Enhancing cadmium and salinity stress tolerance in upland rice through priming: unraveling the impact of signaling molecules

ABSTRACT:

Priming associated with signaling molecules is an effective technique to promote uniform and rapid germination in the field, in addition to increasing stress tolerance. This study aimed to evaluate the effect of priming solutions on tolerance to cadmium and salinity stress in two upland rice cultivars, Douradão and Soberana. Five priming agents were tested: indole acetic acid (IAA), melatonin, sodium nitroprusside (SNP), hydrogen peroxide (H₂O₂) and chitosan. After treatment, the seeds were subjected to three germination conditions: control (no stress), saline stress and cadmium stress. Evaluations included seed moisture content, germination percentage, seedling growth and activity of antioxidant enzymes (superoxide dismutase - SOD, catalase - CAT and ascorbate peroxidase - APX). The results showed that the effects of priming varied according to the type of stress and the agent used. Under saline stress, H₂O₂ favored germination and root growth, while under cadmium stress, IAA, melatonin and H₂O₂ were more effective. Chitosan induced high SOD activity in the cadmium stress, and SNP stood out for salinity. H₂O₂ increased APX activity in the cultivar Soberana, while SNP was more effective for Douradão in both stress conditions. Catalase was activated by H₂O₂ and melatonin. The study concludes that SNP, H₂O₂ and IAA can improve stress tolerance during rice seed germination by activating antioxidant systems that favor growth under adverse conditions.

Index terms:
antioxidant system; germination; Oryza sativa L.; oxidative stress; seedling growth