C)	3	3445.700**	2705.466**	10158.666**
Time (T)	4	23019.400**	6895.000**	30834.850**
L x C	3	10927.033**	251.733**	8661.600**
L x T	4	5471.400**	490.600**	3636.600**
C x T	12	3201.200**	2108.466**	1431.916**
L x C x T	12	1668.533**	510.733**	1515.683**
Residual	120	9.833	5.400	59.200
Total	159
CV (%)		6.13	28.17	19.19
Mean		51.17	8.25	40.10

	Lot I
	Time of immersion in tetrazolium salt (minutes)
Tetrazolium concentrations (%)	90	180	270	360	450
0.025	6.0 cCα	93.0 bBα	91.0 bBα	98.0 aAα	95.0 aAα
0.050	10.0 bCα	93.0 bBα	100.0 aAα	89.0 bBα	92.0 aBα
0.075	4.0 cBα	97.0 aAα	100.0 aAα	98.0 aAα	8.0 bBβ
0.1	24.0 aDα	97.0 aAα	96.0 aAα	92.0 bBα	32.0 bCα
	Lot II
	Time of immersion in tetrazolium salt (minutes)
Tetrazolium concentrations (%)	90	180	270	360	450
0.025	0.0 aBβ	0.0 dBβ	0.0 cBβ	10.0 cAβ	9.0 dAβ
0.050	0.0 aCβ	5.0 cBβ	10.0 bBβ	8.0 cBβ	77.0 aAβ
0.075	3.0 aDα	79.0 aBβ	87.0 aAβ	80.0 aBβ	71.0 bCα
0.1	0.0 aDβ	15.0 bCβ	89.0 aAβ	75.0 bBβ	14.0 cCβ

	Lot I
	Time of immersion in tetrazolium salt (minutes)
Tetrazolium concentrations (%)	90	180	270	360	450
0.025	0.00 aBα	0.00 aBα	5.00 aAα	0.00 bBα	1.00 dBα
0.050	0.00 aBα	1.00 aBα	0.00 bBα	9.00 aAα	8.00 bAα
0.075	0.00 aBα	0.00 aBα	0.00 bBβ	1.00 bBβ	92.00 aAα
0.1	0.00 aCα	0.00 aCα	2.00 bCα	6.00 aBβ	68.00 cAβ
	Lot II
	Time of immersion in tetrazolium salt (minutes)
Tetrazolium concentrations (%)	90	180	270	360	450
0.025	0.00 aAα	0.00 aAα	0.00 bAβ	0.00 bAα	0.00 cAα
0.050	0.00 aAα	0.00 aAα	0.00 bAα	0.00 bAβ	0.00 cAβ
0.075	0.00 aDα	0.00 aDα	6.00 aCα	13.00 aBα	28.00 bAβ
0.1	0.00 aCα	0.00 aCα	0.00 bCα	14.00 aBα	76.00 aAα

	Lot I
	Time of immersion in tetrazolium salt (minutes)
Tetrazolium concentrations (%)	90	180	270	360	450
0.025	94.00 aAα	7.00 aBβ	4.00 aBβ	2.00 aBβ	4.00 aBβ
0.050	90.00 aAα	6.00 aBβ	0.00aBβ	2.00 aBβ	0.00 aBβ
0.075	96.00aAα	3.00 aBβ	0.00 aBα	1.00 aBα	0.00 aBα
0.1	76.00 bAβ	3.00 aBβ	3.00 aBα	2.00 aBα	1.00 aBα
	Lot II
	Time of immersion in tetrazolium salt (minutes)
Tetrazolium concentrations (%)	90	180	270	360	450
0.025	100.00 aAα	77.00 bBα	100.00 aAα	91.00 aAα	91.00 aAα
0.050	100.00 aAα	95.00 aAα	90.00 aAα	93.00 aAα	23.00 bBα
0.075	97.00 aAα	21.00 cBα	7.00 bCα	7.00 bCα	1.00 cCα
0.1	100.00 aAα	85.00 bBα	11.00 bCα	11.00 bCα	10.00 cCα